Involvement of Transient Receptor Potential Vanilloid Receptor 1, (TRPV1)-Expressing Vagal Nerve in the Inhibitory Effect of Gastric Acidification on Exogenous Motilin-Induced Gastric Phase III Contractions in Suncus murinus

Yoshimura, Masashi; Mikami, Takashi; Kuroda, Kayuri; Nishida, Maki; Ito, Kazuma; Mondal, Anupom; Koyama, Kuniyuki; Jogahara, Takamichi; Sakata, Ichiro; Sakai, Takafumi

doi:10.1007/s10620-015-4023-z

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…; Yoshimura et al. ). In brief, through a midline laparotomy, a strain gauge force transducer developed in our laboratory (Mondal et al.…”

Section: Methodsmentioning

confidence: 96%

“…; Yoshimura et al. ) was sutured to the dorsal portion of the lower gastric body and on the serosal surface of the duodenal body (3 cm distal to the antro‐pyloric junction) for measuring the circular muscle contractions. Waterproofing and response properties were checked in all transducers before implantation.…”

Section: Methodsmentioning

confidence: 99%

“…After fasting for 3-5 h, each animal was anesthetized with an intraperitoneal (IP) injection of the combination of midazolam (4 mg/kg; psychotropic; Sandoz K.K., Yamagata, Japan), Domitor (0.3 mg/kg; pain relief and suppression; Nippon Zenyaku Kogyo Co., Fukushima, Japan), and Vetorphale (5 mg/kg; pain relief after operation; Meiji Seika Pharma Co., Tokyo, Japan). The methodology of animal surgery, implantation of transducer, and recording of GI motility were followed as described previously Miyano et al 2013;Kuroda et al 2015;Yoshimura et al 2016). In brief, through a midline laparotomy, a strain gauge force transducer developed in our laboratory Miyano et al 2013;Kuroda et al 2015;Yoshimura et al 2016) was sutured to the dorsal portion of the lower gastric body and on the serosal surface of the duodenal body (3 cm distal to the antro-pyloric junction) for measuring the circular muscle contractions.…”

Section: Animal Surgery For Gi Motility Recording In Vivomentioning

confidence: 99%

“…The methodology of animal surgery, implantation of transducer, and recording of GI motility were followed as described previously Miyano et al 2013;Kuroda et al 2015;Yoshimura et al 2016). In brief, through a midline laparotomy, a strain gauge force transducer developed in our laboratory Miyano et al 2013;Kuroda et al 2015;Yoshimura et al 2016) was sutured to the dorsal portion of the lower gastric body and on the serosal surface of the duodenal body (3 cm distal to the antro-pyloric junction) for measuring the circular muscle contractions. Waterproofing and response properties were checked in all transducers before implantation.…”

Section: Animal Surgery For Gi Motility Recording In Vivomentioning

confidence: 99%

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Underlying mechanism of the cyclic migrating motor complex inSuncus murinus: a change in gastrointestinal pH is the key regulator

et al. 2017

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In the fasted gastrointestinal (GI) tract, a characteristic cyclical rhythmic migrating motor complex (MMC) occurs in an ultradian rhythm, at 90–120 min time intervals, in many species. However, the underlying mechanism directing this ultradian rhythmic MMC pattern is yet to be completely elucidated. Therefore, this study aimed to identify the possible causes or factors that involve in the occurrence of the fasting gastric contractions by using Suncus murinus a small model animal featuring almost the same rhythmic MMC as that found in humans and dogs. We observed that either intraduodenal infusion of saline at pH 8 evoked the strong gastric contraction or continuously lowering duodenal pH to 3‐evoked gastric phase II‐like and phase III‐like contractions, and both strong contractions were essentially abolished by the intravenous administration of MA 2029 (motilin receptor antagonist) and D‐Lys3‐GHRP6 (ghrelin receptor antagonist) in a vagus‐independent manner. Moreover, we observed that the prostaglandin E2‐alpha (PGE2‐ α) and serotonin type 4 (5HT4) receptors play important roles as intermediate molecules in changes in GI pH and motilin release. These results suggest a clear insight mechanism that change in the duodenal pH to alkaline condition is an essential factor for stimulating the endogenous release of motilin and governs the fasting MMC in a vagus‐independent manner. Finally, we believe that the changes in duodenal pH triggered by flowing gastric acid and the release of duodenal bicarbonate through the involvement of PGE2‐ α and 5HT4 receptor are the key events in the occurrence of the MMC.

