Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance

Shen, Xiao-Min; Liao, Chun Yen; Lu, Xue‐Ping; Wang, Zhe; Wang, Jinjun; Dou, Wei

doi:10.3390/ijms17081361

Cited by 22 publications

(20 citation statements)

References 51 publications

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“…1C) methods were developed and proven to be successful in T. urticae, 27,28,30 T. cinnabarinus 22,[24][25][26] and P. citri. [15][16][17][18] Leaf disc (leaf coating)-and immersion (soaking)-mediated RNAi ( Fig. 1A and B) in these piercing-sucking mites has been proved to be functional in laboratory conditions.…”

Section: Double-stranded Rna Delivery (Fig 1 and Table 1)mentioning

confidence: 97%

“…A) and stem leaf‐mediated (Fig. C) methods were developed and proven to be successful in T. urticae , T. cinnabarinus and P. citri . Leaf disc (leaf coating)‐ and immersion (soaking)‐mediated RNAi (Fig.…”

Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

“…Leaf disc‐based dsRNA feeding led to an inhibition of 65–81% in the expression of detoxification‐related genes in P. citri (e.g. mu‐class glutathione S‐transferase and carboxylesterase ), and this in turn resulted in increased susceptibility to acaricides . Through silencing of two essential development‐related genes, chitinase (involved in chitin degradation and reconstruction) and Spook (a Halloween ecdysteroid hormone‐synthesizing gene), mortality rates of 80.1 and 40%, respectively, at 72 h after the feeding of dsRNA could be observed in P. citri mites as a consequence of failure of molting and the mites being trapped in the old cuticle .…”

Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

“…22,24 As expected, T. cinnabarinus fed on cotton plants expressing dsRNA against a P450 gene CYP392A4 showed a 43% decrease in target gene expression, and this resulted in reduced reproductive ability of the mites. 16 Use of transgenic plants with chloroplasts expressing dsRNA is a promising strategy for efficient RNAi-mediated crop protection against insect pests, 7 and this strategy might also be useful for spider mite control as they directly feed on plant cells. Leaf disc-based dsRNA feeding led to an inhibition of 65-81% in the expression of detoxification-related genes in P. citri (e.g.…”

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

“…Leaf-mediated dsRNA feeding (48 h) Increased mortality caused by fenpropathrin 16 Chitinase-1 (60%) Reduced mortality rate of bees 34 wileyonlinelibrary.com/journal/ps turn resulted in increased susceptibility to acaricides. 15,16 Through silencing of two essential development-related genes, chitinase (involved in chitin degradation and reconstruction) and Spook (a Halloween ecdysteroid hormone-synthesizing gene), mortality rates of 80.1 and 40%, respectively, at 72 h after the feeding of dsRNA could be observed in P. citri mites as a consequence of failure of molting and the mites being trapped in the old cuticle. 17,18 The tested RNAi targets in mites, listed in Table 1, could be further evaluated as candidates for mite control, especially those resulting in high mortality (coatomer subunit epsilon genes, chitinase, etc.)…”

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

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Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

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Mites comprise a group of key agricultural pests on a wide range of crops. They cause harm through feeding on the plant and transferring dangerous pathogens, and the rapid evolution of pesticide resistance in mites highlights the need for novel control methods. Currently, RNA interference (RNAi) shows great potential for insect pest control. Here, we review the literature regarding RNAi in mite pests. We discuss different target genes and RNAi efficiency in various mite species, a promising Varroa control program using RNAi, the synergy of RNAi with plant defense mechanisms and microorganisms, and current understanding of systemic movement of double-stranded RNA (dsRNA). On the basis of this evidence, we can conclude that there is clear potential for application of RNAi-based mite control, but further research on several aspects of RNAi in mites is needed, including: (i) the factors influencing RNAi efficiency, (ii) the mechanism of environmental RNAi and cross-kingdom dsRNA trafficking, (iii) the mechanism of possible systemic and parental RNAi, and (iv) non-target effects, specifically in predatory mites, which should be considered during RNAi target selection. © 2018 Society of Chemical Industry.

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Section: Double-stranded Rna Delivery (Fig 1 and Table 1)mentioning

confidence: 97%

Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

Section: Efficiency Of Rna In Various Mite Speciesmentioning

confidence: 99%

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

Section: Gene Silencing and Phenotypic Effects Induced By Dsrna In MImentioning

confidence: 99%

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Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

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From Conventional Lewis Acids to Heterogeneous Montmorillonite K10: Eco‐Friendly Plant‐Based Catalysts Used as Green Lewis Acids

et al. 2018

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The concept of green chemistry began in the USA in the 1990s. Since the publication of the 12 principles of this concept, many reactions in organic chemistry have been developed, and chemical products have been synthesized under environmentally friendly conditions. Lewis acid mediated synthetic transformations are by far the most numerous and best studied. However, the use of certain Lewis acids may cause risks to environmental and human health. This Review discusses the evolution of Lewis acid catalyzed reactions from a homogeneous liquid phase to the solid phase to yield the expected organic molecules under green, safe conditions. In particular, recent developments and applications of biosourced catalysts from plants are highlighted.

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Functional analysis of an upregulated calmodulin gene related to the acaricidal activity of curcumin against Tetranychus cinnabarinus (Boisduval)

Zhou

Guo

Luo

et al. 2020

Pest Management Science

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BACKGROUND Curcumin is a promising botanical acaricidal compound with activity against Tetranychus cinnabarinus. Calmodulin (CaM) is a key calcium ion (Ca2+) sensor that plays a vital role in calcium signaling. Overexpression of the CaM gene with inducible character occurs in curcumin‐treated mites, but its functional role remains to be further analyzed by RNA interference (RNAi) and protein expression. RESULTS A CaM gene was cloned from T. cinnabarinus (designated TcCaM). TcCaM was upregulated and the protein was activated in mites by curcumin. The susceptibility of mites to curcumin was decreased after inhibiting CaM function with anti‐CaM drug trifluoperazine (TFP) and silencing CaM transcription with RNAi, suggesting that the CaM gene is involved in the acaricidal activity of curcumin against mites. Moreover, the TFP pre‐treated Sf9 cells were resistant to curcumin‐mediated increase in [Ca2+]i levels, indicating that CaM‐mediated Ca2+ homeostasis was disturbed by curcumin. TcCaM was then re‐engineered for heterologous expression in Escherichia coli. Strikingly, our results showed that the recombinant CaM protein was directly activated by curcumin via inducing its conformational changes, its half‐maximal effective concentration (EC50) value is 0.3 μmol L‐1 in vitro, which is similar to curcumin against CaM‐expressing Sf9 cells (0.76 μmol L‐1) in vivo. CONCLUSION These results confirm that the overexpressed CaM gene is involved in the acaricidal activity of curcumin, and the mode of action of curcumin may be via activating CaM function, and thereby disrupting Ca2+ homeostasis in T. cinnabarinus. This study highlights the novel target mechanism of new acaricides, promoting our understanding of the molecular mechanism of CaM‐mediated acaricide targets in mites.

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Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance

Cited by 22 publications

References 51 publications

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

From Conventional Lewis Acids to Heterogeneous Montmorillonite K10: Eco‐Friendly Plant‐Based Catalysts Used as Green Lewis Acids

Functional analysis of an upregulated calmodulin gene related to the acaricidal activity of curcumin against Tetranychus cinnabarinus (Boisduval)

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