Ion channels as key partners of cytoskeleton in cancer disease

Rodat-Despoix, Lise; Chamlali, Mohamed; Ouadid-Ahidouch, Halima

doi:10.1016/j.bbcan.2021.188627

Cited by 5 publications

(2 citation statements)

References 128 publications

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“…Most of the peptides, which intensity was influenced by auranofin presence, represented cytoskeleton proteins. The cell cytoskeleton forms a complex system responsible for signal transduction, regulation of cellular transport, mitochondrial activity, morphology, and rearrangement [ 56 , 57 ]. It cannot be ignored, especially since it can explain why cells accumulate metal ions, even in the presence of a metallodrug.…”

Section: Resultsmentioning

confidence: 99%

Speciation Analysis Highlights the Interactions of Auranofin with the Cytoskeleton Proteins of Lung Cancer Cells

et al. 2022

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Two types of lung cells (epithelial cancer lung cells, A-549 and lung fibroblasts MRC-5) were exposed to the clinically established gold drug auranofin at concentrations close to the half-maximal inhibitory drug concentrations (IC50). Collected cells were subjected to speciation analysis using inductively coupled plasma mass spectrometry (ICP-MS). Auranofin showed better affinity toward proteins than DNA, RNA, and hydrophilic small molecular weight compounds. It can bind to proteins that vary in size (~20 kDa, ~75 kDa, and ≥200 kDa) and pI. However, the possibility of dimerization and protein–protein complex formation should also be taken into account. µRPLC/CZE-ESI-MS/MS studies on trypsinized proteins allowed the indication of 76 peptides for which signal intensity was influenced by auranofin presence in cells. Based on it, identity was proposed for 20 proteins. Except for thioredoxin reductase (TrxR), which is directly targeted by gold complex, the proteins were found to be transformed. Five indicated proteins: myosin, plectin, talin, two annexins, and kinase M3K5, are responsible for cell–cell, cell–protein interactions, and cell motility. A wound healing test confirmed their regulation by auranofin as cell migration decreased by 40% while the cell cycle was not interrupted.

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Section: Resultsmentioning

confidence: 99%

Speciation Analysis Highlights the Interactions of Auranofin with the Cytoskeleton Proteins of Lung Cancer Cells

et al. 2022

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“…Membrane ion transport systems are the gatekeepers for cells and organelles, thereby controlling the transportation of various exogenous/endogenous substances via influx/efflux mechanisms. Recent studies have uncovered the remodeling of ion homeostasis during cancer progression and explored the novel functions of membrane ion transport systems in the regulation of tumor malignancy (see reviews [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ]). However, little information is available on the roles of membrane ion transport systems in the neoplastic transformation and progression of cervical epithelial cells.…”

Section: Introduction: An Overview Of the Epidemiology And Pathogenesis Of Cervical Cancermentioning

confidence: 99%

The Important Role of Ion Transport System in Cervical Cancer

Chen

Shen

2021

IJMS

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Cervical cancer is a significant gynecological cancer and causes cancer-related deaths worldwide. Human papillomavirus (HPV) is implicated in the etiology of cervical malignancy. However, much evidence indicates that HPV infection is a necessary but not sufficient cause in cervical carcinogenesis. Therefore, the cellular pathophysiology of cervical cancer is worthy of study. This review summarizes the recent findings concerning the ion transport processes involved in cell volume regulation and intracellular Ca2+ homeostasis of epithelial cells and how these transport systems are themselves regulated by the tumor microenvironment. For cell volume regulation, we focused on the volume-sensitive Cl− channels and K+-Cl− cotransporter (KCC) family, important regulators for ionic and osmotic homeostasis of epithelial cells. Regarding intracellular Ca2+ homeostasis, the Ca2+ store sensor STIM molecules and plasma membrane Ca2+ channel Orai proteins, the predominant Ca2+ entry mechanism in epithelial cells, are discussed. Furthermore, we evaluate the potential of these membrane ion transport systems as diagnostic biomarkers and pharmacological interventions and highlight the challenges.

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