Ion-pair HPLC method for the quantification of metformin in human urine

Troja, Eva; Deda, Leonard; Boçari, Gëzim

doi:10.17145/jab.16.004

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…The extraction of the sample is quite complicated and time taking because of the polar nature of MET. The run time of the prevailing methods was greater than 9 min while our developed method has a shorter runtime of eight minutes [26]. Even the injection quantity of the sample became higher in most of the methods.…”

Section: Discussionmentioning

confidence: 92%

Quantitation of Metformin in Urine by Rp-HPLC Method and Its Application in Pharmacokinetics

Rebecca

Sudha

Vijayakumar

et al. 2021

Int J Pharm Pharm Sci

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Objective: To develop and validate an easy and sensitive HPLC method for quantitation of metformin in urine. Methods: The technique involved deproteinisation of urine sample with methanol and analysis of the supernatant the usage of Zorbax 300–SCX, 4.6 X 150 mm ID, 5 µm particle size and UV detection at a wavelength of 233 nm. Results: The assay was specific for metformin and linear from 1.25 to 50.0μg/ml. The relative standard deviation of intra-and inter-day assays was lesser than 7%. The recovery of metformin from urine ranged from 97-103%. Conclusion: An easy and sensitive HPLC approach for quantitation of metformin in urine had been developed. Due to its simplicity in sample preparation and instrumentation, this technique can be used for pharmacokinetic studies of metformin in urine samples.

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Section: Discussionmentioning

confidence: 92%

Quantitation of Metformin in Urine by Rp-HPLC Method and Its Application in Pharmacokinetics

Rebecca

Sudha

Vijayakumar

et al. 2021

Int J Pharm Pharm Sci

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Urinary excretion of metformin in diabetic patients with and without tuberculosis

Sudha

Bharathiraja

et al. 2023

Indian Journal of Tuberculosis

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Development and validation of a new analytical HPLC method for simultaneous determination of the antidiabetic drugs, metformin and gliclazide

Gedawy

Al‐Salami

Dass

2019

Journal of Food and Drug Analysis

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An efficient and simple HPLC method has been developed and validated for the simultaneous determination of gliclazide and metformin hydrochloride in bulk and was applied on marketed metformin and gliclazide products. The mobile phase used for the chromatographic runs consisted of 20 mM ammonium formate buffer (pH 3.5) and acetonitrile (45:55, v/v) The separation was achieved on an Alltima CN (250 mm × 4.6 mm x 5μ) column using isocratic mode. Drug peaks were well separated and were detected by a UV detector at 227 nm. The method was linear at the concentration range 1.25–150 μg/ml for gliclazide and 2.5–150 μg/ml for metformin respectively. The method has been validated according to ICH guidelines with respect to system suitability, specificity, precision, accuracy and robustness. Metformin limit of detection (LOD) and limit of quantification (LOQ) were 0.8 μg/ml and 2.45 μg/ml respectively while LOD and LOQ for gliclazide were 0.97 μg/ml and 2.95 μg/ml respectively.

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Ion-pair HPLC method for the quantification of metformin in human urine

Cited by 5 publications

References 23 publications

Quantitation of Metformin in Urine by Rp-HPLC Method and Its Application in Pharmacokinetics

Quantitation of Metformin in Urine by Rp-HPLC Method and Its Application in Pharmacokinetics

Urinary excretion of metformin in diabetic patients with and without tuberculosis

Development and validation of a new analytical HPLC method for simultaneous determination of the antidiabetic drugs, metformin and gliclazide

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