Involvement of the Skeletal Renin-Angiotensin System in Age-Related Osteoporosis of Ageing Mice

Renin inhibitor aliskiren exerts beneficial effect on trabecular bone by regulating skeletal renin-angiotensin system and kallikrein-kinin system in ovariectomized mice

“…Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control was used to normalize the data to determine the relative expression of the target genes. The PCR primers used in this study were as previously described [16,17,29].…”

“…Functional studies revealed that Ang II could stimulate the differentiation and activity of osteoclasts in vivo [14] and in vitro [15] and aggravate the loss of bone minerals in rats with osteoporosis induced by estrogen deficiency [14]; furthermore, the AT1R knockout mice showed high bone mass [16]. In addition, we recently demonstrated that the local RAS in bone was involved in age-related osteoporosis of aging mice [17], bone deteriorations of mice with either obstructive nephropathy [18] or type 1 diabetes [19], and others elucidated the involvement of skeletal RAS in the process of fracture healing in a mouse femur fracture model [20] and the steroid-induced osteonecrosis in rabbits [21] as well as the development of postmenopausal osteoporosis in ovariectomized (OVX) animal models [14,22]. Therefore, the emerging evidences demonstrated that the local RAS displays important biological actions in bone tissue.…”

Involvement of Renin–Angiotensin System in Damage of Angiotensin-Converting Enzyme Inhibitor Captopril on Bone of Normal Mice

“…6) Recent in vivo studies showed that the components of RAS, such as renin, angiotensin-converting enzyme (ACE), and angiotensin II (Ang II) receptors, were expressed in the local milieu of bone, [7][8][9][10] and in vitro study identified the expression of Ang II receptors in primary osteoblasts derived from newborn mouse calvaria, 7) indicating the components of RAS are expressed locally in bone microenvironment. Our further animal studies demonstrated that the local RAS in bone was involved in age-related osteoporosis of aging mice, 11) and bone deteriorations of mice with either obstructive nephropathy 12) or type 1 diabetes. 13) Other groups elucidated the involvement of skeletal RAS in the process of fracture healing in a mouse femur fracture model, 14) and the steroid-induced osteonecrosis in rabbits 10) as well as the development of postmenopausal osteoporosis in ovariectomized (OVX) animal models 15,16) and glucocorticoid-induced osteoporosis.…”

Renin Inhibition Improves Ovariectomy-Induced Osteoporosis of Lumbar Vertebra in Mice

“…13) In addition, we recently demonstrated that the local RAS in bone was involved in age-related osteoporosis of aging mice, 14) bone deteriorations of mice with either obstructive nephropathy 15) or type 1 diabetes, 16) and others elucidated the involvement of skeletal RAS in the process of fracture healing in a mouse femur fracture model. 17) Therefore, the local RAS displays important biological actions in bone tissue.…”

“…9,10) Functional studies revealed that ANG II could stimulate the differentiation and activity of osteoclasts in vivo 11) and in vitro, 12) and aggravate the loss of bone minerals in rats with osteoporosis induced by estrogen deficiency, 11) furthermore, the ANG II type 1 receptor knockout mice showed high bone mass. 13) In addition, we recently demonstrated that the local RAS in bone was involved in age-related osteoporosis of aging mice, 14) bone deteriorations of mice with either obstructive nephropathy 15) or type 1 diabetes, 16) and others elucidated the involvement of skeletal RAS in the process of fracture healing in a mouse femur fracture model. 17) Therefore, the local RAS displays important biological actions in bone tissue.…”