Involvement of TRPC1 in Nampt-induced cardiomyocyte hypertrophy through the activation of ER stress

Li, J.; Wu, Wenchao; Zhao, Mingyue; Liu, X.

doi:10.14715/cmb/2017.63.4.6

Cited by 3 publications

(2 citation statements)

References 22 publications

(30 reference statements)

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“…However, by overexpression of TRPC1, nicotinamide phosphoribosyl transferase was thought to induce cardiomyocyte hypertrophy by an ER stress-induced pathway. From this point of view, silencing of the TRPC1 gene may play a protective role in the prevention of cardiac hypertrophy [34]. In our study, ER stress and TRPC1 also increased significantly.…”

Section: Discussionsupporting

confidence: 64%

Investigation of the effect of hyperthyroidism on endoplasmic reticulum stress and tran- sient receptor potential canonical 1 channel in the kidney

et al. 2021

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Background/aim Hyperthyroidism is associated with results in increased glomerular filtration rate as well as increased renin-angiotensin-aldosterone activation. The disturbance of Ca 2+ homeostasis in the endoplasmic reticulum (ER) is associated with many diseases, including diabetic nephropathy and hyperthyroidism. Transient receptor potential canonical 1 (TRPC1) channel is the first cloned TRPC family protein. Although it is expressed in many places in the kidney, its function is uncertain. TRPC1 is involved in regulating Ca 2+ homeostasis, and its upregulation increases ER Ca 2+ level, activates the unfolded protein response, which leads to cellular damage in the kidney. This study investigated the role of TRPC1 in the kidneys of hyperthyroid rats in terms of ER stress markers that are glucose-regulated protein 78 (GRP78), activating transcription factor 6 (ATF6), (protein kinase R (PKR)-like endoplasmic reticulum kinase) (PERK), Inositol-requiring enzyme 1 (IRE1). Materials and methods Twenty male rats were assigned into control and hyperthyroid groups (n = 10). Hyperthyroidism was induced by adding 12 mg/L thyroxine into the drinking water of rats for 4 weeks. The serum-free T3 and T4 (fT3, fT4), TSH, blood urea nitrogen (BUN), and creatinine levels were measured. The histochemical analysis of kidney sections for morphological changes and also immunohistochemical and western blot analysis of kidney sections were performed for GRP78, ATF6, PERK, IRE1, TRPC1 antibodies. Results TSH, BUN, and creatinine levels decreased while fT3 and fT4 levels increased in the hyperthyroid rat. The morphologic analysis resulted in the capillary basal membrane thickening in glomeruli and also western blot, and immunohistochemical results showed an increase in TRPC1, GRP78, and ATF6 in the hyperthyroid rat (p < 0.05). Conclusion In conclusion, in our study, we showed for the first time that the relationship between ER stress and TRPC1, and their increased expression caused renal damage in hyperthyroid rats.

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Section: Discussionsupporting

confidence: 64%

Investigation of the effect of hyperthyroidism on endoplasmic reticulum stress and tran- sient receptor potential canonical 1 channel in the kidney

et al. 2021

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“…Also, it has been demonstrated that MEF2A is involved in the homeostasis of cardiac cells [53]. Studies show that NAMPT, possibly regulated by miR-548b-3p, is one of the major genes involved in hypertrophy of the heart [54]. Hypertrophy is known as a disease that can lead to IHD and other cardiovascular dysfunctions [55].…”

Section: Discussionmentioning

confidence: 99%

Serum miRNA Profile in Diabetic Patients with Ischemic Heart Disease as a Promising non-invasive Biomarker

Bielska¹,

Bauer²,

Szałkowska³

et al. 2020

Preprint

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Background The increasing morbidity and mortality of type 2 diabetic mellitus (T2DM) patients with ischemic heart disease (IHD) highlights an urgent need to identify early biomarkers, which would help to predict individual risk of development of IHD. Here, we postulate that circulating serum-derived miRNAs may serve as potential biomarkers for early IHD diagnosis and help to identify diabetic individuals with a predisposition to undergo IHD. Methods We obtained serum samples from T2DM patients either with IHD or IHD-free and analysed the expression levels of 798 miRNAs using the NanoString nCounter Technology Platform. The prediction of the putative miRNAs targets was performed using the Ingenuity Pathway Analysis (IPA) software. Receiver operating characteristic (ROC) analysis was used to assess the diagnostic value of identified miRNAs. Results Our data showed that 9 miRNAs (miR-1224-5p, miR-1303, miR-3147, miR-4455, miR-498, miR-548b-3p, miR-548d-3p, miR-615-3p, miR-651-5p) were significantly upregulated in T2DM IHD patients compared to T2DM patients without IHD. In patients with upregulated miRNA, functional enrichment analysis of target genes by IPA indicated networks and canonical pathways involved in the pathology of the cardiovascular system. All tested miRNAs showed high diagnostic value (AUC > 0.8). Conclusions Taken together, our findings suggest that circulating miRNAs might have a crucial role in the development of IHD in diabetic patients and may be used as a potential biomarker for early diagnosis.

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The molecular link between obesity and genomic instability in cancer development

An¹,

Pipia²,

Ruiz³

et al. 2023

Cancer Letters

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Involvement of TRPC1 in Nampt-induced cardiomyocyte hypertrophy through the activation of ER stress

Cited by 3 publications

References 22 publications

Investigation of the effect of hyperthyroidism on endoplasmic reticulum stress and tran- sient receptor potential canonical 1 channel in the kidney

Investigation of the effect of hyperthyroidism on endoplasmic reticulum stress and tran- sient receptor potential canonical 1 channel in the kidney

Serum miRNA Profile in Diabetic Patients with Ischemic Heart Disease as a Promising non-invasive Biomarker

The molecular link between obesity and genomic instability in cancer development

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