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“…; Yoshimura et al. ). In brief, through a midline laparotomy, a strain gauge force transducer developed in our laboratory (Mondal et al.…”

Section: Methodsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Animal Surgery For Gi Motility Recording In Vivomentioning

confidence: 99%

Section: Animal Surgery For Gi Motility Recording In Vivomentioning

confidence: 99%

See 2 more Smart Citations

Underlying mechanism of the cyclic migrating motor complex inSuncus murinus: a change in gastrointestinal pH is the key regulator

et al. 2017

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“…Phase III contractions of MMC propagate from the stomach to the distal small intestine. The MMC is considered to have an important role in preparing for the next meal by stimulating mechanical and chemical cleaning of the GI tract . In addition, it has been thought that MMCs can also prevent bacterial overgrowth of the small intestine …”

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confidence: 99%

Study of termination of postprandial gastric contractions in humans, dogs and Suncus murinus: role of motilin‐ and ghrelin‐induced strong contraction

et al. 2017

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Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats

et al. 2018

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The TRPV1 channel is activated in neurons by capsaicin, oxidative stress, acidic pH and heat factors, and these factors are attenuated by the antioxidant role of calorie restriction (CR). Hence, we investigated the hypothesis that the antioxidant roles of CR and food frequency (FF) may modulate TRPV1 activity and apoptosis through inhibition of mitochondrial oxidative stress in hippocampal (HIPPON) and dorsal root ganglion neurons (DRGN). We investigated the contribution of FF and CR to neuronal injury and apoptosis through inhibition of TRPV1 in rats. We assigned rats to control, FF and FF + CR groups. A fixed amount of food ad libitum was supplemented to the control and FF groups for 20 weeks, respectively. FF + CR group were fed the same amount of food as the control group but with 20% less calories during the same period. In major results, TRPV1 currents, intracellular Ca levels, apoptosis, reactive oxygen species, mitochondrial depolarization, PARP-1 expression, caspase 3 and 9 activity and expression values were found to be increased in the HIPPON and DRGN following FF treatment, and these effects were decreased following FF + CR treatment. The FF-induced decrease in cell viability of HIPPO and DRGN, and vitamin E concentration of brain, glutathione peroxidase, vitamin A, and β-carotene values of the HIPPO, DRGN, plasma, liver and kidney were increased by FF + DR treatment, although lipid peroxidation levels in the same samples were decreased. In conclusion, CR reduces FF-induced increase of oxidative stress, apoptosis and Ca entry through TRPV1 in the HIPPON and DRGN. Our findings may be relevant to the etiology and treatment of obesity following CR treatment.

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Involvement of Transient Receptor Potential Vanilloid Receptor 1, (TRPV1)-Expressing Vagal Nerve in the Inhibitory Effect of Gastric Acidification on Exogenous Motilin-Induced Gastric Phase III Contractions in Suncus murinus

Abstract: Gastric acidification in S. murinus inhibits motilin-induced contractions, a finding similar to results observed in humans, while TRPV1-expressing vagus nerves play a role in the inhibitory mechanism.

Cited by 8 publications

References 45 publications

Underlying mechanism of the cyclic migrating motor complex inSuncus murinus: a change in gastrointestinal pH is the key regulator

Underlying mechanism of the cyclic migrating motor complex inSuncus murinus: a change in gastrointestinal pH is the key regulator

Study of termination of postprandial gastric contractions in humans, dogs and Suncus murinus: role of motilin‐ and ghrelin‐induced strong contraction

Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats

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