Ion Chromatography in Pharmaceutical Drug Analysis

Bhattacharyya, Lokesh

doi:10.1002/9781118147009.ch6

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…In order to overcome these problems, new techniques or already known methods, such as ion exchange chromatography (IEX), need to be developed and further adapted to the scalable EV isolation process. IEX has been successfully used in the clinical production of several monoclonal antibodies (Hardin et al, 2009) and is considered a key element in the robust and cost-effective production of future drugs (Bhattacharyya, 2012;Lu and Qi, 2021;Yuan et al, 2023). For the purification of EV, we chose to use tentacle ion exchangers, which are commonly used chromatography media with grafted polymers.…”

Section: Introductionmentioning

confidence: 99%

Novel insights into the isolation of extracellular vesicles by anion exchange chromatography

Koch,

Best,

Wüstenhagen

et al. 2024

Front. Bioeng. Biotechnol.

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Extracellular vesicles (EVs) are membrane structures enclosed by a lipid bilayer that are released into the extracellular space by all types of cells. EVs are involved in many physiological processes by transporting biologically active substances. Interest in EVs for diagnostic biomarker research and therapeutic drug delivery applications has increased in recent years. The realization of the full therapeutic potential of EVs is currently hampered by the lack of a suitable technology for the isolation and purification of EVs for downstream pharmaceutical applications. Anion Exchange Chromatography (AEX) is an established method in which specific charges on the AEX matrix can exploit charges on the surface of EVs and their interactions to provide a productive and scalable separation and purification method. The established AEX method using Eshmuno® Q, a strong tentacle anion exchange resin, was used to demonstrate the principal feasibility of AEX-based isolation and gain insight into isolated EV properties. Using several EV analysis techniques to provide a more detailed insight into EV populations during AEX isolation, we demonstrated that although the composition of CD9/63/81 remained constant for tetraspanin positive EVs, the size distribution and purity changed during elution. Higher salt concentrations eluted larger tetraspanin negative vesicles.

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Section: Introductionmentioning

confidence: 99%

Novel insights into the isolation of extracellular vesicles by anion exchange chromatography

Koch,

Best,

Wüstenhagen

et al. 2024

Front. Bioeng. Biotechnol.

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Trimethylsilylation of Polygalacturonic Acid

Würfel

Kayser

Heinze

2019

Macro Chemistry & Physics

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The trimethylsilylation of polygalacturonic acid is investigated in formamide as reaction medium at elevated temperature employing 1,1,1,3,3,3‐hexamethyldisilazan (HMDS) or trimethylsilyl chloride (TMSCl) in combination with imidazole. The products obtained are characterized by size exclusion chromatography, NMR‐, and IR spectroscopy. Regarding the degree of substitution (DS), HMDS is a more reactive silylation reagent compared to TMSCl/imidazole. Products with DS values of 0.6 are obtained with TMSCl while HMDS yields products with DS of up to 1.8. Nevertheless, both reagents lead to the formation of side products. In the case of HMDS, formation of amid moiety occurs, which renders the carboxylic acid at the polymer backbone inaccessible for further transformations. Cross‐linking of polymer chains occurs applying TMSCl, which influences the molecular masses of the products obtained.

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