Ion Mobility–Mass Spectrometry

McLean, John A.; Schultz, J.; Woods, Amina S.

doi:10.1002/9780470588901.ch12

Cited by 14 publications

(13 citation statements)

References 129 publications

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“…Classically, this was achieved by varying L , 34,35 however, it is more straightforward and accurate to vary the electric field used for ion mobility dispersion, which is referred to here as a stepped field method. 14 The choice of electric fields used in the time correction will affect both the magnitude and precision of the resulting CCS since at low electric fields the ion mobility distribution is spread across a broad range of times, whereas high electric fields result in narrow ion mobility distributions which may not be adequately sampled. For this study, optimal CCS determination (highest reproducibility with minimal RSD) was observed when utilizing seven electric fields ranging from 10.9–18.5 V/cm, with the field in the rear funnel set as the average field in this range to minimize fringing field effects (step 4 in Tables S3 and S4).…”

Section: Methodsmentioning

confidence: 99%

“…A fundamental assumption in Eqn (1) is that the separation is performed under so-called low-field conditions, or that ion heating is not appreciable between collisions. 11,13,14 When this assumption is satisfied, the resulting CCS value represents an orientationally-averaged cross sectional area of the molecule and drift gas interaction which is related to the microscopic size and shape of the molecule. Because the Mason-Schamp equation defines CCS as a function of several experimental parameters, these parameters must be well-characterized in order to obtain CCS values with high confidence, necessitating the use of uniform field DTIM instrumentation constructed under rigid mechanical tolerances and operated with high purity gases with well-defined pressure and temperature conditions.…”

Section: Introductionmentioning

confidence: 99%

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An Interlaboratory Evaluation of Drift Tube Ion Mobility–Mass Spectrometry Collision Cross Section Measurements

et al. 2017

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Collision cross section (CCS) measurements resulting from ion mobility – mass spectrometry (IM-MS) experiments provide a promising orthogonal dimension of structural information in MS based analytical separations. As with any molecular identifier, interlaboratory standardization must precede broad range integration into analytical workflows. In this study we present a reference drift tube ion mobility mass spectrometer (DTIM-MS) where improvements on the measurement accuracy of experimental parameters influencing IM separations provide standardized drift tube, nitrogen CCS values (DTCCSN2) for over 120 unique ion species with the lowest measurement uncertainty to date. The reproducibility of these DTCCSN2 values are evaluated across three additional laboratories on a commercially-available DTIM-MS instrument. The traditional stepped field CCS method performs with a relative standard deviation (RSD) of 0.29% for all ion species across the three additional laboratories. The calibrated single field CCS method, which is compatible with a wide range of chromatographic inlet systems, performs with an average, absolute bias of 0.54% to the standardized stepped field DTCCSN2 values on the reference system. The low RSD and biases observed in this interlaboratory study illustrate the potential of DTIM-MS for providing a molecular identifier for a broad range of discovery based analyses.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Interlaboratory Evaluation of Drift Tube Ion Mobility–Mass Spectrometry Collision Cross Section Measurements

et al. 2017

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“…4–7 Ion mobility (IM) mass spectrometry is a robust method that allows for the rapid separation and detection of a wide range of biologically important compounds. 8–10 The coupling of MALDI-MS with IM spectrometry (MALDI-IM MS) has allowed for a variety of samples to be analyzed and offers the potential for real-time separation, which operates within the several hundred microsecond time interval between the application of each focused laser desorption pulse to the sample. 10–13 In one study 14 , MALDI-IM MS was used to analyze and separate complex mixtures of phospholipids in positive mode.…”

Section: Introductionmentioning

confidence: 99%

“…8–10 The coupling of MALDI-MS with IM spectrometry (MALDI-IM MS) has allowed for a variety of samples to be analyzed and offers the potential for real-time separation, which operates within the several hundred microsecond time interval between the application of each focused laser desorption pulse to the sample. 10–13 In one study 14 , MALDI-IM MS was used to analyze and separate complex mixtures of phospholipids in positive mode. The change in phospholipids’ ion drift time was shown to be dictated by the following factors: 1) radyl chain length and its degree of unsaturation, 2) head group type, and 3) type of cationization (salt adducts) of individual species.…”

Section: Introductionmentioning

confidence: 99%

MALDI-ion mobility mass spectrometry of lipids in negative ion mode

et al. 2014

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Profiling and imaging MALDI mass spectrometry (MS) allows detection and localization of biomolecules in tissue, of which lipids are a major component. However, due to the in situ nature of this technique, complexity of tissue and need for a chemical matrix, the recorded signal is complex and can be difficult to assign. Ion mobility adds a dimension that provides coarse shape information, separating isobaric lipids, peptides, and oligonucleotides along distinct familial trend lines before mass analysis. Previous work using MALDI-ion mobility mass spectrometry to analyze and image lipids has been conducted mainly in positive ion mode, although several lipid classes ionize preferentially in negative ion mode. This work highlights recent data acquired in negative ion mode to detect glycerophosphoethanolamines (PEs), glycerophosphoserines (PSs), glycerophosphoglycerols (PGs), glycerolphosphoinositols (PIs), glycerophosphates (PAs), sulfatides (STs), and gangliosides from standard tissue extracts and directly from mouse brain tissue. In particular, this study focused on changes in ion mobility based upon lipid head groups, composition of radyl chain (# of carbons and double bonds), diacyl versus plasmalogen species, and hydroxylation of species. Finally, a MALDI-ion mobility imaging run was conducted in negative ion mode, resulting in the successful ion mapping of several lipid species.

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“…Although IM separations have existed for over a century and IM coupled with MS since the 1970s, the use of IM-MS for biological applications has really only emerged for complex systems over the past decade [48]. Largely this slow evolution of IM-MS is because until the past two years, biological IM-MS instruments were not commercially available and only existed in the labs of physicists and chemists willing to construct them.…”

Section: Introductionmentioning

confidence: 99%

Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform

Enders

Marasco

Kole

et al. 2010

IET Systems Biology

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The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels, and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental setup and control parameters and on-line desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signaling pathways.

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Ion Mobility–Mass Spectrometry

Cited by 14 publications

References 129 publications

An Interlaboratory Evaluation of Drift Tube Ion Mobility–Mass Spectrometry Collision Cross Section Measurements

An Interlaboratory Evaluation of Drift Tube Ion Mobility–Mass Spectrometry Collision Cross Section Measurements

MALDI-ion mobility mass spectrometry of lipids in negative ion mode

Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform

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