Ion chromatographic separation of common mono- and divalent cations on an unmodified silica gel column by elution with oxalic acid containing crown ethers

Ohta, Kazutoku; Tanaka, Kazuhiko

doi:10.1039/a808382g

Cited by 12 publications

(7 citation statements)

References 10 publications

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“…NH 4 + , K + , Mg 2+ and Ca 2+ ) were achieved in 17 min by using 1 mmol dm 23 tyramine-0.2 mmol dm 23 oxalic acid-20 mmol dm 23 15-crown-5 at pH 4.75 as the eluent. As reported previously, 10 the Zorbax BP-SIL column was very effective in IC with conductimetric detection (IC-CD) for these cations. These results confirm that Zorbax BP-SIL silica gel is one of the best cation-exchange stationary phases not only for IC-CD but also for IC-IPD.…”

Section: Discussionsupporting

confidence: 69%

“…As reported previously, 10 the addition of 15-crown-5 to an acidic eluent was very effective for the improvement of peak resolution between mono-and divalent cations in IC-CD using the Zorbax BP-SIL column. Hence, the addition of 15-crown-5 to 1 mmol dm 23 tyramine-0.2 mmol dm 23 oxalic acid at pH 4.75 as the eluent was carried for the complete separation of these cations.…”

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...supporting

confidence: 66%

“…The order of the increase in the retention volumes was Mg 2+ < Ca 2+ . Since these orders were in good agreement with those in IC-CD using the Zorbax BP-SIL column and oxalic acid-15-crown-5 as the eluent, 10 it was also proved that 15-crown-5 was adsorbed on the silica stationary phase and acted as a selective cationexchanger under the IC-IPD conditions. Good separation of the mono-and divalent cations was achieved at a concentration of 15-crown-5 between 20 and 30 mmol dm 23 .…”

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...supporting

confidence: 65%

“…The suitable concentration in IC-IPD was considerably higher than that in IC-CD. 10 This suggested that the effect of 15-crown-5 was suppressed by protonated tyramine as the eluent ion, which was strongly adsorbed on the silica stationary phase.…”

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...mentioning

confidence: 99%

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Simultaneous separation of common mono- and divalent cations by ion chromatography with indirect photometric detection using an unmodified silica gel column and tyramine–oxalic acid–15-crown-5 as eluent

Ohta

2000

Analyst

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Section: Discussionsupporting

confidence: 69%

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...supporting

confidence: 66%

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...supporting

confidence: 65%

Section: Effect Of 15-crown-5 On Retention Behavior Of Mono-and Dival...mentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous separation of common mono- and divalent cations by ion chromatography with indirect photometric detection using an unmodified silica gel column and tyramine–oxalic acid–15-crown-5 as eluent

Ohta

2000

Analyst

Self Cite

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“…In contrast, the chaotropic bicarbonate and formate ions destabilize the protein structure, resulting in a larger hydrodynamic radius and, consequently, shorter elution times. Note that the difference between the potassium eluent and ammonium salts can also be attributed in part to the ability of potassium to more effectively shield the silica gel material. , …”

Section: Resultsmentioning

confidence: 99%

Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

et al. 2020

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Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether the applied eluent conditions actually prevent protein−stationary phase interactions, and/or protein denaturation, often is not assessed. In this study, the effects of volatile mobile phase additives on SEC retention and ESI of proteins were thoroughly investigated. Myoglobin was used as the main model protein, and eluents of varying ionic strength and pH were applied. The degree of interaction between protein and stationary phase was evaluated by calculating the SEC distribution coefficient. Protein-ion charge state distributions obtained during offline and online native ESI-MS were used to monitor alterations in protein structure. Interestingly, most of the supposedly mild eluent compositions induced nonideal SEC behavior and/or protein unfolding. SEC experiments revealed that the nature, ionic strength, and pH of the eluent affected protein retention. Protein−stationary phase interactions were effectively avoided using ammonium acetate at ionic strengths above 0.1 M. Direct-infusion ESI-MS showed that the tested volatile eluent salts seem to follow the Hofmeister series: no denaturation was induced using ammonium acetate (kosmotropic), whereas ammonium formate and bicarbonate (both chaotropic) caused structural changes. Using a mobile phase of 0.2 M ammonium acetate (pH 6.9), several proteins (i.e., myoglobin, carbonic anhydrase, and cytochrome c) could be analyzed by SEC-ESI-MS using different column chemistries without compromising their native state. Overall, with SEC-ESI-MS, the effect of nonspecific interactions between protein and stationary phase on the protein structure can be studied, even revealing gradual structural differences along a peak.

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Capillary Ion Chromatography Using 18-Crown-6 Ether as Eluent Additive for the Separation of Anions

2017

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Ion chromatographic separation of common mono- and divalent cations on an unmodified silica gel column by elution with oxalic acid containing crown ethers

Cited by 12 publications

References 10 publications

Simultaneous separation of common mono- and divalent cations by ion chromatography with indirect photometric detection using an unmodified silica gel column and tyramine–oxalic acid–15-crown-5 as eluent

Simultaneous separation of common mono- and divalent cations by ion chromatography with indirect photometric detection using an unmodified silica gel column and tyramine–oxalic acid–15-crown-5 as eluent

Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

Capillary Ion Chromatography Using 18-Crown-6 Ether as Eluent Additive for the Separation of Anions

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