Ion channels as potential redox sensors in lysosomes

Yu, Jie

doi:10.1080/19336950.2019.1684428

Cited by 6 publications

(4 citation statements)

References 65 publications

(76 reference statements)

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“…To circumvent the anti-proliferative effect of a high NAC or ascorbic acid concentration on cancer cells, we verified the optimal NAC or ascorbic acid concentration for our experiment by performing cell counting kit-8 (CCK-8) assay ( 31 , 32 ). Several ion channels including transient receptor potential mucolipin-1 (TRPML1), TRPML2, and TRP channels have actually been identified as redox sensors on plasma membrane or intracellular organelles ( 33 , 34 ). Specifically, TRPML2 can exhibit ROS-sensing capability in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration

et al. 2021

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Voltage-gated potassium (Kv) channels are involved in many important cellular functions and play pivotal roles in cancer progression. The expression level of Kv2.1 was observed to be higher in the highly metastatic prostate cancer cells (PC-3), specifically in their membrane, than in immortalized prostate cells (WPMY-1 cells) and comparatively less metastatic prostate cancer cells (LNCaP and DU145 cells). However, Kv2.1 expression was significantly decreased when the cells were treated with anti-oxidants, such as N-acetylcysteine or ascorbic acid, implying that the highly expressed Kv2.1 could detect reactive oxygen species (ROS) in malignant prostate cancer cells. In addition, the blockade of Kv2.1 with stromatoxin-1 or siRNA targeting Kv2.1 significantly inhibited the migration of malignant prostate cancer cells. Our results suggested that Kv2.1 plays an important role as a ROS sensor and that it is a promising therapeutic molecular target in metastasis of prostate cancer.

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Section: Discussionmentioning

confidence: 99%

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration

et al. 2021

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“…Oxidised mTOR can be rescued by Thioredoxin 1 [ 264 ]. Related to this, lysosomes can also sense redox signalling specifically via redox-sensing lysosomal ion channels [ 265 ].…”

Section: Autophagy and Redoxtasis Crosstalkmentioning

confidence: 99%

Autophagy and Redox Homeostasis in Parkinson’s: A Crucial Balancing Act

Jiménez-Moreno

Lane

2020

Oxidative Medicine and Cellular Longevity

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Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated primarily from endogenous biochemical reactions in mitochondria, endoplasmic reticulum (ER), and peroxisomes. Typically, ROS/RNS correlate with oxidative damage and cell death; however, free radicals are also crucial for normal cellular functions, including supporting neuronal homeostasis. ROS/RNS levels influence and are influenced by antioxidant systems, including the catabolic autophagy pathways. Autophagy is an intracellular lysosomal degradation process by which invasive, damaged, or redundant cytoplasmic components, including microorganisms and defunct organelles, are removed to maintain cellular homeostasis. This process is particularly important in neurons that are required to cope with prolonged and sustained operational stress. Consequently, autophagy is a primary line of protection against neurodegenerative diseases. Parkinson’s is caused by the loss of midbrain dopaminergic neurons (mDANs), resulting in progressive disruption of the nigrostriatal pathway, leading to motor, behavioural, and cognitive impairments. Mitochondrial dysfunction, with associated increases in oxidative stress, and declining proteostasis control, are key contributors during mDAN demise in Parkinson’s. In this review, we analyse the crosstalk between autophagy and redoxtasis, including the molecular mechanisms involved and the detrimental effect of an imbalance in the pathogenesis of Parkinson’s.

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“…The continuity of the endoplasmic reticulum with the nuclear membrane is a clear example of such a problem. Second, the unique microenvironment (both functionally and structurally) of intracellular compartments (including mitochondria) affects in vitro studies of various ion channels [5][6][7]. These issues will be discussed in greater detail later in this paper.…”

Section: Introductionmentioning

confidence: 99%

“…cluding mitochondria) affects in vitro studies of various ion channels [5][6][7]. These issues will be discussed in greater detail later in this paper.…”

Section: Introductionmentioning

confidence: 99%

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

Walewska

Krajewska

Stefanowska

et al. 2022

IJMS

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In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we focus on the critical evaluation of techniques used in the studies of mitochondrial potassium channels, describing their advantages and limitations. Functional analysis of mitochondrial potassium channels in comparison to that of plasmalemmal channels presents additional experimental challenges. The reliability of functional studies of mitochondrial potassium channels is often affected by the need to isolate mitochondria and by functional properties of mitochondria such as respiration, metabolic activity, swelling capacity, or high electrical potential. Three types of techniques are critically evaluated: electrophysiological techniques, potassium flux measurements, and biochemical techniques related to potassium flux measurements. Finally, new possible approaches to the study of the function of mitochondrial potassium channels are presented. We hope that this review will assist researchers in selecting reliable methods for studying, e.g., the effects of drugs on mitochondrial potassium channel function. Additionally, this review should aid in the critical evaluation of the results reported in various articles on mitochondrial potassium channels.

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Ion channels as potential redox sensors in lysosomes

Cited by 6 publications

References 65 publications

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration

Autophagy and Redox Homeostasis in Parkinson’s: A Crucial Balancing Act

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

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