Involvement of tumor necrosis factor (TNF)-α in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin edema in mice

Murakawa, Masao; Yamaoka, Kiyoshi; Tanaka, Yoshitaka; Fukuda, Yoshiaki

doi:10.1016/j.bcp.2006.01.005

Cited by 150 publications

(89 citation statements)

References 21 publications

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“…17,18) As shown in Figs. 3A, 3B, and 3C, TPA challenge resulted in dramatic increases in TNF-α, IL-6, and IL-1β protein levels in ear biopsy homogenates.…”

Section: Effect Of Topical Application Of Ca On the Protein And Mrna mentioning

confidence: 71%

Caffeic Acid Reduces Cutaneous Tumor Necrosis Factor Alpha (TNF-α), IL-6 and IL-1β Levels and Ameliorates Skin Edema in Acute and Chronic Model of Cutaneous Inflammation in Mice

Zhang

Zhou

Wang

et al. 2014

Biological & Pharmaceutical Bulletin

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Caffeic acid (3,4-dihydroxycinnamic acid, CA) has been reported to have anti-inflammatory activity in animal models. However, the mechanisms underlying the anti-inflammatory effects of CA in skin inflammation are only partially understood. The present study was designed to investigate the effects and mechanisms of CA on acute and chronic skin inflammation in mice and the effect of CA in keratinocytes in vitro. The results showed that topical treatment with CA inhibited 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced skin edema in a dose-dependent manner, leading to substantial reductions in skin thickness and tissue weight, neutrophil-mediated myeloperoxidase activity, and various histopathological indicators. The CA treatment also significantly reduced the mRNA and protein levels of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and IL-1β at the application site, and the TNF-α production, the TNF-α-induced IL-6 and IL-1β production, and TNF-α-induced nuclear factor-kappa B (NF-κB) activation in human keratinocytes in vitro. Furthermore, CA was effective at reducing inflammatory damage induced by chronic TPA exposure. These results demonstrate that CA has anti-inflammatory activities in both acute and chronic contact dermatitis models via blockade of the mRNA and protein synthesis of these cytokines and neutrophil-mediated myeloperoxidase activity, and can target inflammatory mediators specifically in the keratinocytes. Taken together, the present results suggest that CA might be a therapeutic agent against inflammatory skin diseases.Key words 3,4-dihydroxycinnamic acid; pro-inflammatory cytokine; keratinocyte; 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced ear edema modelThe skin is an immune organ consisting of epidermal cells, i.e., keratinocytes and Langerhans cells.

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“…17,18) As shown in Figs. 3A, 3B, and 3C, TPA challenge resulted in dramatic increases in TNF-α, IL-6, and IL-1β protein levels in ear biopsy homogenates.…”

Section: Effect Of Topical Application Of Ca On the Protein And Mrna mentioning

confidence: 71%

Caffeic Acid Reduces Cutaneous Tumor Necrosis Factor Alpha (TNF-α), IL-6 and IL-1β Levels and Ameliorates Skin Edema in Acute and Chronic Model of Cutaneous Inflammation in Mice

Zhang

Zhou

Wang

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Protein kinase C which is activated by TPA (68) and responsible for transducing the inflammatory response cascade in the TPA-induced mouse ear oedema model has been ruled out as a target, with negative results obtained from screening one of the active compounds against all isoforms of this kinase. Time-response data obtained in earlier in vivo work suggests that the compounds are acting at an early stage of the inflammatory response, as indicated by a shallower slope of the data line ( Figure 4) (69). In this particular animal model steroid-like compounds often give similar patterns of data, in contrast to NSAID compounds which often show a delayed response and hence a steeper slope in the early stages (ie.…”

Section: Kuuku I'yu Medicinal Plant Projectmentioning

confidence: 65%

Learning from Both Sides: Experiences and Opportunities in the Investigation of Australian Aboriginal Medicinal Plants

Simpson¹,

Claudie

Smith³

et al. 2013

J Pharm Pharm Sci

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-With one of the oldest surviving cultures in the world, Australian Aboriginal people have developed immense knowledge about the diverse Australian flora. Western scientific investigation of some Australian Aboriginal medicinal plants has demonstrated interesting pharmacological activities and chemistry, however the majority of these species have not yet been extensively examined. We argue that research that is locally initiated and driven by Indigenous traditional owners in collaboration with Western scientists has significant potential to develop new plant-based products. Locally driven medicinal plants research in which traditional owners work as researchers in collaboration with University-based colleagues in the investigation of medicines rather than "stakeholders" or "informants" is one model that may be used in characterising plants with the potential to be developed into sustainable plant-based medicinal products with commercial value. Our team has taken this approach in research located both on traditional homelands and in the laboratory.

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“…***p < 0.001, *p < 0.05; NS: not significant versus control. synthesis of inflammatory eicosanoids by cyclooxygenase and 5-lipoxygenase enzymes (Wang et al 2001;Murakawa et al 2006). The effect of methanol extract on croton oil-induced ear oedema is probably attributed to lipophilic methanol-soluble substances that are able to penetrate through the skin barrier (Okoli et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical screening and anti-inflammatory properties of AlgerianHertia cheirifoliamethanol extract

Bouriche

Kada

Assaf

et al. 2016

Pharmaceutical Biology

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Context: Hertia cheirifolia L. (Asteraceae) is traditionally used in Northern Africa to treat various inflammatory infections. However, few studies on this plant have been reported. Objective: The anti-inflammatory activity of methanol extract of H. cheirifolia leaves was investigated using different experimental models. Materials and methods: Phytochemical analysis was performed to determine phenolic compounds. Acute toxicity of the extract (2000 mg/kg) was examined in Swiss albino mice for 14 days, before croton oilinduced ear oedema in mice, carrageenan-induced paw oedema in Swiss albino rats, cotton pellet-induced granuloma in rats and carrageenan-induced air pouch in mice were conducted. The IL-1b and TNF-a release from concanavalin A-stimulated monocytes was measured by ELISA. Results: Methanol extract of H. cheirifolia is rich in polyphenols and flavonoids. Cinnamic acid and rutin represent the major constituents. Methanol extract up to 2000 mg/kg did not produce any toxic effects. Topical application of 2 mg/ear of the extract produced 78.7% of inhibition on ear swilling. Oral pre-treatment of rats with 200 and 400 mg/kg of the extract inhibited paw oedema by 70% and 89%, respectively. At 200 mg/kg, granuloma dry and wet weights were reduced by 41.85% and 61.72%, respectively. Moreover, the treatment with methanol extract at 1 mg/kg exerted 62.7% of inhibition on leucocytes migrated into the ear pouch. TNF-a and IL-1b release was reduced by 69% and 78%, respectively, with 1 lg/mL of the extract. Conclusion: Methanol extract of H. cheirifolia possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.ARTICLE HISTORY

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Involvement of tumor necrosis factor (TNF)-α in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin edema in mice

Cited by 150 publications

References 21 publications

Caffeic Acid Reduces Cutaneous Tumor Necrosis Factor Alpha (TNF-α), IL-6 and IL-1β Levels and Ameliorates Skin Edema in Acute and Chronic Model of Cutaneous Inflammation in Mice

Caffeic Acid Reduces Cutaneous Tumor Necrosis Factor Alpha (TNF-α), IL-6 and IL-1β Levels and Ameliorates Skin Edema in Acute and Chronic Model of Cutaneous Inflammation in Mice

Learning from Both Sides: Experiences and Opportunities in the Investigation of Australian Aboriginal Medicinal Plants

Phytochemical screening and anti-inflammatory properties of AlgerianHertia cheirifoliamethanol extract

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