Ion-pair reversed-phase high-performance liquid chromatography analysis of oligonucleotides:

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

“…34 In other words, the addition of C or G does not increase oligonucleotide retention as dramatically as the addition of A, and particularly T, because of the hydrophobicity of C and of G are similar. 9 In present study, on the IB column, little change in the retention times with temperature was observed for the oligonucleotides examined at pH 4.5. At pH 3.0, the retention times were slightly retarded with increasing temperature.…”

Analysis of oligonucleotides by liquid chromatography with alkylamide stationary phase

“…There are many theoretical studies concerning the interaction of oligonucleotides within the surface of stationary phase [8][9][10]. Most of them include complex mechanisms that comprise different interactions in hydro-organic solvents [1][2][3][8][9][10].…”

“…Utilization of this packing material does not achieve high efficiency and selectivity in the case of the studied compounds [8][9][10]. Thus, another type of stationary phase was chosen for the present study, namely alkylamide.…”

A New Combination Strategy as Applied in Predicting Chromatographic Retention Times of Oligonucleotides at a Range of Temperatures from 30 °C to 80 °C

“…Most importantly, the present representation method is derived from 1209 0D-3D parameters; therefore, it has strong characterization ability for structures of oligonucleotides. Gilar et al 15 developed a model for the retention prediction of oligonucleotides in ion-pair reversed-phase chromatography using input structural parameters from length and base composition; however, the model possessed limited application and was not appropriate for relatively low temperature conditions where the secondary structure is not suppressed by thermal denaturing. For the current method, the QSRR models can be successfully used to predict the retention time of oligonucleotides at a wide range of temperatures from 30°C to 80°C .…”

“…Although much success has been achieved in the retention prediction of drug molecules and peptides by QSRR models, there are few applications in quantitative chromatographic retention predictions of oligonucleotides. Gilar et al 15 used input structural parameters from length and base composition of oligonucleotides to develop a model for the retention prediction of oligonucleotides in ion-pair reversed-phase chromatography. Kohlbacher et al 16 developed models by support vector regression using the information of length, sequence and predicted secondary structures of oligonucleotides.…”