In this letter, we focus on the relationship between modification bioparticle density (N) and biological regulation ability in extended gate HEMT (EG-HEMT) biosensors. By analyzing, we demonstrate that under the same gate electrodes size (Sg) and concentration, N is exponentially related to the area of sensing regions (y) and has fixed exponential magnification -0.65 ±0.05. This formula had been demonstrated to have perfect universality when test protein and small molecule. Finally, we apply N to the structural design of EG-HEMT biosensors and projected that 10y structure has both high biological regulation ability of biosensors and efficient utility of chips.