Ion Mobility Spectrometry

Hill, Herbert H.; Siems, William F.; Louis, Robert H. St.; McMinn, Dennis G.

doi:10.1021/ac00222a716

Cited by 253 publications

(149 citation statements)

References 21 publications

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“…5,6 IMS is based on spatial separation of gas-phase ion species due to differences in their mobilities, analogous to capillary electrophoresis (CE) in the condensed phase.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Ion Mobility Spectrometry-Orthogonal Time-of-Flight Mass Spectrometry

et al. 2007

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Ion mobility spectrometry (IMS) coupled to orthogonal time-of-flight mass spectrometry (oTOF) has shown significant promise for the characterization of complex biological mixtures. The enormous complexity of biological samples (e.g. from proteomics) and the need for both biological and technical analysis replicates imposes major challenges for multidimensional separation platforms in regard to both sensitivity and sample throughput. A major attraction of the IMS-TOF MS platform is separation speeds exceeding that of conventional condensed-phase separations by orders of magnitude. Known limitations of the IMS-TOF MS platforms include the need for extensive signal averaging due to factors that include significant ion losses in the IMS-TOF interface and an ion utilization efficiency of less than ~1% with continuous ion sources (e.g. ESI). We have developed a new multiplexed ESI-IMS-TOF mass spectrometer that enables lossless ion transmission through the IMS-TOF as well as a utilization efficiency of >50% for ions from the ESI source. Initial results with a mixture of peptides show a ~10-fold increase in signalto-noise ratio with the multiplexed approach compared to a signal averaging approach, with no reduction in either IMS or TOF MS resolution.

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“…5,6 IMS is based on spatial separation of gas-phase ion species due to differences in their mobilities, analogous to capillary electrophoresis (CE) in the condensed phase.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Ion Mobility Spectrometry-Orthogonal Time-of-Flight Mass Spectrometry

et al. 2007

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“…The technique of IMS has primarily been used for the characterization of small molecules by correlating diffusion rate with size and shape [13,14]. Ion mobilities are measured by determining the time required for a pulse of ions to traverse a uniform electric field in the presence of a buffer gas [15,16] and the size of the particle is directly related to its mobility.…”

Section: Introductionmentioning

confidence: 99%

Electrospray ion mobility spectrometry of intact viruses

Thomas

Bothner

Traina³

et al. 2004

Journal of Spectroscopy

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Abstract. Characterizing supramolecular interactions offers significant challenges using NMR or crystallographic techniques either because of size limitations or the difficulty in forming suitable crystals, while mass spectrometry is largely limited to low resolution mass information. Here we report gas phase measurements of intact virus particles using electrospray ion mobility spectrometry with an accuracy in radial measurements that were sufficient to differentiate closely related species. In addition, measured diameters indicate that iscosahedral virus particles retain their structure in the gas phase as well as undergoing a slight compaction in the absence of solvent. Analysis of the human pathogen adenovirus represents the largest and most sophisticated biomolecular complex detected in the gas phase to date. These results, on a diverse set of viral systems, suggest that ion mobility spectrometry may have broad applications for the analysis of biological complexes.

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“…It has been used to detect drugs, chemical warfare agents, explosives and environmental pollutants. 5,6 Several groups have used mobility measurements to characterize the size distribution of aerosol particles and small metal particles. 7,8 In addition to information about ion mobilities, drift tube studies can provide information about ionÈmolecule reaction kinetics and equilibria.…”

Section: Introductionmentioning

confidence: 99%