Ion-selective electrode for the determination of some multidrug resistance reversers

Ortuño, J; Hernández, Jorge; Sánchez‐Pedreño, Concepción

doi:10.1016/j.snb.2005.12.021

Cited by 33 publications

(9 citation statements)

References 21 publications

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“…The polished electrode surface was coated with the active membrane by dipping the exposed end into the coating solution that was described under (2.5. membrane composition), ten times and allowing the film left on graphite rod to dry in air for 1 min each time. The prepared electrode was preconditioned by soaking for 6 h in 1.0 x 10 -3 mol L -1 SIM solution [16]. All potentiometric measurements were performed using the following cell assembly: Graphite rode / membrane / test solution // KCl salt bridge // SCE.…”

Section: Coated Graphite Electrodementioning

confidence: 99%

Electrochemical Sensors for Direct Determination of Simvastatin in Pharmaceutical Formulations and Biological Fluids

Alarfaj

Aly

El‐Tohamy

2012

J. Chil. Chem. Soc.

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The construction and performance characteristics of simvastatin (SIM) selective electrodes were developed. Three types of electrodes: plastic membrane I, coated wire II, and coated graphite rod III were constructed based on the incorporation of simvastatin with phosphotungstic (PTA) or phosphomolybdic (PMA) acids and mixed ion pair (PTA/PMA) for the three electrodes, respectively. The influence of membrane composition, kind of plasticizer, type of ion-pair, pH of the test solution, soaking time, and foreign ions on the electrodes was investigated. The electrodes showed a Nernstain response with a mean calibration graph slope of 56.24±0.43, 55.44±0.14 and 58.93±0.34 mV decade -1 at 25ºC over simvastatin concentration range from 1.0 x 10 -6 -5.0 x 10 -2 , 9.0 x 10 -6 -5.0 x 10 -3 and 9.0 x 10 -7 -1.0 x 10 -2 mol L -1 , with detection limit of 5.0 x 10 -7 , 3.9 x 10 -6 and 3.2 x 10 -7 mol L -1 for electrode I, II and III, respectively. The pH range for the proposed electrodes was 4-7. The influence of possible interfering species such as common inorganic cations, many sugars, amino acids and a pharmacologically related drug 'ezetimibe' was studied. Statistical student's t-test and F test showed insignificant systematic error between proposed and official methods.

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Section: Coated Graphite Electrodementioning

confidence: 99%

Electrochemical Sensors for Direct Determination of Simvastatin in Pharmaceutical Formulations and Biological Fluids

Alarfaj

Aly

El‐Tohamy

2012

J. Chil. Chem. Soc.

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“…It depends on non-aqueous potentiometric titrimetry or spectrophotometry in the UV region [7,8]. Different analytical methods and techniques have been developed for Cpn determination, including chromatographic [9][10][11], potentiometric [12][13][14][15], voltammetric [16,17], fluorimetric [18][19][20], and UV-visible spectrophotometric [21][22][23][24] techniques. Quantitative analysis, using UV-visible absorption spectroscopy, is one of the most widely used methods [25,26].…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation of Assay of Chlorpromazine Hydrochloride Tablet Formulation Using Ultra Violet Visible Spectrophotometry

