Ion and Water Fluxes in the Ileum of Rats

Curran, Peter F.; Solomon, A. K.

doi:10.1085/jgp.41.1.143

Cited by 276 publications

(113 citation statements)

References 27 publications

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“…During the last few years there has been a resurgence of interest in these potentials in relation to active transport mechanisms and intestinal metabolism. Curran & Solomon (1957) studied the potential across the rat ileum in vivo and when the sodium concentration in the intestinal lumen was 146-163 m-equiv/l. the potential was less INTESTINAL POTENTIALS than 1 mV.…”

Section: Discussionmentioning

confidence: 99%

Electrical potentials associated with intestinal sugar transfer

Barry¹,

Dikstein²,

Matthews³

et al. 1964

The Journal of Physiology

148

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The rat small intestine has the capacity to transfer a number of substances, including hexoses, inorganic salts and fluid. Riklis & Quastel (1958) showed that movement of hexose was dependent on the presence of sodium, and Barry, showed that a part of the fluid transfer was dependent on the presence of glucose, and part was independent of it. It is generally assumed that fluid movement in living tissues is related to movement of inorganic salts, and there must therefore be a complex inter-relation between transfer of sodium, glucose and fluid. Since electrical changes in other tissues are related to ion pumps, an investigation was undertaken into the relation between the electrical potential across the wall of the intestine and the transfer of fluid, various ions, hexoses and their derivatives. The present paper deals with transfer of fluid and sugars in relation to electrical changes, and preliminary communications of the results have been given by Barry, Dikstein, Matthews & Smyth (1961), and Barry, Matthews, Smyth & Wright (1962). METHODSWhite male rats of the Sheffield strain were used. Before experiments the rats were maintained on an unrestricted diet of rat cubes No. 86 (Oxoid, London). The work included three different kinds of experiments: (1) the effect of hexoses on the electrical potential difference across the gut wall, and this was studied both in vitro and in vivo; (2) determination of the rate of fluid transfer maintained by different sugars or their derivatives; and (3) the extent of metabolism of these sugars by the intestine: these last two were studied by in vitro methods. In the in vitro experiments the intestine was suspended in bicarbonate saline (Krebs & Henseleit, 1932), which was in equilibrium with a gas mixture of 5 % CO2 and 95 % 02-When anoxic conditions were required a gas mixture of 5 % CO2 and 95 % N2 was used.Measurement of electrical potentials across intestine Electrical recording. The potentials were led off from the solutions on each side of the gut wall to calomel half-cells by means of polythene tubes filled with M-KCI-3 %-agar, and these tubes are referred to subsequently as salt bridges. The pairs of calomel half-cells were selected to balance each other to within 1 mV. The half-cells were connected with a Hewlett-Packard vacuum tube voltmeter, with a minimum input impedance of 10 MO.

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Section: Discussionmentioning

confidence: 99%

Electrical potentials associated with intestinal sugar transfer

Barry¹,

Dikstein²,

Matthews³

et al. 1964

The Journal of Physiology

148

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“…The perfusion of the gut was carried out by a modification of the procedure described by Curran & Solomon (1957). Male rats of the Wistar strain weighing 250±50 g were fasted overnight and then anaesthetized with intraperitoneal urethane 1 g/kg.…”

Section: Methodsmentioning

confidence: 99%

Absorption of Folic Acid From the Small Intestine of the Rat

Burgen

Goldberg

1962

British Journal of Pharmacology and Chemotherapy

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The absorption of folic acid from segments of the small intestine of the rat has been measured in situ with tritium-labelled folic acid. The fraction absorbed was independent of concentration below 106 M but was depressed to half at 4x10-5 M.Direct measurements of fluxes showed that the mucosal -e serosal flux was about 14 times the serosal -> mucosal flux, and therefore that uptake of folic acid is an active process. In the ileum but not in the duodeno-jejunum, absorption was depressed by the presence of electrolyte. There was little difference in absorptive capacity between jejunum and ileum nor was there any significant change in animals suffering from a dietary deficiency of folic acid.

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“…These include frog stomach (Hogben, 1955), frog skin glands (Koefoed-Johnson, Ussing & Zerahn, 354 ION FLUXES ACROSS LUNG EPITHELIUM 1952), cray-fish gills (Shaw, 1960), gold-fish gills (Garcia Romeu & Maetz, 1964), rat ileum (Curran & Solomon, 1957), locust rectum (Phillips, 1964) and sheep rumen (Ferreira, Harrison, Keynes & Zurich, 1972).…”

Section: R E Olver and L B Strang Secretion And Absorption Of Lunmentioning

confidence: 99%

Ion fluxes across the pulmonary epithelium and the secretion of lung liquid in the foetal lamb

Olver¹,

Strang²

1974

The Journal of Physiology

312

168

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2. The permeability sequence of the pulmonary epithelium for alkali metals was, Na+ > K+ > Rb+ > Li+ > Cs+ and that for halides I-Br-> C1-. Permeabilities to alkaline earths were lower than for the other ions, no definite sequence being established.3. There was an electrical potential difference of -1 to -10 mV (mean -4.3 mV) between lung liquid and plasma (lung liquid negative). Plasma/lung liquid chemical activity ratios were less than unity for the halides (Cl-, Br-, I-), and for K+ and Rb+, whereas the ratio of oneway fluxes (plasma --lung liquid)/(lung liquid -+ plasma) was in each case greater than unity. From the difference between the measured flux ratios and those predicted from the forces determining passive flux, it was concluded that the halides, K+ and Rb+ were actively transported from plasma to lung liquid, Cl-being quantitatively the most important. Na+ and Ca2+ appeared to move passively down a gradient of electrochemical potential.R. E. OLVER AND L. B. STRANG 4. When alveolar liquid [HCO ] was artificially raised, a net flux of HCO-from lung liquid against a gradient of electrochemical activity was observed, suggesting active transport of that ion out of lung liquid.5. The addition of KCN to lung liquid stopped the secretion of liquid and absorption took place.

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Ion and Water Fluxes in the Ileum of Rats

Cited by 276 publications

References 27 publications

Electrical potentials associated with intestinal sugar transfer

Electrical potentials associated with intestinal sugar transfer

Absorption of Folic Acid From the Small Intestine of the Rat

Ion fluxes across the pulmonary epithelium and the secretion of lung liquid in the foetal lamb

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