Ion pair chromatography on reversed-phase layers

Volkmann, D.

doi:10.1007/bfb0024398

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…An additional RP method of analysis based on ion‐pair RP‐HPLC (Volkmann, ) is also being used currently. Ion‐paired reversed‐phase chromatography has been described as “a versatile platform for the analysis of RNA” by Dickman ().…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analysis and Purification of Synthetic Nucleic Acids Using HPLC

Sinha¹

2015

CP Nucleic Acid Chemistry

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Chromatographic methods have been essential tools for analysis and purification of synthetic oligonucleotides since the 1970s. Significant developments in terms of instruments and stationary phases (media) have been made during the past several decades; among the latest are sub-micron to micron particles for the media, as well as ultra performance liquid chromatography (UPLC). Micron and sub-micron particles have increased product resolution. Applications of recently developed methods such as IP-RP-HPLC and LC-MS have been discussed for analysis, along with use of various methods for purification. Utilization of UPLC has decreased analysis time, increasing the throughput for analysis. Commonly used methods for analysis and purification of synthetic oligonucleotides have been described in this unit.

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Section: Chromatographic Methodsmentioning

confidence: 99%