Shatti¹

2014

J Anal Bioanal Tech

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The International Conference on Harmonisation [Q2 (R1)] guidelines was applied for this one-step spectrophotometric estimation of chlorpromazine hydrochloride in neurazine tablet formulation. The method was found to be simple, safe, sensitive, and validated for the assay of chlorpromazine hydrochloride using bromophenol blue, citrate buffer pH 3, and water as diluents. It was also found to be an accurate, reproducible, and cost-effective quality-control tool for the routine analysis of chlorpromazine hydrochloride in standard and pharmaceutical forms. Method Development and Validation of Assay of Chlorpromazine Hydrochloride Tablet Formulation Using Ultra Violet Visible SpectrophotometryLaila A Al Shatti* Diluent preparationDistilled water was used as a diluent. Standard preparationA bromophenol blue (1×10 -3 M) and Cpn-HCl (1×10 -3 M) stock solution was prepared to be used for the preparation of standard solutions. In 100 mL volumetric flasks, 15 mL BrPB, 3 mL citrate buffer (pH 3), and different aliquots of Cpn-HCl (1×10 -3 M) were added to prepare five standard solutions of Cpn-HCl ranging in concentration from 2.553-16 ppm. The five standard solutions were scanned for the maximum wavelength (nm) previously assigned, and a calibration curve was set to perform the linearity test. Pharmaceutical test preparationTen tablets of neurazine formulation were ground and weighed (4.4870 g). Each tablet contained 100 mg active ingredient of Cpn-HCl. To prepare the stock solution (1×10 -3 ) of the formulation, 0.1594 g of the ground tablets were weighed and dissolved with distilled water in a 100 mL volumetric flask. The stock solution was diluted to prepare (1×10 -4 M). A test solution was made by adding 15 mL bromophenol blue, 3 mL citrate buffer (pH 3), and 10 mL neurazine (1×10 -4 M) (ppm). The solution was tested by the extrapolation method to determine the concentration of the neurazine test sample. InstrumentationA UV-visible double beam spectrophotometer with matched quartz cell (1 cm) (model no. Evolution 201) by Thermo Scientific, 81 Wyman Street Waltham, Massachusetts, US was used. Results and Discussion Development and optimization of the spectrophotometric methodThe selection of the proper wavelength in this method depends on the sample, diluent, and test solution. Spectrophotometric quantitative determination of chlorpromazine needs the development of rugged and suitable conditions after testing different parameters, such as diluents, buffer, buffer concentration, and other chromatographic conditions. Different trials with varied compositions of diluents and buffer should be conducted to choose the optimum conditions. Selection of wavelengthThe standard solution and the pharmaceutical test forms were scanned by UV-visible spectrophotometer between 200 and 700 nm on spectrum mode before and after using BrPB (Figures 1 and 2). Both spectra showed that Cpn-HCl has a maximum wavelength λ max at 314. Bromophenol blue was scanned to avoid the interferences of wavelengths (Figure 3). The spectrum pf BrPB ...

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“…Ion exchangers and neutral macrocyclic compounds have been employed over the past decades for this purpose. To our knowledge, the only two CPZ selective electrodes reported in literature [43,44] employed ion-exchangers as sensing materials.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic sensors of molecularly-imprinted polymers for chlorpromazine determination

Moreira¹,

Sales²

2011

Materials Science and Engineering: C

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A new man-tailored biomimetic sensor for Chlorpromazine host-guest interactions and potentiometric transduction is presented. The artificial host was imprinted within methacrylic acid, 2-vinyl pyridine and 2-acrylamido-2-methyl-1-propanesulfonic acid based polymers. Molecularly imprinted particles were dispersed in 2-nitrophenyloctyl ether and entrapped in a poly(vinyl chloride) matrix. Slopes and detection limits ranged 51-67 mV/decade and 0.46-3.9 μg/mL, respectively, in steady state conditions. Sensors were independent from the pH of test solutions within 2.0-5.5. Good selectivity was observed towards oxytetracycline, doxytetracycline, ciprofloxacin, enrofloxacin, nalidixic acid, sulfadiazine, trimethoprim, glycine, hydroxylamine, cysteine and creatinine. Analytical features in flowing media were evaluated on a double-channel manifold, with a carrier solution of 5.0 × 10 −2 mol/L phosphate buffer. Near-Nernstian response was observed over the concentration range 1.0 × 10 −4 to 1.0 × 10 −2 mol/L. Average slopes were about 48 mV/decade. The sensors were successfully applied to field monitoring of CPZ in fish samples, offering the advantages of simplicity, accuracy, automation feasibility and applicability to complex samples.

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Ion-selective electrode for the determination of some multidrug resistance reversers

Cited by 33 publications

References 21 publications

Electrochemical Sensors for Direct Determination of Simvastatin in Pharmaceutical Formulations and Biological Fluids

Electrochemical Sensors for Direct Determination of Simvastatin in Pharmaceutical Formulations and Biological Fluids

Method Development and Validation of Assay of Chlorpromazine Hydrochloride Tablet Formulation Using Ultra Violet Visible Spectrophotometry

Biomimetic sensors of molecularly-imprinted polymers for chlorpromazine determination

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