Ion Optical Evaluation of a Miniature Double-Focusing Mass Spectrograph

Nishiguchi, Masaru; Ishihara, Morio; Katakuse, Itsuo; Toyoda, Michisato

doi:10.1255/ejms.907

Cited by 7 publications

(1 citation statement)

References 20 publications

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“…One mass spectrometry technology that might be competitive with TOF is magnetic sector [68][69][70] with array detection technology. With an atomic mass spectrometry history of nearly a decade, multicollector mass spectrometry 71 offers the potential to measure up to 9 mass channels simultaneously; indeed, the instrumentation has found important use in isotope ratio analysis.…”

Section: Single-cell Dna and Rna Sequencingmentioning

confidence: 99%

From single cells to deep phenotypes in cancer

2012

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In recent years, major advances in single-cell measurement systems have included the introduction of high-throughput versions of traditional flow cytometry that are now capable of measuring intracellular network activity, the emergence of isotope labels that can enable the tracking of a greater variety of cell markers and the development of super-resolution microscopy techniques that allow measurement of RNA expression in single living cells. These technologies will facilitate our capacity to catalog and bring order to the inherent diversity present in cancer cell populations. Alongside these developments, new computational approaches that mine deep data sets are facilitating the visualization of the shape of the data and enabling the extraction of meaningful outputs. These applications have the potential to reveal new insights into cancer biology at the intersections of stem cell function, tumor-initiating cells and multilineage tumor development. In the clinic, they may also prove important not only in the development of new diagnostic modalities but also in understanding how the emergence of tumor cell clones harboring different sets of mutations predispose patients to relapse or disease progression.

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Section: Single-cell Dna and Rna Sequencingmentioning

confidence: 99%

From single cells to deep phenotypes in cancer

2012

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Current literature in mass spectrometry

2008

J. Mass Spectrom.

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WJ, De Voe DL, Lee CS. Mass spectrometry-based tissue proteomics for cancer biomarker discovery. Pers Med 2007; 4: 45. Bohme DK. Gaseous ions and chemical mass spectrometry. Can J Chem 2008; 86: 177. Bollen G. Ion trap mass spectrometry of rare isotopes. Acta Phys Pol B 2008; 39: 433. Boonen K, Landuyt B, Baggerman G, Husson SJ, Huybrechts J, Schoofs L. Peptidomics: The integrated approach of MS, hyphenated techniques and bioinformatics for neuropeptide analysis. J Sep Sci 2008; 31: 427. Brouwers EEM, Tibben M, Rosing H, Schellens JHM, Beijnen JH. The application of inductively coupled plasma mass spectrometry in clinical pharmacological oncology research. Mass Spectrom Rev 2008; 27: 67. Harvey DJ. Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 2001-2002. Mass Spectrom Rev 2008; 27: 125. Hou XL, Roos P. Critical comparison of radiometric and mass spectrometric methods for the determination of radionuclides in environmental, biological and nuclear waste samples. Anal Chim Acta 2008; 608: 105. Larsson L. Use of mass spectrometry for determining microbial toxins in indoor environments. J Environ Monit 2008; 10: 301. Mondello L, Tranchida PQ, Dugo P, Dugo G. Comprehensive two-dimensional gas chromatography-mass spectrometry: A review. Mass Spectrom Rev 2008; 27: 101. Mueller LN, Brusniak MY, Mani DR, Aebersold R. An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data. J Proteome Res 2008; 7: 51. . ISOLTRAP: An on-line Penning trap for mass spectrometry on short-lived nuclides. Eur Phys J A 2008; 35: 1. Sutherland BW, Toews J, Kast J. Special feature: Perspective -Utility of formaldehyde cross-linking and mass spectrometry in the study of protein-protein interactions. J Mass Spectrom 2008; 43: 699.

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IonCCD Detector for Miniature Sector-Field Mass Spectrometer: Investigation of Peak Shape and Detector Surface Artifacts Induced by keV Ion Detection

Hadjar

Schlathölter

Davila

et al. 2011

J. Am. Soc. Mass Spectrom.

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A recently described ion charge coupled device detector IonCCD (Sinha and Wadsworth, Rev. Sci. Instrum. 76(2), 2005; Hadjar, J. Am. Soc. Mass Spectrom. 22(4), [612][613][614][615][616][617][618][619][620][621][622][623][624] 2011) is implemented in a miniature mass spectrometer of sector-field instrument type and MattauchHerzog (MH)-geometry (Rev. Sci. Instrum. 62(11), 2618-2620, 1991; Burgoyne, Hieftje and Hites J. Am. Soc. Mass Spectrom. 8(4), 307-318, 1997; Nishiguchi, Eur. J. Mass Spectrom. 14 (1), 7-15, 2008) for simultaneous ion detection. In this article, we present first experimental evidence for the signature of energy loss the detected ion experiences in the detector material. The two energy loss processes involved at keV ion kinetic energies are electronic and nuclear stopping. Nuclear stopping is related to surface modification and thus damage of the IonCCD detector material. By application of the surface characterization techniques atomic force microscopy (AFM) and X-ray photoelectrons spectroscopy (XPS), we could show that the detector performance remains unaffected by ion impact for the parameter range observed in this study. Secondary electron emission from the (detector) surface is a feature typically related to electronic stopping. We show experimentally that the properties of the MH-mass spectrometer used in the experiments, in combination with the IonCCD, are ideally suited for observation of these stopping related secondary electrons, which manifest in reproducible artifacts in the mass spectra. The magnitude of the artifacts is found to increase linearly as a function of detected ion velocity. The experimental findings are in agreement with detailed modeling of the ion trajectories in the mass spectrometer. By comparison of experiment and simulation, we show that a detector bias retarding the ions or an increase of the B-field of the IonCCD can efficiently suppress the artifact, which is necessary for quantitative mass spectrometry.

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Ion Optical Evaluation of a Miniature Double-Focusing Mass Spectrograph

Cited by 7 publications

References 20 publications

From single cells to deep phenotypes in cancer

From single cells to deep phenotypes in cancer

Current literature in mass spectrometry

IonCCD Detector for Miniature Sector-Field Mass Spectrometer: Investigation of Peak Shape and Detector Surface Artifacts Induced by keV Ion Detection

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