Iodoacetamide treatment of bovine leukemia virus glycoprotein gp51 enhances the Western blotting reactivity of anti&amp;#x2010;peptide antibodies

Callebaut, Isabelle; Burny, Arsène; Portetelle, Daniel

doi:10.1016/0014-5793(91)80854-v

Cited by 4 publications

(1 citation statement)

References 5 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As outlined in Table 1 [ 18 , [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] , [89] , [90] , [91] , [92] , [93] , [94] , [95] , [96] , [97] , [98] , [99] , [100] , [101] , [102] , [103] , [104] , [105] , [106] , [107] , [108] , [109] , [110] , [111] , [112] , [113] , [114] , [115] , [116] , [117] , [118] , [119] , [120] , [121] , [122] , [123] , [124] , [125] , [126] ,…”

Section: Metabolic Interventions In Covid-19unclassified

Can a metabolism-targeted therapeutic intervention successfully subjugate SARS-COV-2? A scientific rational

Mansouri

Rastegari-Pouyani

Ghanbri-Movahed

et al. 2020

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

show abstract

Section: Metabolic Interventions In Covid-19unclassified

Can a metabolism-targeted therapeutic intervention successfully subjugate SARS-COV-2? A scientific rational

Mansouri

Rastegari-Pouyani

Ghanbri-Movahed

et al. 2020

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

show abstract

Mapping of sequential epitopes recognized by monoclonal antibodies on the bovine leukaemia virus external glycoproteins expressed in Escherichia coli by means of antipeptide antibodies

Ban

Czene

Altaner

et al. 1992

Journal of General Virology

View full text Add to dashboard Cite

A 2 gtl 1 cDNA library prepared from bovine leukaemia virus (BLV)-producing ovine cells was screened with a cocktail of anti-BLV gp51 monoclonal antibodies (MAbs). Four recombinant phages with inserts of about 2-5 kbp were isolated. One, 2 BLV-gp51-1, was sequenced and shown to encode the C-terminal part of gpS1 and all of gp30. This insert was subcloned into pEV-vrfl and expressed in Escherichia coli N-4830-1 cells. The BLV product and a series of antipeptide antibodies were used to localize the sequential epitopes defined on BLV envelope glycoprotein gp51 by their reactivity with MAbs. Epitope B was localized to amino acids 180 to 205, B' to residues 195 to 205, D and D' to residues 218 to 237, and A to amino acids 249 to 260. All the mapped sequential epitopes were localized in the C-terminal half of BLV gp51. The results of epitope mapping with bacterially produced gp51 confirm the map obtained using native viral glycoprotein.

show abstract

Mapping of B-neutralizing and T-helper cell epitopes on the bovine leukemia virus external glycoprotein gp51

Callebaut

Voneche

Mager

et al. 1993

J Virol

View full text Add to dashboard Cite

A battery of 19 synthetic peptides was used to characterize efficient neutralizing and helper T-celi epitopes on the bovine leukemia virus (BLV) external envelope glycoprotein gp5l. Four of the antipeptide antisera raised in rabbits inhibited the formation of BLV-induced syncytia; these antisera are directed against peptides 64-73, 98-117, and 177-192. Only antisera directed against the 177-192 region also neutralized vesicular stomatis virus-BLV pseudotypes. This study clearly demonstrates that neutralizing properties can be observed with antibodies raised to regions undescribed so far and induded in both the amino-terminal and central parts of the antigen. In addition, some helper T-cell determinants were defined from gpSl-immunized mice and from BLV-infected cattle. Although none of the peptides tested behaved as a universal helper T-celi epitope, peptide 98-117 stimulated T-cell proliferation from BALB/c mice and from three infected cows, while peptide 169-188 strongly stimulated T-cell proliferation from one infected cow. Further experiments performed with three peptides overlapping the 169-188 region (177-192, 179-192, 181-192) demonstrated the particular relevance of residue(s) P-177 and/or D-178 in the helper T-celi epitope. These data should assist in the design of an efficient subunit vaccine against BLV infection that contains peptides possessing both B-neutralizing and helper T-cell determinants.

show abstract

Iodoacetamide treatment of bovine leukemia virus glycoprotein gp51 enhances the Western blotting reactivity of anti‐peptide antibodies

Cited by 4 publications

References 5 publications

Can a metabolism-targeted therapeutic intervention successfully subjugate SARS-COV-2? A scientific rational

Can a metabolism-targeted therapeutic intervention successfully subjugate SARS-COV-2? A scientific rational

Mapping of sequential epitopes recognized by monoclonal antibodies on the bovine leukaemia virus external glycoproteins expressed in Escherichia coli by means of antipeptide antibodies

Mapping of B-neutralizing and T-helper cell epitopes on the bovine leukemia virus external glycoprotein gp51

Contact Info

Product

Resources

About