Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli

Yee, Matthew; G, Emmel; Yang, Eric J.; Lee, Eumene; Paek, Justin H.; Wu, Benjamin M.; Kamei, Daniel T.

doi:10.3389/fchem.2018.00486

Cited by 13 publications

(5 citation statements)

References 26 publications

(34 reference statements)

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“…These devices help to detect antigens, antibodies, and nucleic acids in saliva, blood, and phlegm. Point-of-care biosensors test these samples based on fluorescent, colorimetric, or electrochemical detection techniques [ 8 , 9 ]. Such devices offer many advantages compared to the other techniques used for detection, such as higher sensitivity, lower cost, high specificity, less time-intensive process, and greater user-friendliness.…”

Section: Developments In Sars-cov-2 Diagnostic Strategiesmentioning

confidence: 99%

SARS-CoV-2-on-Chip for Long COVID Management

et al. 2022

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a “wicked evil” in this century due to its extended progression and huge human mortalities. Although the diagnosis of SARS-CoV-2 viral infection is made simple and practical by employing reverse transcription polymerase chain reaction (RT-PCR) investigation, the process is costly, complex, time-consuming, and requires experts for testing and the constraints of a laboratory. Therefore, these challenges have raised the paradigm of on-site portable biosensors on a single chip, which reduces human resources and enables remote access to minimize the overwhelming burden on the existing global healthcare sector. This article reviews the recent advancements in biosensors for long coronavirus disease (COVID) management using a multitude of devices, such as point-of-care biosensors and lab-on-chip biosensors. Furthermore, it details the shift in the paradigm of SARS-CoV-2-on-chip biosensors from the laboratory to on-site detection with intelligent and economical operation, representing near-future diagnostic technologies for public health emergency management.

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Section: Developments In Sars-cov-2 Diagnostic Strategiesmentioning

confidence: 99%

SARS-CoV-2-on-Chip for Long COVID Management

et al. 2022

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“…In a study focused on IL-based ABSs for extracting transferrin, Yee et al [59] used an ABS comprising 1-butyl-3-methylimidazolium tetrafluoroborate ([C4C1im][BF4]) and sodium phosphate to extract transferrin for point-of-care diagnostic purposes. Although the authors achieved a 20-fold enrichment factor, no extraction efficiencies or recovery yields were reported; therefore, a direct comparison was not possible.…”

Section: Extraction Of Transferrinmentioning

confidence: 99%

Aqueous Biphasic Systems Comprising Natural Organic Acid-Derived Ionic Liquids

et al. 2022

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Despite the progress achieved by aqueous biphasic systems (ABSs) comprising ionic liquids (ILs) in extracting valuable proteins, the quest for bio-based and protein-friendly ILs continues. To address this need, this work uses natural organic acids as precursors in the synthesis of four ILs, namely tetrabutylammonium formate ([N4444][HCOO]), tetrabutylammonium acetate ([N4444][CH3COO]), tetrabutylphosphonium formate ([P4444][HCOO]), and tetrabutylphosphonium acetate ([P4444][CH3COO]). It is shown that ABSs can be prepared using all four organic acid-derived ILs paired with the salts potassium phosphate dibasic (K2HPO4) and tripotassium citrate (C6H5K3O7). According to the ABSs phase diagrams, [P4444]-based ILs outperform their ammonium congeners in their ability to undergo liquid–liquid demixing in the presence of salts due to their lower hydrogen-bond acidity. However, deviations to the Hofmeister series were detected in the salts’ effect, which may be related to the high charge density of the studied IL anions. As a proof of concept for their extraction potential, these ABSs were evaluated in extracting human transferrin, allowing extraction efficiencies of 100% and recovery yields ranging between 86 and 100%. To further disclose the molecular-level mechanisms behind the extraction of human transferrin, molecular docking studies were performed. Overall, the salting-out exerted by the salt is the main mechanism responsible for the complete extraction of human transferrin toward the IL-rich phase, whereas the recovery yield and protein-friendly nature of these systems depend on specific “IL-transferrin” interactions.

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“…In this work, the authors have synthesised the PIPs on the surface of gold nanorods, an essential aspect that enhances the Raman scattering effect, and ensured the polymeric film was sufficiently thin (5.3 ± 0.4 nm) to support favorable binding kinetics. The period between protein incubation and conducting the Raman measurements was 20 min, with 4.61 mg/L being the lowest transferrin concentration assessed using this sensing approach, which is considerably less sensitive than some comparable techniques [164,165,166].…”

Section: Detection Techniquesmentioning

confidence: 99%

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Regan

Walsh

O’Kennedy

et al. 2019

Sensors

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Molecular imprinting is a rapidly growing area of interest involving the synthesis of artificial recognition elements that enable the separation of analyte from a sample matrix and its determination. Traditionally, this approach can be successfully applied to small analyte (<1.5 kDa) separation/ extraction, but, more recently it is finding utility in biomimetic sensors. These sensors consist of a recognition element and a transducer similar to their biosensor counterparts, however, the fundamental distinction is that biomimetic sensors employ an artificial recognition element. Molecularly imprinted polymers (MIPs) employed as the recognition elements in biomimetic sensors contain binding sites complementary in shape and functionality to their target analyte. Despite the growing interest in molecularly imprinting techniques, the commercial adoption of this technology is yet to be widely realised for blood sample analysis. This review aims to assess the applicability of this technology for the point-of-care testing (POCT) of cardiovascular disease-related biomarkers. More specifically, molecular imprinting is critically evaluated with respect to the detection of cardiac biomarkers indicative of acute coronary syndrome (ACS), such as the cardiac troponins (cTns). The challenges associated with the synthesis of MIPs for protein detection are outlined, in addition to enhancement techniques that ultimately improve the analytical performance of biomimetic sensors. The mechanism of detection employed to convert the analyte concentration into a measurable signal in biomimetic sensors will be discussed. Furthermore, the analytical performance of these sensors will be compared with biosensors and their potential implementation within clinical settings will be considered. In addition, the most suitable application of these sensors for cardiovascular assessment will be presented.

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Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli

Cited by 13 publications

References 26 publications

SARS-CoV-2-on-Chip for Long COVID Management

SARS-CoV-2-on-Chip for Long COVID Management

Aqueous Biphasic Systems Comprising Natural Organic Acid-Derived Ionic Liquids

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

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