Ionic factors affecting motility, respiration and fertilization rate of the sperm of the bivalvePecten maximus (L.)

Faure, Catherine; Devauchelle, Nicole; Girard, Jean‐Pierre

doi:10.1007/bf00714581

Cited by 16 publications

(24 citation statements)

References 26 publications

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“…In contrast to Manila clam, sperm motility in Pacific oyster and Japanese scallop was immediately triggered in ASW, which is consistent with the findings of previous studies (Faure et al 1994, Dong et al 2002. In general, sperm motility was not fully activated, or immediately decreased, after activation in ASW (Fig.…”

Section: Discussionsupporting

confidence: 91%

“…However, [pH] e is not a key determinant to maintain sperm in the quiescent state in the testis because i) the [pH] e at which sperm motility was suppressed is much lower than the pH of testicular fluid (Faure et al 1994, Demoy-Schneider et al 2012 and ii) 5-HT induced sperm motility in Manila clam and Pacific oyster under acidic conditions, which is consistent with stimulation of germinal vesicle breakdown (GVBD) in the oocytes by 5-HT at similar pH ( Osanai 1985). In 5-HT containing NH 3 , sperm motility was triggered immediately (Fig.…”

Section: K7mentioning

confidence: 99%

“…Until now, inhibitory factors for sperm motility have been identified in mammals, fish, and invertebrates including sea urchins, ascidians, and starfish (Tosti 1994, Verma 2001. Despite the inhibitory effects of acidic pH on sperm motility in marine bivalve mollusks (Faure et al 1994, Dong et al 2002, Ohta et al 2007, Demoy-Schneider et al 2012, there is a significant lack of information to clarify the contribution of different ions. Sperm signaling in the initiation of motility mediated by pH, certain ions, or osmolality has been extensively studied in invertebrates and mammals (Darszon et al 1999, Inaba 2003, Yoshida et al 2008 and in fish (Alavi & Cosson 2005, Morisawa 2008.…”

Section: Introductionmentioning

confidence: 99%

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Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve

et al. 2014

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Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (Ruditapes philippinarum), Pacific oyster (Crassostrea gigas), and Japanese scallop (Patinopecten yessoensis). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5-7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K C /H C exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K C concentrations were two-to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing R40 mM K C or R2.5 mM 4-aminopyridine, suggesting K C efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca 2C is required for Ca 2C /calmodulin-dependent flagellar beating. Ca 2C influx occurs via Ca 2C channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca 2C channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca 2C rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca 2C channel blocker. Extracellular Na C is also essential for sperm motility initiation via regulation of Na C /Ca 2C exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K C , Ca 2C, and Na C .

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Section: Discussionsupporting

confidence: 91%

Section: K7mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve

et al. 2014

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“…In this species, spermatozoa released through the ducts, after intragonadal serotonin injection or thermal shock, can be activated when diluted in seawater, and simultaneously a high respiration rate is initiated. In contrast, a low motility, estimated using an arbitrary scale (from 1 immotile to 5 fully motile), was observed after the dilution of intratesticular spermatozoa into seawater, suggesting a sperm capacitation phenomenon occurring during gamete transit through the genital ducts (Faure et al 1994a). A decrease of the percentage of motile sperm was recorded two hours after sperm activation while no movement was observed after 4 h .…”

Section: Introductionmentioning

confidence: 89%

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Suquet

Quéré

Mingant

et al. 2013

Aquat. Living Resour.

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-Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (VAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, VAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and VAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 min incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher VAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, VAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, VAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 ± 7%, 162 ± 15 µm s −1 , 0.33 ± 0.12 and 212 ± 133 nmol ×10 −9 spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.

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“…Three sperm pools (collected from two males each) were used. Two different sperm-to-egg ratios were used: 50:1 and 500:1 (Faure et al 1994b). Triplicate controls were added (in which no sperm was added to eggs) to estimate the self-fertilization rate.…”

Section: Experimental Designmentioning

confidence: 99%

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

Suquet

Arsenault-Pernet

Ratiskol

et al. 2014

Aquat. Living Resour.

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-To develop selection programs for the great scallop, artificial reproduction of the species needs to be improved by gamete cryobanking. Here, a set of four experiments was designed in order to define the basic elements of a cryopreservation protocol for scallop sperm, including extender composition (experiment 1), cryoprotectant selection (2), cooling rate (3), and assessment of the effects of sperm cryopreservation on sperm motility and fertilization capacity (4). Sperm was collected after serotonin injection (100 µl of a 10-mM solution) and frozen in 500-µl straws. For the first three experiments, the percentage of motile fresh sperm (80 ± 4%, mean ± SEM) was significantly higher than that observed for thawed sperm (11 ± 2%). During the first experiment, no significant difference of the percentage of motile thawed sperm was observed among the three saline extenders tested: seawater, calcium-free Hanks' balanced salt solution (Ca-free HBSS) and DCSB4 solution. However, a complementary experiment (2) showed that a significantly higher percentage of motile thawed sperm was recorded using DCSB4 than with Ca-free HBSS as an extender. During the third experiment, sperm motility was higher when using polyethylene glycol (10% PEG) as a cryoprotectant, than when using dimethyl sulphoxide, DMSO (10 and 20%), methanol (10%) and ethylene glycol (10% EG). A higher D-larval rate was obtained during the fourth experiment, using fresh sperm than thawed sperm, and using a 500:1 sperm-to-egg ratio compared with a 50:1 ratio. There is some evidence of inter-individual variations in sperm tolerance to cryopreservation. In conclusion, the highest survival of great scallop sperm after thawing was recorded using the following conditions: DCSB4 extender (1:3 vol/vol sperm to extender dilution), PEG cryoprotectant (10%) and straws maintained at 5.5 cm above liquid nitrogen.

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Ionic factors affecting motility, respiration and fertilization rate of the sperm of the bivalvePecten maximus (L.)

Cited by 16 publications

References 26 publications

Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve

Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve

Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

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