Ionic gelated β-cyclodextrin-biotin-carboxymethyl chitosan nanoparticles prepared as carrier for oral delivery of protein drugs

Abstract

In this paper, the β-cyclodextrin (β-CD) and biotin (Bi) were successfully grafted onto carboxymethyl chitosan (CMCS). And then the β-CD-Bi-CMCS nanoparticles (NPs) were prepared as oral nano-delivery carrier of protein drugs by ionic gelation method. The morphological feature of fabricated drug carrier was determined by dynamic light scattering and transmission electron microscopy. The result showed that the prepared NPs presented spherical structure with an average diameter of 138 nm. Bovine serum albumin (BSA) was selected as model protein drug that was entrapped in prepared drug carrier with satisfactory entrapment efficiency (79.18%) and loading content (3.96%). The drug release profiles of BSA/β-CD-Bi-CMCS NPs were studied at different pH environment for simulated gastric fluid (SGF), simulated intestinal fluid (SIF) and simulated colonic fluid (SCF). It was found that the BSA/β-CD-Bi-CMCS NPs displayed a pH dependent drug release profiles. After 72 h, the cumulative release amount of BSA in SGF, SIF, and SCF was about 20.57, 74.46, and 91%, respectively. Furthermore, the enzymatic degradation and cytotoxicity studies showed the synthesized β-CD-Bi-CMCS NPs had high chemical stability and biocompatibility. This work indicated that the β-CD-Bi-CMCS NPs had the potentiality as promising nanocarriers for oral delivery of protein drugs.

Cyclodextrin metal–organic framework-based protein biocomposites

“…32 CD-MOFs are especially attractive for drug delivery systems as they display low toxicity compared to other MOF linkers. 33 CD-MOFs should be ideal for the storage and delivery of therapeutic proteins as cyclodextrin-protein complexes display sustained drug delivery, 34,35 reduced protein aggregation, and higher structural stability to physical perturbation. 36 In addition, cyclodextrins can induce the refolding of denatured proteins.…”