Iodide Modified Silver Electrode and Its Application to the Electroanalysis of Hemoglobin

Fan, Chunhai; Li, Genxi; Yi, Zichuan; Zhu, Jianquin; Zhu, Dunru

doi:10.1002/(sici)1521-4109(200002)12:3<205::aid-elan205>3.0.co;2-y

Cited by 26 publications

(14 citation statements)

References 24 publications

(17 reference statements)

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“…Some reports have used electrochemical methods for the investigation of folding or unfolding of proteins as well as the effects of ligand binding and inorganic solvents on the structure of some haemoproteins such as cytochrome c, myoglobin and haemoglobin [17][18][19][20][21][22][23][24]. Moosavi-Movahedi et al reported that electrochemical methods can be used to determine the structural changes during the transition from unfolded cytochrome c to the folded structure [14].…”

Section: Introductionsupporting

confidence: 90%

Use of silver nanoparticles as an electron transfer facilitator in electrochemical ligand-binding of haemoglobin

et al. 2007

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Silver nanoparticles have an activity for high intensity electron transfer. They can facilitate the electron transfer from the redox centre of a protein, as a high volume molecule, to the electrode surface. In this study, silver nanoparticles were deposited on the surface of a graphite carbon electrode in the 1 V potential region. Deposition of silver nanoparticles, with a diameter between 70 and 150 nm, was observed on the graphite electrode by transmission electron microscopy (TEM). The results demonstrated that the fine redox waves of haemoglobin could be achieved after modification of the graphite electrode by silver nanoparticles. The cathodic and anodic peaks of haemoglobin were at -135 and +375 mV vs. Ag/AgCl, respectively. The effect of guanosine 3¢,5¢-triphosphate (GTP), guanosine diphosphate (GDP) and guanosine monophosphate (GMP) on the structure of haemoglobin was investigated. It was observed that GTP shifts the cathodic and anodic peaks positively, indicating the transfer of the haem group to the surface of protein as a reflex of easier oxidation and reduction, while GDP and GMP do not show this behaviour. GTP binds with haemoglobin, while GDP and GMP do not.

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Section: Introductionsupporting

confidence: 90%

Use of silver nanoparticles as an electron transfer facilitator in electrochemical ligand-binding of haemoglobin

et al. 2007

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“…Zhang et al (2004) also studied the structural changes of haemoglobin in the presence of different concentrations of glycerol by using haemoglobin immobilized on the surface of a pyrolytic graphite electrode. Fan et al (2000) reported that an iodide-modifi ed silver electrode could show cathodic and anodic peaks for haemoglobin in solution. Cotton's research on Surface Enhanced Resonant Raman Scattering (SERRS) showed that a complex would be formed on the surface of silver after it was treated with iodide (Sibbald et al 1996;Sibbald et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical investigation of the effect of some organic phosphates on haemoglobin

et al. 2007

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The effects of DPG,IHP,GTP,GDP and GMP on the structure and stability of haemoglobin were electrochemically investigated with an iodide-modified silver electrode in 0.01 M KNO 3 at pH 7.0.Anodic and cathodic peaks of haemoglobin were observed at 250 mV and 12 mV with a formal potential value of 133 mV vs.Ag/AgCl.The effects of different concentrations of DPG,IHP,GTP,GDP and GMP on the anaerobic redox reaction were determined. The results showed that DPG and IHP can lead to a positive shift in the reduction peak of haemoglobin,indicating that the oxidation peak shift of haemoglobin was small as a result of stabilization of the reduced state and destabilization of the R-like state of haemoglobin.GTP elicited a more positive shift in the cathodic and anodic peaks of haemoglobin at a higher concentration,signifying that it has a low-affinity binding site on haemoglobin.The positive shift of the cathodic and anodic peaks revealed a slight variation in the structure and indicated the unfolding of haemoglobin in the presence of high concentrations of GTP.Our study also showed that GDP and GMP did not cause significant shift the cathodic and anodic peaks of haemoglobin even at high concentrations,refuting the existence of specific GDP-and GMP-binding sites on the protein.Moreover,the iodide-modified silver electrode method proved to be easy and useful in investigating the effects of ligands or other effectors on haemoglobin in solution.

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“…6). The detection limit is lower than that for the electroanalysis of hemoglobin with a silver electrode modified by iodide [13] or a lipoic acid monolayer [18], but higher relative to gold electrodes modified by cysteamine monolayers [21].…”

Section: Resultsmentioning

confidence: 99%

“…As an important protein participating in oxygen transfer within the vertebrate, hemoglobin has been extensively investigated by electrochemical techniques due to its redox heme group [9,10,11,12,13,14,15,16]. In order to promote the electrochemical response of hemoglobin, the silver electrodes modified by cysteine [17] and lipoic acid [18] monolayers have been utilized.…”

Section: Introductionmentioning

confidence: 99%

Application of a gold electrode, modified by a self-assembled monolayer of 2-mercaptodecylhydroquinone, to the electroanalysis of hemoglobin

2003

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A gold electrode modified by a self-assembled monolayer of 2-mercaptodecylhydroquinone (H(2)Q(CH(2))(10)SH) was applied to investigate the electrochemical response of hemoglobin in aerated buffer solutions. Compared with a bare gold electrode, the monolayer of H(2)Q(CH(2))(10)SH could suppress the reduction wave of dissolved oxygen in the buffer while effectively promoting the rate of electron transfer between hemoglobin and the electrode. Thus, a convenient way for electroanalysis of hemoglobin in air was achieved at the H(2)Q(CH(2))(10)SH/Au electrode. A linear relationship existed between peak current and concentration of hemoglobin in the range 1 x 10(-7)-1 x 10(-6) mol L(-1).

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Iodide Modified Silver Electrode and Its Application to the Electroanalysis of Hemoglobin

Cited by 26 publications

References 24 publications

Use of silver nanoparticles as an electron transfer facilitator in electrochemical ligand-binding of haemoglobin

Use of silver nanoparticles as an electron transfer facilitator in electrochemical ligand-binding of haemoglobin

Electrochemical investigation of the effect of some organic phosphates on haemoglobin

Application of a gold electrode, modified by a self-assembled monolayer of 2-mercaptodecylhydroquinone, to the electroanalysis of hemoglobin

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