Ion-exchange phenomena and concomitant pH shifts on the equilibration of reversed-phase packings with ion-pairing reagents

Houwen, O.A.G.J. van der; Sorel, R; Hulshoff, A.; Teeuwsen, J.; Indemans, A. W. M.

doi:10.1016/s0021-9673(00)80607-x

Cited by 35 publications

(12 citation statements)

References 15 publications

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“…(only ionic strength effect). On SAS Hypersil, the "salting-out" effect seems to be less important, and ion exchange phenomena, enhanced by NaCl (25)(26)(27), can occur with surface silanols. The silanols have much greater affinity for CTA+ than for Na+ (28) and no affinity for anionic SDS.…”

Section: Resultsmentioning

confidence: 99%

Additive effects on surfactant adsorption and ionic solute retention in micellar liquid chromatography

Berthod¹,

Girard²,

Gonnet³

1986

Anal. Chem.

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Section: Resultsmentioning

confidence: 99%

Additive effects on surfactant adsorption and ionic solute retention in micellar liquid chromatography

Berthod¹,

Girard²,

Gonnet³

1986

Anal. Chem.

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“…Effect of Silanols on TBA+. Other workers have reported that the amount of TBA+ sorbed on reversed-phase bondedphase packings, such as Cig, increases with increasing mobile-phase pH (40,46,47,50). It has been suggested that this phenomenon is related to the fact that cationic samples sorb more strongly onto anionic silanolate sites by the process of cation exchange than they sorb onto silanol sites or onto ODS groups and that the number of silanolate sites increases with pH.…”

Section: Apparatus Andmentioning

confidence: 98%

“…Sorption of TBA+. A number of workers have studied the sorption of TBA+ on ODS packings, and some have reported that it is difficult to completely elute TBA+ with eluents that contain no electrolyte (40,41). This is presumably because anionic silanolate groups, arising from ionization of residual silanol group, constitute a cation exchanger.…”

Section: Apparatus Andmentioning

confidence: 99%

Electrical double-layer model for sorption of ions on octadecylsilyl bonded phases including the role of residual silanol groups

Liu¹,

Cantwell²

1991

Anal. Chem.

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with longer beam lines. Mass spectrometrists may be interested in exploring the technique's potential for high resolution.By pushing the technique with faster rise times of the BVM wave form, higher beam voltages, smaller BVM tubes, and energy selection of the continuous ion beam, vastly higher resolutions could be achieved. The technique shows great promise as a method for high-frequency population modulation and appears to be amenable to Fourier transform mass spectrometric techniques. ACKNOWLEDGMENTWe are also indebted to A. G. Marshall for discussions about this instrument and our neighbors who have lent us equipment, including R.

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“…Once the sites with easier access are filled (~40 min), the ion-pairing reagent begins to occupy the sites of the next accessible level in the column. The two-adsorption levels can be explained using the two main mechanisms that govern the adsorption behavior of the basic compounds on the C 18 stationary phase, hydrophobic interaction with the alkyl chains and ion-exchange with the acidic silanols (21,22,24). The adsorption initially occurs on easily accessible C 18 chains then eventually penetrates to the sub-layer of the silanol groups.…”

Section: Superimposed On the Ion Pairing Concentration Profiles Inmentioning

confidence: 99%

Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

Zhang

Raglione

Wang

et al. 2011

Journal of Chromatographic Science

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The regeneration of ion-pairing reagent distribution on liquid chromatography columns after gradient elution has been well recognized as the cause for long column equilibration time, a major drawback associated with gradient elution reverse phase ion-pair chromatography. To date, the majority of studies have focused on optimizing the separation conditions to shorten the equilibration time. There is limited understanding of the ion-pairing reagent distribution process between the mobile phase and stationary phase in the course of gradient elution, and subsequent column re-equilibration. The focus of this work is to gain a better understanding of this process. An ion-pair chromatographic system, equipped with a YMC ODS C(18) column and a mobile phase containing tetrabutylammonium (TBA) hydroxide as the ion-pairing reagent, was used in the study. The TBA distribution profile was established by measuring its concentration in the eluent fractions collected during the gradient cycle using different column equilibration times with an ion chromatographic method. Furthermore, the analyte retention time was evaluated as the function of the column equilibration time and TBA concentration in the mobile phase. The column equilibration and its impact on the method robustness will also be discussed.

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Ion-exchange phenomena and concomitant pH shifts on the equilibration of reversed-phase packings with ion-pairing reagents

Cited by 35 publications

References 15 publications

Additive effects on surfactant adsorption and ionic solute retention in micellar liquid chromatography

Additive effects on surfactant adsorption and ionic solute retention in micellar liquid chromatography

Electrical double-layer model for sorption of ions on octadecylsilyl bonded phases including the role of residual silanol groups

Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

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