Involvement of TNF α, IL-1β and IL-1 Receptor Antagonist in LPS-induced Rabbit Uveitis

Mo, Jun; Matsukawa, Akihiro; Ohkawara, Susumu; Yoshinaga, Masaru

doi:10.1006/exer.1997.0451

Cited by 47 publications

(40 citation statements)

References 29 publications

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“…The amounts of anti-TNFα and rrIL-1ra (10 µg each) used have been shown to suppress the biologic effects of these cytokines. Ten µg of anti-TNFα mAb significantly inhibited neutrophil infiltration, protein leakage and IL-8 production, and an increase in the amount (50 µg) did not further increase the inhibition (Mo et al, 1998). Similarly, 10 µg of rrIL-1ra inhibited significantly leukocyte infiltration but not protein leakage.…”

Section: Discussionmentioning

confidence: 64%

“…Similarly, 10 µg of rrIL-1ra inhibited significantly leukocyte infiltration but not protein leakage. Further increase in the amount of rrIL-1ra (50 µg) did not increase inhibition of leukocyte infiltration in the same model of LPS-induced uveitis in rabbits (Mo et al, 1998). Interestingly, we previously observed that the 12 hr aqueous IL-8 levels were inhibited by treatment with anti-TNFα mAb but not by rrIL-1Ra, and rrIL-1Ra did not increase the anti-TNFα mAb-caused inhibition (Mo et al, 1999), indicating that IL-1 in the early stage of the uveitis did not stimulate IL-8 production.…”

Section: Discussionmentioning

confidence: 83%

“…Combination of anti-TNFα mAb and rrIL-1Ra suppressed 93 % of the GRO levels, suggesting that TNFα and IL-1, which do not attract neutrophils directly (Mrowietz, Schroder and Christophers, 1988 ;Yoshimura et al, 1987), mediate the neutrophil infiltration (Mo et al, 1998) through GRO in LPSinduced uveitis.…”

Section: Discussionmentioning

confidence: 92%

“…Leukocyte counts and protein and IL-8 levels in the aqueous humor were determined 24 hr later since previous studies have shown that both leukocyte infiltration and protein leakage in LPS-induced uveitis in rabbits peak at 24 hr following injection of LPS (Mo et al, 1998).…”

Section: Effects Of Anti-gro Serum On Lps-induced Leukocyte Infiltratmentioning

confidence: 99%

“…Neutrophils are the major cellular population of infiltrating leukocytes in anterior uveitis induced by intravitreal injection of lipopolysaccharide (LPS) in rabbits (Howes et al, 1985 ;Mo et al, 1998). CXC chemokines having ELR motif (Cole et al, 1998) are believed to mediate neutrophil infiltration during ocular inflammation.…”

Section: Introductionmentioning

confidence: 99%

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CXC Chemokine GRO is Essential for Neutrophil Infiltration in LPS-induced Uveitis in Rabbits

Matsukawa

Ohkawara

et al. 2000

Experimental Eye Research

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 92%

Section: Effects Of Anti-gro Serum On Lps-induced Leukocyte Infiltratmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CXC Chemokine GRO is Essential for Neutrophil Infiltration in LPS-induced Uveitis in Rabbits

Matsukawa

Ohkawara

et al. 2000

Experimental Eye Research

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Infliximab Therapy for the Treatment of Refractory Ocular Inflammatory Disease

Sobrin

Kim²,

Christen³

et al. 2007

Arch Ophthalmol

129

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To report the outcomes of infliximab therapy in the treatment of ocular inflammatory disease refractory to traditional immunomodulatory therapy (IMT). Methods:We retrospectively reviewed the medical records of 27 patients. All patients had noninfectious ocular inflammatory disease refractory to traditional IMT and received 5 mg/kg of infliximab at 2-week to 8-week intervals. Main outcome measures were clinical response, reduction in concomitant IMT, and adverse effects. Cumulative incidences of inflammation control and vision change were calculated using life-table methods.Results: Twenty-one patients experienced sustained improvement in inflammation with their initial course of infliximab therapy. Cumulative incidence of inflammation resolution at 12 months was greater than 90%. Sixteen patients were able to decrease the dose of their concomitant IMT medication or stop all other IMT. Four patients were able to discontinue all other IMT while receiving infliximab therapy. Three patients with scleritis were eventually able to remain inflammation-free while not taking any medication. At 12 months, 56% and 65% of left and right eyes, respectively, showed visual acuity improvement by 2 or more Snellen lines. Only 1 patient developed an adverse event requiring therapy discontinuation. Conclusions:We found a high rate of ocular inflammation control with infliximab therapy. The incidence of adverse effects in this study was low.

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Immune privilege persists in eyes with extreme inflammation induced by intravitreal LPS

Streilein

2001

Eur. J. Immunol.

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Since immune privilege is believed to exist in the eye in order to suppress sight‐destroying inflammation, we wondered whether eyes with intraocular inflammation retain the immune privileged state. Intraocular inflammation was induced by injection of lipopolysaccharide (LPS) into the vitreous cavity of BALB / c mouse eyes, which showed a peak in intensity at approximately 9 h. At this time point, inflamed eyes were examined for their capacity to afford immune privilege to injected allogeneic tumor cells, and to promote anterior chamber‐associated immune deviation (ACAID) to antigens injected locally. In addition, aqueous humor (AqH) harvested from inflamed eyes was tested for its ability to suppress T cell activation. Surprisingly, eyes with acute, intense intraocular inflammation allowed allogeneic tumor cells to form progressively growing tumors, and these same eyes promoted ACAID. Moreover, AqH harvested from inflamed eyes strongly inhibited T cell activation. We conclude that the type of extreme, intraocular inflammation evoked by intravitreally injected LPS fails to abolish immune privilege in the eye. These findings are discussed in light of the effects of other types of inflammation on the integrity of ocular immune privilege, and with respect to the capacity of the eye to maintain immune privilege by more than one mechanism.

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Involvement of TNF α, IL-1β and IL-1 Receptor Antagonist in LPS-induced Rabbit Uveitis

Cited by 47 publications

References 29 publications

CXC Chemokine GRO is Essential for Neutrophil Infiltration in LPS-induced Uveitis in Rabbits

CXC Chemokine GRO is Essential for Neutrophil Infiltration in LPS-induced Uveitis in Rabbits

Infliximab Therapy for the Treatment of Refractory Ocular Inflammatory Disease

Immune privilege persists in eyes with extreme inflammation induced by intravitreal LPS

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