Ion channels in postnatal neurogenesis

Swayne, Leigh Anne; Wicki-Stordeur, Leigh E.

doi:10.4161/chan.19721

Cited by 17 publications

(9 citation statements)

References 89 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, during osteoblast differentiation, K V 7.3 mRNA expression in these cells initially declined, then increased remarkably at day 14. Similar to our results, alterations of gene expressions during differentiation have been reported in studies of ion channels [ 26 , 71 , 72 ]. Variation of the pannexin 2 gene was observed in neurogenesis [ 71 ] and K V 3.1 transcript levels were shown to oscillate during adipogenesis [ 26 ].…”

Section: Discussionsupporting

confidence: 92%

“…Similar to our results, alterations of gene expressions during differentiation have been reported in studies of ion channels [ 26 , 71 , 72 ]. Variation of the pannexin 2 gene was observed in neurogenesis [ 71 ] and K V 3.1 transcript levels were shown to oscillate during adipogenesis [ 26 ]. Unlike the variation of K V 7.3 transcripts, expression of K V 7.3 proteins remained at the same levels, then was augmented after day 4.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

The Role of KV7.3 in Regulating Osteoblast Maturation and Mineralization

Yang

Song

Shen

et al. 2016

IJMS

View full text Add to dashboard Cite

KCNQ (KV7) channels are voltage-gated potassium (KV) channels, and the function of KV7 channels in muscles, neurons, and sensory cells is well established. We confirmed that overall blockade of KV channels with tetraethylammonium augmented the mineralization of bone-marrow-derived human mesenchymal stem cells during osteogenic differentiation, and we determined that KV7.3 was expressed in MG-63 and Saos-2 cells at the mRNA and protein levels. In addition, functional KV7 currents were detected in MG-63 cells. Inhibition of KV7.3 by linopirdine or XE991 increased the matrix mineralization during osteoblast differentiation. This was confirmed by alkaline phosphatase, osteocalcin, and osterix in MG-63 cells, whereas the expression of Runx2 showed no significant change. The extracellular glutamate secreted by osteoblasts was also measured to investigate its effect on MG-63 osteoblast differentiation. Blockade of KV7.3 promoted the release of glutamate via the phosphorylation of extracellular signal-regulated kinase 1/2-mediated upregulation of synapsin, and induced the deposition of type 1 collagen. However, activation of KV7.3 by flupirtine did not produce notable changes in matrix mineralization during osteoblast differentiation. These results suggest that KV7.3 could be a novel regulator in osteoblast differentiation.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

The Role of KV7.3 in Regulating Osteoblast Maturation and Mineralization

Yang

Song

Shen

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…First, the resting membrane potential of mature cortical neurons is hyperpolarized and averages between −60 and −80 mV (Bean 2007;McCormick et al 1985). This membrane potential is achieved by a highly regulated process of ion channel expression, particularly potassium channels, and hyperpolarization occurs gradually over time as these channels are expressed (Swayne and Wicki-Stordeur 2012). This observation is correlated with an age-dependent decrease in membrane resistance.…”

Section: Discussionmentioning

confidence: 99%

The effect of rho kinase inhibition on morphological and electrophysiological maturity in iPSC-derived neurons

et al. 2018

View full text Add to dashboard Cite

Induced pluripotent stem cell (iPSC)-derived neurons permit the study of neurogenesis and neurological disease in a human setting. However, the electrophysiological properties of iPSCderived neurons are consistent with those observed in immature cortical neurons, including a high membrane resistance, depolarized resting membrane potential, and immature firing properties, limiting their use in modelling neuronal activity in adult cells. Based on the proven association between inhibiting rho kinase (ROCK) and increased neurite complexity, we sought to determine if short-term ROCK inhibition during the first 1-2 weeks of differentiation would increase morphological complexity and electrophysiological maturity after several weeks of differentiation. While inhibiting ROCK resulted in increased neurite formation after 24 hours, this effect did not persist at 3 and 6 weeks of age. Additionally, there was no effect of ROCK inhibition on electrophysiological properties at 2-3, 6, or 12 weeks of age, despite an increase in evoked and spontaneous firing and a more hyperpolarized resting membrane potential over time. These results indicates that while there is a clear effect of time on electrophysiological maturity, ROCK inhibition did not accelerate maturity.

show abstract

“…Post-stroke NVU remodeling represents a growing field in neuropathophysiology (Maki et al, 2013;Knowland et al, 2014;Liu et al, 2014;Prakash and Carmichael, 2015;Munji et al, 2019). While the mechanisms underlying ischemia-induced neuronal plasticity are an ongoing focus in stroke research (Mostany et al, 2010;Swayne and Wicki-Stordeur, 2012;Silasi and Murphy, 2014;Felling and Song, 2015), our understanding of acute and chronic vascular responses to stroke is only in its infancy. NVU remodeling is rapidly activated after stroke and occurs at the molecular and cellular levels.…”

Section: General Conceptsmentioning

confidence: 99%