Tetragonal Heusler alloys are attracting more attention owing to their great scientific and technological interest in spintronics. However, the crystal structures of most Heusler alloys are cubic. One feasible method to obtain tetragonal Heusler alloy is to manipulate the out-of-plane distortion of cubic Heusler alloys. In this work, we report the ion irradiation-induced tetragonal distortion in Mn2CoAl Heusler films. The out-of-plane lattice constant continuously increases from 5.84 to 6.09 Å, while the in-plane lattice constant remains nearly unchanged. Magnetic measurements show that saturation magnetization at 50 K decreases from 258 emu/cc to 167 emu/cc under Ar ion irradiation. Transport measurements show that the longitudinal resistivity decreases with increasing irradiation doses, and the anomalous Hall resistivity changes from negative to positive. Moreover, it is found that hump-like anomalies can be observed in the Hall resistivity loops at the irradiation dose of 1×〖10〗^14 and 1×〖10〗^15 /cm2, which are attributed to the irradiation-induced inhomogeneous magnetism in the surface and interface parts. This work demonstrates the considerable tunability of the structure, magnetism and transport properties of Mn2CoAl Heusler alloys by Ar ion irradiation.