Ionic Liquid Modification Optimizes the Interface between Lipase and Magnetic GO for Enhancing Biocatalysis

Abstract

In this study, imidazolium-based ionic liquids (ILs) were used to modify carboxymethyl cellulose-coated Fe 3 O 4 nanoparticles with graphene oxide deposition (ILs-MCMC/GO). The prepared composites were characterized and used to immobilize lipase. The prepared biocatalyst (PPL-ILs-MCMC/GO) exhibited higher activity, which was 3.55-fold that of free lipase and 1.41-fold that of immobilized lipase without IL (PPL-MCMC/GO). After reusing 10 times, the residual activity of PPL-ILs-MCMC/GO was 91.3%, which was higher than those of PPL-MCMC/GO (83.6%) and PPL-MCMC (73.6%). In addition, using kinetic analysis, we showed that the affinity between PPL-ILs-MCMC/GO and the substrate was improved. The secondary structures of free and immobilized lipase were analyzed to explain the mechanism by which the performance of PPL-ILs-MCMC/GO was improved. The introduction of imidazole-based IL was useful in improving the microenvironment of immobilized lipase. Furthermore, magnetic supports loaded with lipase could be recovered easily.

Covalent immobilization of lipase on an ionic liquid-functionalized magnetic Cu-based metal–organic framework with boosted catalytic performance in flavor ester synthesis

“…The improvement may be due to the interaction between the carrier and urea molecules, which prevented urea from disrupting the structure of lipase. 43 On the other hand, the multiple interactions between the enzyme and carrier strengthened the rigidity and integrity of lipase, which was further analyzed in the secondary structure investigation.…”

Ionozymes for Efficient Synthesis of Cadaverine: Offering a Sustainable Way for Bio-nylon 5X Production

“…Both the choline-based ILs and tetramethylguanidine-based ILs have been reported to have good biocompatibility for enzymatic catalysis. , In addition, some imidazolium-based ILs with short side chains owned preferable bioactivity in favor of the stability of protein . In this study, 37 kinds of ILs (including choline-based ILs, imidazolium-based ILs, and other types of ILs) have been applied to enhance the catalytic activity of ΔLdcEt3.…”

“…41,42 In addition, some imidazolium-based ILs with short side chains owned preferable bioactivity in favor of the stability of protein. 43 In In addition, the binary ILs−solvent systems demonstrate a great synergetic effect favoring the improvement of catalytic efficiency, 45 and the IL−IL synergetic systems also have been reported to regulate the substrate affinity 20 and improve the activity and stability of enzymes. 31,46 Structural Changes and Aggregation of Ionozymes ΔLdcEt3-ILs.…”

Preparation and Characterization of Magnetic Metal–Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase

“…The K m values of PPL-Fe 3 O 4 @UiO-66-NH 2 and PPL-IL-Fe 3 O 4 @UiO-66-NH 2 were 75.48 and 71.74, respectively. The K m value of PPL-IL-Fe 3 O 4 @UiO-66-NH 2 was slightly lower, indicating that PPL-IL-Fe 3 O 4 @UiO-66-NH 2 had a stronger affinity to the substrate [ 45 ]. The V max of PPL-IL-Fe 3 O 4 @UiO-66-NH 2 was higher than that of PPL-Fe 3 O 4 @UiO-66-NH 2 , showing that the substrate had a strong affinity with PPL-IL-Fe 3 O 4 @UiO-66-NH 2 and high catalytic efficiency.…”