Ionic and Histological Studies of Salivary Glands in Rats with Diabetes and Their Glycemic State After Laser Irradiation

Simões, Alyne; Oliveira, Elisabeth de; Campos, Luana; Nicolau, José Carlos

doi:10.1089/pho.2008.2452

Cited by 17 publications

(32 citation statements)

References 36 publications

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“…These anesthetic agents can increase blood glucose levels in control rats by α 2 ‐adrenergic receptors stimulation, also reducing fasting plasma insulin levels . This effect was also observed by Simões et al , controls had increased glycemia levels measured by glucometer, about 79.17% higher in controls without LPLI, and 97.62% higher in controls treated with LPLI (20 J/cm 2 ), on the day of sacrifice when compared to their diagnosis blood glucose values.…”

Section: Discussionmentioning

confidence: 99%

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Low‐power laser irradiation in salivary glands reduces glycemia in streptozotocin‐induced diabetic female rats

Fukuoka

Schröter

Nicolau

et al. 2016

Journal of Biophotonics

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Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory markers such as the high mobility group box 1 (HMGB1) protein. Thus this study aimed to analyze the LPLI effects upon blood glucose levels, plasma insulin and HMGB1 concentrations in a diabetes experimental rat model. Streptozotocin-induced diabetic rats were irradiated in the salivary glands area with a diode laser applied at 660 nm, 70 mW, 20 J/cm , 22.4 J, with a spot area of 0.028 cm and its effects were evaluated. LPLI significantly reduced diabetic rat hyperglycemia, without changing insulin or HMGB1 plasma levels, but possibly by ameliorating the insulin resistance in these animals. These findings suggest that LPLI might have a systemic effect, but more studies are necessary to better understand its mechanisms. Fasting blood glucose measured by peroxidase-glucose oxidase (PGO) method (A), showing a reduction of diabetic animals glycemia after LPLI. LPLI probably reduced the hyperglycemia in diabetes by improving the insulin resistance in these animals (B). C n = 10, CL n = 10, D n = 7 and DL n = 8. Data are expressed as mean ± SD; * P < 0.05 vs. respective control group; # P < 0.05 vs. D group.

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Section: Discussionmentioning

confidence: 99%

“…In diabetes, LPLI can improve the healing rate of diabetic wounds [18]. Our group previously reported that LPLI could reduce the hyper-glycemia in diabetic rats [19,20]. However, the biological molecular mechanism underlying the observed beneficial results remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Low‐power laser irradiation in salivary glands reduces glycemia in streptozotocin‐induced diabetic female rats

Fukuoka

Schröter

Nicolau

et al. 2016

Journal of Biophotonics

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“…22 Many different studies were conducted to investigate the effect of laser irradiation on parotid glands in diabetes rats. [23][24][25][26][27] Hyperglycemia in salivary glands occurred in diabetes rates. 24,26 LLLI could alter the activity of enzymes and also it effected on production of superoxide anions.…”

Section: Introductionmentioning

confidence: 99%

“…[23][24][25][26][27] Hyperglycemia in salivary glands occurred in diabetes rates. 24,26 LLLI could alter the activity of enzymes and also it effected on production of superoxide anions. 28 Simoes et al used infrared diode laser on submandibular and parotid glands and measured the protein concentration and enzymatic activities.…”

Section: Introductionmentioning

confidence: 99%

“…They concluded that, laser phototherapy modified the activity of catalase in diabetic rats compared to non-diabetic rats. 24 They showed that, LLLI could be used as an auxiliary therapy. 28 In another study, Campos et al used 780 nm diode laser on submandibular and sublingual rat glands, and claimed that, the activity of catalase and peroxidase was increased.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Low-Level Laser Irradiation on the Function of Glycated Catalase

et al. 2018

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Introduction: The aim of this work is to evaluate the effect of low-level laser irradiation (LLLI), by lasers with different wavelengths, on glycated catalase enzyme in vitro experimentally. Methods: This is done by measuring the activity and structure properties of glycated catalase enzyme. The structure properties were evaluated with circular dichroism (CD) and fluoroscopy methods. Three continuous wave (CW) lasers in the visible spectrum (λ = 450, 530, 638 nm) and a 100-ns pulsed laser in the infrared spectrum (λ = 905 nm) were chosen for comparison. For the infrared laser, same effects have been investigated for different energy doses. The effect of photon energy (hυ) at different wavelengths was measured on activity, CD, and fluoroscopy properties of catalase, and compared with the control group (samples without irradiation). The energy intensity of laser should not exceed 0.1 J/cm 2 . Experiments were performed on glycated catalase between 2 to 16 weeks after glycation of catalase. The LLLI effect was also investigated on the samples, by comparing the catalase activity, CD and fluoroscopy for different wavelengths. Results: Our results indicated, the decrease in catalase activity as a function of glycation time (weeks) for all samples, and a slight increase on its activity by different laser wavelengths irradiation for any fixed period of glycation time. Finally, the catalase activity has been increased as the laser's photon energy (hυ) intensified. More specifically, the blue laser (λ = 450 nm) had the most and the red laser (λ = 638 nm) had the least effect, and the green laser (λ = 530 nm) had the medium effect on catalase activity as well. Furthermore, pulsed laser had an additional effect by increasing energy dosage. Conclusion: As we expected in all experiments, an increase in the catalase activity was coincident with a decrease in the catalase fluoroscopy and CD parameters.

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Low Level Laser Therapy for hyposalivation and xerostomia

Simões

Campos

Arana-Chavez

et al. 2015

Lasers in Dentistry

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Ionic and Histological Studies of Salivary Glands in Rats with Diabetes and Their Glycemic State After Laser Irradiation

Abstract: LI should be explored as an auxiliary therapy for control of complications of diabetes because it can alter the carbohydrate and lipid metabolism of rats with diabetes.

Cited by 17 publications

References 36 publications

Low‐power laser irradiation in salivary glands reduces glycemia in streptozotocin‐induced diabetic female rats

Low‐power laser irradiation in salivary glands reduces glycemia in streptozotocin‐induced diabetic female rats

Effect of Low-Level Laser Irradiation on the Function of Glycated Catalase

Low Level Laser Therapy for hyposalivation and xerostomia

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