Ionic liquids strongly affect the interaction of bacteria with magnesium oxide and silica nanoparticles

Borkowski, Andrzej; Syczewski, Marcin; Czarnecka-Skwarek, Anna

doi:10.1039/c9ra05110d

Cited by 7 publications

(6 citation statements)

References 53 publications

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“…However, silica probably served as a scaffold for this process that could be observed in the microscopic analysis ( Figure 3 ). A similar effect was observed by Borkowski et al [ 37 ], who detected the increased interaction of cells with silica nanoparticles in the presence of positively charged ionic liquids. The role of silica in our studies can also be supported by the fact that we did not observe such a strong agglomeration effect in the additional samples that contained only TiO 2 .…”

Section: Discussionsupporting

confidence: 86%

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

Augustyniak

Cendrowski

Grygorcewicz

et al. 2020

IJMS

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Pseudomonas aeruginosa is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of P. aeruginosa PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the mexA coding efflux pump was upregulated, which could be associated with an interaction with TiO2. The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of P. aeruginosa PAO1.

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Section: Discussionsupporting

confidence: 86%

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

Augustyniak

Cendrowski

Grygorcewicz

et al. 2020

IJMS

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“…The examples from the literature concern the lower activity of DEH in P. aeruginosa and S. aureus treated with Ag-NPs [35], a stimulation of the DEH activity in E. coli and B. cereus using SiC nanofibers as well as the inhibition of this enzyme in B. cereus using SiC/Ag/CE/T [21]. In another study, Borkowski et al [36] reported that MgO-NPs strongly stimulated the DEH activity in E. coli and B. cereus, while SiO 2 -NPs only affected the E. coli enzymes. The provided examples clearly indicate the diverse impact of tested NMs on various bacterial strains.…”

Section: Discussionmentioning

confidence: 99%

Impact of an Engineered Copper-Titanium Dioxide Nanocomposite and Parent Substrates on the Bacteria Viability, Antioxidant Enzymes and Fatty Acid Profiling

Metryka

Wasilkowski

Nowak

et al. 2020

IJMS

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Due to the systematic increase in the production of nanomaterials (NMs) and their applications in many areas of life, issues associated with their toxicity are inevitable. In particular, the performance of heterogeneous NMs, such as nanocomposites (NCs), is unpredictable as they may inherit the properties of their individual components. Therefore, the purpose of this work was to assess the biological activity of newly synthesized Cu/TiO2-NC and the parent nanoparticle substrates Cu-NPs and TiO2-NPs on the bacterial viability, antioxidant potential and fatty acid composition of the reference Escherichia coli and Bacillus subtilis strains. Based on the toxicological parameters, it was found that B. subtilis was more sensitive to NMs than E. coli. Furthermore, Cu/TiO2-NC and Cu-NPs had an opposite effect on both strains, while TiO2-NPs had a comparable mode of action. Simultaneously, the tested strains exhibited varied responses of the antioxidant enzymes after exposure to the NMs, with Cu-NPs having the strongest impact on their activity. The most considerable alternations in the fatty acid profiles were found after the bacteria were exposed to Cu/TiO2-NC and Cu-NPs. Microscopic images indicated distinct interactions of the NMs with the bacterial outer layers, especially in regard to B. subtilis. Cu/TiO2-NC generally proved to have less distinctive antimicrobial properties on B. subtilis than E. coli compared to its parent components. Presumably, the biocidal effects of the tested NMs can be attributed to the induction of oxidative stress, the release of metal ions and specific electrochemical interactions with the bacterial cells.

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“…The metabolic activity of the bacteria was determined based on the dehydrogenases activity using the method previously successfully tested and reported by Borkowski et al [ 25 ]. In the experiment, the 12-h cultures of E. coli and B. cereus bacteria with the growth densities of 0.594 and 1.24 equal to 227 µg of protein mL −1 and 230 µg of protein mL −1 , respectively, were used.…”

Section: Methodsmentioning

confidence: 99%

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

et al. 2020

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Carbon-based (nano)materials doped with transition metals, nitrogen and other heteroatoms are considered active heterogeneous catalysts in a wide range of chemical processes. Recently they have been scrutinized as artificial enzymes since they can catalyze proton-coupled electron transfer reactions vital for living organisms. Herein, interactions between Gram-positive and Gram-negative bacteria and either metal-free N and/or S doped or metal containing Fe−N−S co-doped porous carbons are studied. The Fe- and N-co-doped porous carbons (Fe−N−C) exhibit enhanced affinity toward bacteria as they show the highest adsorption capacity. Fe−N−C materials also show the strongest influence on the bacteria viability with visible toxic effect. Both types of bacteria studied reacted to the presence of Fe-doped carbons in a similar manner, showing a decrease in dehydrogenases activity in comparison to controls. The N-coordinated iron-doped carbons (Fe−N−C) may exhibit oxidase/peroxidase-like activity and activate O2 dissolved in the solution and/or oxygen-containing species released by the bacteria (e.g., H2O2) to yield highly bactericidal reactive oxygen species. As Fe/N/ and/or S-doped carbon materials efficiently adsorb bacteria exhibiting simultaneously antibacterial properties, they can be applied, inter alia, as microbiological filters with enhanced biofouling resistance.

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Ionic liquids strongly affect the interaction of bacteria with magnesium oxide and silica nanoparticles

Abstract: The antibacterial properties of nanoparticles can be strongly affected by interactions with ionic liquids.

Cited by 7 publications

References 53 publications

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes

Impact of an Engineered Copper-Titanium Dioxide Nanocomposite and Parent Substrates on the Bacteria Viability, Antioxidant Enzymes and Fatty Acid Profiling

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

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