Ion transport across the early chick embryo: II. Characterization and pH sensitivity of the transembryonic short-circuit current

Abriel, Hugues; Katz, Uri; Kučera, Pavel

doi:10.1007/bf00238249

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…The CAM serves as a support for the extraembryonic respiratory capillaries, actively transports sodium and chloride from the allantoic sac and calcium from the eggshell into the embryonic vasculature, and forms part of the wall of the allantoic sac, which collects excretory products. Because of low cost, the simplicity of the surgical procedure, and the possibility to continuously observe the test site without disrupting it, the CAM is a common method for studying biological processes such as transport,1 gas exchanges,2 tumor transplant experiments,3 toxicity,4 and more recently angiogenesis 5–10. Typically, an opening is made in the shell to easily access and view the CAM.…”

Section: Introductionmentioning

confidence: 99%

The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials

Valdes

Kreutzer

Moussy

2002

J. Biomed. Mater. Res.

173

148

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Current in vivo models for testing biomaterials are time and labor intensive as well as expensive. This article describes a new approach for testing biomaterials in vivo using the chorioallantoic membrane (CAM) of the developing chicken embryo, as an alternative to the traditional mammalian models. Fertilized chicken eggs were incubated for 4 days, at which time a small window was cut in the shell of the egg. After 1 week of incubation, the CAM received several test materials, including the endotoxin LPS, a cotton thread and a Silastic tubing. One day and 1 week later, the tissue response to the test materials was assessed using gross, histological, and scanning electron microscope evaluations. The inflammatory response of the chorioallantoic membrane to biomaterials was fully characterized and found to be similar to that of the mammalian response and was also seen to vary according to test materials. We also found that the structure and geometry of the test materials greatly influenced the incorporation of the samples in the CAM. The similarity of the tissue response of the CAM with the mammalian models, plus the low cost, simplicity, and possibility to continuously visualize the test site through the shell window make this animal model particularly attractive for the rapid in vivo screening of biomaterials.

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Section: Introductionmentioning

confidence: 99%

The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials

Valdes

Kreutzer

Moussy

2002

J. Biomed. Mater. Res.

173

148

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“…Chloride ions and water move down the electroosmotic gradient and the saline solution floats to the top of the yolk because of its low density (Elias, 1964). Supporting this hypothesis are reports of unidirectional movement of sodium ions from the yolk sac across the blastoderm and early embryonic tissue (Abriel et al, 1994;Kučera et al, 1994;Stern & MacKenzie, 1983). Sodium transport by the blastoderm is electrogenic, sensitive to ouabain applied ventrally, and independent of extracellular chloride (Deeming et al, 1987;Kučera et al, 1994).…”

Section: Discussionmentioning

confidence: 96%

“…Sodium transport by the blastoderm is electrogenic, sensitive to ouabain applied ventrally, and independent of extracellular chloride (Deeming et al, 1987; Kučera et al, 1994). The active sodium transport drives glucose and fluid into the intraembryonic space, across and around the blastoderm which, in the absence of blood circulation, could allow the renewal of extracellular fluid and disposal of wastes and thus maintain cell homeostasis (Abriel et al, 1994). Babiker and Baggott (1995) concluded that secretion of subembryonic fluid is dependent upon active transport of sodium across the blastoderm; an amiloride‐sensitive Na + /H + exchanger and CO 2 hydration catalyzed by carbonic anhydrase also appear to be involved.…”

Section: Discussionmentioning

confidence: 99%

Shell‐less culture system for chick embryos from the blastoderm stage to hatching

Dunn

2023

J Exp Zool Pt A

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Since 2014, methods have been described to hatch chick embryos from shell‐less culture after egg contents are first incubated within shells for 55–70 h. The present report describes for the first time a shell‐less culture system for chick embryos from the blastoderm stage to hatching. For the first 69–70 h, egg contents suspended in polymethylpentene kitchen wrap (F.O.R. Wrap, Riken Fabro, Tokyo, Japan) supported in 6.35 or 6.67 cm inside diameter tripods and covered with a disc of immobilized Milli‐Wrap, were rotated back and forth through 90° at 16 or 22 cycles per minute (CPM). Subsequently, the Milli‐Wrap disc was removed and culture tripods were transferred to environmental chambers, which were rocked ±20° through incubation day 8.5 (E8.5). From E9, environmental chambers were maintained in the horizontal position through to hatching with controlled O2 and CO2. To provide supplemental calcium, an aqueous solution containing 100 mg/mL of calcium l‐lactate hydrate was injected through the plastic wrap into the albumen at E9 (2.5 mL) and at E13 (1.0 mL) or E15 (1.0 mL). After incubation for 69–70 h at 16 or 22 CPM, 80%–83% of previously unincubated egg contents yielded apparently normal embryos. Hatch rate of normal embryos resulting from turntable incubation at 16 or 22 CPM was approximately 43%. Of note, egg contents remained in the same culture tripod from blastoderm stage to hatching. This technique may find use as an educational tool and in basic investigations of early embryogenesis, teratogenesis, and gene transfer experiments.

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“…37 Types of epithelium involved in ion transport and current, such as renal and intestinal epithelium Epithelial tissue plays a critical role in ion transport and current across various organ systems in the body. 38 Two types of epithelium that are particularly involved in this process are renal epithelium and intestinal epithelium. Renal epithelium lines the walls of the nephron, the functional unit of the kidney, and is responsible for regulating the levels of electrolytes and water in the body.…”

Section: Definition Of Epitheliummentioning

confidence: 99%

Ion Transport and Current are Linked to Membrane Proteins and Epithelium Transport

Kang

2024

Preprint

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Membrane proteins play a significant role in ion transport across cell membranes. They act as channels or transporters that allow ions to move across the membrane. Channels are typically selective for specific ions, while transporters move multiple types of ions. These proteins use energy from ATP or the electrochemical gradient to move ions against their concentration gradient. The movement of ions through these proteins generates an electrical potential difference across the membrane, which is important for various physiological processes. Noticeably, the epithelial tissues form barriers that separate different compartments in the body, such as the lumen of the gut, the ducts of glands, and the external environment. Epithelial cells play a significant role in ion transport and current in biological systems. For example, epithelial cells in the gut are responsible for the absorption of nutrients and water, and they use ion channels and transporters to move ions such as sodium, potassium, and chloride across their membranes. This process creates an electrical potential difference across the epithelial cell layer, leading to the generation of a current that drives ion movement. Importantly, the disruptions in membrane proteins and epithelial transport are implicated in many diseases, including cystic fibrosis, hypertension, arrhythmia, kidney disease, diarrhea, and renal failure. Studying the mechanisms will identify potential therapeutic targets and develop treatments for these diseases.

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Ion transport across the early chick embryo: II. Characterization and pH sensitivity of the transembryonic short-circuit current

Cited by 8 publications

References 32 publications

The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials

The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials

Shell‐less culture system for chick embryos from the blastoderm stage to hatching

Ion Transport and Current are Linked to Membrane Proteins and Epithelium Transport

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