Ion selective electrode (in-line analyzer) versus UV-spectroscopy (at-line analyzer); which strategy offers more opportunities for real time monitoring of the degradation kinetics of pyridostigmine bromide

El-Rahman, Mohamed K. Abd; Salem, Maissa Y.

doi:10.1016/j.snb.2015.05.092

Cited by 20 publications

(8 citation statements)

References 15 publications

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“…In order to investigate the hydrolysis and subsequent deacetylation behavior of satraplatin in solution we have conducted potentiometric experiments on several aqueous satraplatin solutions at pH values ranging from 6.5 to 7.0. Employing ion selective potentiometric sensors offers some outstanding advantages such as their inherent capability of providing a continuous signal for real-time reaction monitoring, in addition to their unique ability to provide a rapid route for simultaneous determination of ionic species, making this technique increasingly prominent in studies of degradation kinetics of easily hydrolyzable ionizable/ionic drugs. − Figure shows the time trace of the potentiometric response for the two sensors as the concentration of satraplatin increased from 5 μM to 1 mM.…”

Section: Results and Discussionsupporting

confidence: 86%

Hydrolysis in Acidic Environment and Degradation of Satraplatin: A Joint Experimental and Theoretical Investigation

et al. 2017

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For the synthesis and selection of active platinum-based anticancer drugs that perform better than cisplatin and its analogues, six-coordinate octahedral Pt(IV) complexes appear to be promising candidates as, being kinetically more inert and more resistant to ligand substitution than four-coordinate Pt(II) centers, they are able to minimize unwanted side reactions with biomolecules prior to DNA binding. Due to their kinetic inertness, Pt(IV) complexes have also been exploited to bypass inconvenient intravenous administration. The most prominent example is satraplatin (Sat.) which is the first platinum antineoplastic agent reported to have oral activity. The present paper deals with a theoretical DFT investigation of the influence that the acidity of the biological environment can have on the activity of satraplatin and analogous octahedral Pt(IV) complexes having two carboxylates as axial ligands. Moreover, here the outcomes of a joint electrospray ionization mass spectrometry and DFT investigation of the fragmentation pathways of the protonated satraplatin are reported. Calculations show that the simulated acidic environment has an important impact on the satraplatin reactivity causing a significant lowering of the barrier that is necessary to overcome for the hydrolysis of the first acetate ligand to occur. Data from electrospray ionization mass spectrometry, H NMR, and potentiometric experiments strongly suggest that the loss of CHCOOH from the protonated satraplatin ion [Sat. + H] takes place almost immediately upon dissolution of satraplatin in methanol-water, DO, and water solutions, respectively, at room temperature.

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Section: Results and Discussionsupporting

confidence: 86%

Hydrolysis in Acidic Environment and Degradation of Satraplatin: A Joint Experimental and Theoretical Investigation

et al. 2017

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“…To this end, we have recently introduced the first generic approach for in-line monitoring of the degradation kinetics of organic compounds by exploiting the outstanding advantages offered by these potentiometric sensors as a proof-of-concept . Subsequently, we have demonstrated the opportunities offered by ISEs in reference to at-line monitoring (UV spectrophotometry) for continuous tracking of the degradation kinetics of pyridostigmine bromide . Building on these works and in light of GAC principles, this paper intends to further investigates the opportunities offered by ISEs with respect to off-line techniques (HPLC) for continues tracking of the degradation kinetics of easily hydrolyzable ionic drugs.…”

Section: Resultsmentioning

confidence: 99%

“…The kinetics studies were performed following the procedures described in our previous work, , and it will be briefly represented in the following;…”

Section: Experimental Sectionmentioning

confidence: 99%

Just-Dip-It (Potentiometric Ion-Selective Electrode): An Innovative Way of Greening Analytical Chemistry

El-Rahman

Zaazaa

ElDin

et al. 2016

ACS Sustainable Chem. Eng.

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“Green analytical chemistry” (GAC) is a vital area towards the concept of sustainability. As a consequence of the widespread application of HPLC in drug-related analytical investigations and the resulting contamination of the environment with organic solvents questions have been raised about the toxicity/greenness of HPLC in the ecosystem. Traditional analytical separation technologies yield approximately 50 mL of waste per analytical data point. To this end, the pharmaceutical community continues to search for greener opportunities to markedly reduce the amount of organic waste produced and move from conventional offline separation based methodologies to greener in-line alternatives. In this contribution, we’re adopting a “Just-Dip-It” approach with the ultimate goal of advancing and exploiting the potentiometric sensors to their most effective use in different disciplines of drug development. The unique abilities of these ion-selective electrodes (ISEs) for in-line measurements is the key driver for adoption of GAC principles to improve environmental friendliness of the analytical methods. For a meaningful comparison, this work compares the organic waste resulting from ISEs versus HPLC for degradation kinetics monitoring of active pharmaceutical ingredients (APIs) with respect to the 12 principles of GAC. Ipratropium bromide (IP) was chosen as a hydrolyzable anticholinergic drug, and its degradation kinetics were monitored by the two techniques. The first in-line strategy is attained by dipping a highly integrated IP membrane sensor for continuous monitoring of the hydrolysis kinetics of IP by tracing the emf decline over the time scale. The second off-line strategy utilizes a separation-based chromatographic HPLC method via discontinuous tracking the decrease of IP peak area spectroscopically at 220 nm over time. The advantages and shortcomings of each strategy considering GAC principles are highlighted. The merits of these benign real-time analyzers (ISEs) that can deliver equivalent analytical results as HPLC while significantly reducing solvent consumption/waste generation are described. Finally, an applicable strategy for expansion of the Just-Dip-It approach to different disciplines of drug-related analytical investigations is addressed.

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“…The kinetics study was done using a previously reported procedure [34,35].The cell design consists of a Cu microfabricated electrode and a reference electrode that are immersed into the NEO solution and connected to the two terminals of a potentiometer. The alkaline hydrolysis of NEO was monitored by continuous measuring the decrease in potential over time.…”

Section: Reaction Ordermentioning

confidence: 99%

Design of Copper Microfabricated Potentiometric Sensor for in‐line Monitoring of Neostigmine Degradation Kinetics

et al. 2021

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The quality of pharmaceutical products is critical for human health. Drug development requires tools to assess the presence of degradation products and contaminants during the manufacturing and storage processes. Accelerated stress degradation and kinetic studies play a vital role in predicting final product stability. This work describes the design of potentiometric sensor based on copper microfabricated electrodes for in‐line tracking the degradation kinetics of neostigmine. The proposed electrochemical technique provides a continuous profile for the hydrolysis of NEO under different temperatures and pH. The hydrolysis activation energy was found to be 18.88 kcal mol−1 which was aligned with the reported hydrolysable ester values. Consequently, the kinetic data analysis is crucial to predict the optimum analysis and storage conditions.

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Ion selective electrode (in-line analyzer) versus UV-spectroscopy (at-line analyzer); which strategy offers more opportunities for real time monitoring of the degradation kinetics of pyridostigmine bromide

Cited by 20 publications

References 15 publications

Hydrolysis in Acidic Environment and Degradation of Satraplatin: A Joint Experimental and Theoretical Investigation

Hydrolysis in Acidic Environment and Degradation of Satraplatin: A Joint Experimental and Theoretical Investigation

Just-Dip-It (Potentiometric Ion-Selective Electrode): An Innovative Way of Greening Analytical Chemistry

Design of Copper Microfabricated Potentiometric Sensor for in‐line Monitoring of Neostigmine Degradation Kinetics

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