Ion‐Exchange Adsorption of Proteins: Experiments and Molecular Dynamics Simulations

Liang, Juan; Fieg, Georg; Jakobtorweihen, Sven

doi:10.1002/cite.201400095

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…10 Some studies can be found in the literature based on ion exchange for protein adsorption and separation, such as the adsorption of bovine serum albumin, ovalbumin, and conalbumin on a strong anion exchanger by Field et al with measurement of the adsorption equilibrium isotherms for multicomponent mixtures, 11 while the adsorption of serum albumin and hemoglobin with the anion exchanger Q Sepharose FF was studied by Liang et al and the SMA model was implemented to describe the adsorption equilibrium. 12 Noh et al also studied the adsorption of three proteins, lysozyme, human serum albumin, and immunoglobulin G, on anion and cation exchangers. 13 There are also some studies regarding the evolution of protein concentration during adsorption experiments on ion exchangers.…”

Section: Introductionmentioning

confidence: 99%

Bovine Serum Albumin and Myoglobin Separation by Ion-Exchange SMB

Rios,

Ribeiro,

Rodrigues

et al. 2023

Ind. Eng. Chem. Res.

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The separation of the proteins bovine serum albumin (BSA) and myoglobin (Mb) was achieved experimentally by ion-exchange simulated moving bed (IE-SMB) using a salt step elution in an SMB bench-scale unit. The BSA adsorption equilibrium at different salt concentrations was determined by frontal analysis. A phenomenological mathematical model, i.e., the general rate model coupled with steric mass action equilibrium, was used and validated against fixed-bed dynamic adsorption experiments at different salt concentrations. The separation region was computed, and the operating conditions for an SMB experiment were selected within the obtained region. The chosen operating point was experimentally tested, and purity, recovery, and productivity of the BSA on the extract were 96%, 99%, and 4.93 × 10 −3 mol protein •kg ads −1 •day −1 and for the Mb on the raffinate were 99%, 96%, and 4.77 × 10 −3 mol protein •kg ads −1 •day −1 , respectively.

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Section: Introductionmentioning

confidence: 99%

Bovine Serum Albumin and Myoglobin Separation by Ion-Exchange SMB

Rios,

Ribeiro,

Rodrigues

et al. 2023

Ind. Eng. Chem. Res.

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Understanding adsorption behavior of α-chymotrypsin onto cation exchanger using all-atom molecular dynamics simulations

Tournois

Mathé

André

et al. 2020

Journal of Chromatography A

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Separation of aliphatic amino acids from aqueous media with an aminophosphonic ion exchanger

Bondareva,

Zagorulko,

Astapov

2023

Vestn. Voronež. gos. univ. inž. tehnol.

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The sorption of the aliphatic amino acids glycine and α-alanine by an aminophosphonic ion exchanger is studied and the dynamics of sorption is described using a kinetic equation. Experimental studies were carried out on a designed experimental setup with a fixed ion exchanger layer, in which the purified and regenerating solutions are passed through the sorbent layer in various directions. The heterogeneous ion exchange process includes the transport of sorbent ions in the liquid phase to the grain surface and the removal of desorbed ions from it, interphase transfer, diffusion of sorbed and desorbed ions inside the grain, since not all functional groups of the sorbent are localized on the surface, and a reversible ion exchange reaction. The kinetic equation of the Thomas model takes into account the multistage sorption and adequately describes the dependence of the degree of extraction of the component on the duration of contact of the solution with the ion exchanger layer. The equation of the Thomas model has been modernized taking into account the effect on the dynamics of the process of diffusion resistances in the channels of the layer and the grains of the ion exchanger in a column with a fixed loading. The upgraded model is applied to describe the dynamics of ion exchange of aliphatic amino acids on an aminophosphonic ion exchanger and the possibility of using a one-dimensional capillary flow model to estimate the diffusion resistance during fluid movement in the channels of the ion exchanger layer is shown. The agreement of the calculated and experimental output curves of sorption of aliphatic amino acids from aqueous solutions of various concentrations has been verified. It is shown that the upgraded model adequately describes the dependence of the degree of amino acid extraction on the duration of contact of the solution with the polyampholite layer at different feed rates of the purified solution.

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Ion‐Exchange Adsorption of Proteins: Experiments and Molecular Dynamics Simulations

Cited by 3 publications

References 40 publications

Bovine Serum Albumin and Myoglobin Separation by Ion-Exchange SMB

Bovine Serum Albumin and Myoglobin Separation by Ion-Exchange SMB

Understanding adsorption behavior of α-chymotrypsin onto cation exchanger using all-atom molecular dynamics simulations

Separation of aliphatic amino acids from aqueous media with an aminophosphonic ion exchanger

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