Involvement of the Cell Wall–Integrity Pathway in Signal Recognition, Cell-Wall Biosynthesis, and Virulence in <i>Magnaporthe oryzae</i>

Lu, Kailun; Chen, Rangrang; Yang, Yi; Xu, Hui; Ji-hong, Jiang; Li, Lianwei

doi:10.1094/mpmi-11-22-0231-cr

Cited by 6 publications

(3 citation statements)

References 147 publications

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“…Loss of ceramide by deletion of MoLag1 led to a significantly reduced phosphorylation of Mps1. Interestingly, exogenous treatment with ceramide partially restored Mps1 phosphorylation in the Molag1 mutant [51,87]. These results suggest that ceramide regulates the PKC-MAPK pathway and cell wall integrity in M. oryzae.…”

Section: The Cross-talk Between the Sphingolipid Pathway And Other Pa...mentioning

confidence: 68%

The biological functions of sphingolipids in plant pathogenic fungi

Zhu,

Li,

Bao

et al. 2023

PLoS Pathog

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Sphingolipids are critically significant in a range of biological processes in animals, plants, and fungi. In mammalian cells, they serve as vital components of the plasma membrane (PM) in maintaining its structure, tension, and fluidity. They also play a key role in a wide variety of biological processes, such as intracellular signal transduction, cell polarization, differentiation, and migration. In plants, sphingolipids are important for cell development and for cell response to environmental stresses. In pathogenic fungi, sphingolipids are crucial for the initiation and the development of infection processes afflicting humans. However, our knowledge on the metabolism and function of the sphingolipid metabolic pathway of pathogenic fungi affecting plants is still very limited. In this review, we discuss recent developments on sphingolipid pathways of plant pathogenic fungi, highlighting their uniqueness and similarity with plants and animals. In addition, we discuss recent advances in the research and development of fungal-targeted inhibitors of the sphingolipid pathway, to gain insights on how we can better control the infection process occurring in plants to prevent or/and to treat fungal infections in crops.

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Section: The Cross-talk Between the Sphingolipid Pathway And Other Pa...mentioning

confidence: 68%

The biological functions of sphingolipids in plant pathogenic fungi

Zhu,

Li,

Bao

et al. 2023

PLoS Pathog

View full text Add to dashboard Cite

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“…The fungal cell wall is a critical structural component primarily composed of chitin, glucan, chitosan, and cellulose, and it plays a vital role in maintaining cell morphology and integrity ( Lu et al, 2023 ). Congo Red, a fungal cell wall inhibitor, specifically binds to β-1,3-glucans, disrupting cell wall assembly and inhibiting fungal growth ( Ram and Klis, 2006 ; Liu Z. et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

StRAB4 gene is required for filamentous growth, conidial development, and pathogenicity in Setosphaeria turcica

Li,

Zhu,

Wang

et al. 2024

Front. Microbiol.

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Setosphaeria turcica, the fungal pathogen responsible for northern corn leaf blight in maize, forms specialized infectious structures called appressoria that are critical for fungal penetration of maize epidermal cells. The Rab family of proteins play a crucial role in the growth, development, and pathogenesis of many eukaryotic species. Rab4, in particular, is a key regulator of endocytosis and vesicle trafficking, essential for filamentous growth and successful infection by other fungal pathogens. In this study, we silenced StRAB4 in S. turcica to gain a better understanding the function of Rab4 in this plant pathogen. Phenotypically, the mutants exhibited a reduced growth rate, a significant decline in conidia production, and an abnormal conidial morphology. These phenotypes indicate that StRab4 plays an instrumental role in regulating mycelial growth and conidial development in S. turcica. Further investigations revealed that StRab4 is a positive regulator of cell wall integrity and melanin secretion. Functional enrichment analysis of differentially expressed genes highlighted primary enrichments in peroxisome pathways, oxidoreductase and catalytic activities, membrane components, and cell wall organization processes. Collectively, our findings emphasize the significant role of StRab4 in S. turcica infection and pathogenicity in maize and provide valuable insights into fungal behavior and disease mechanisms.

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“…Thus, it is urgently required to develop a safer, more efficient and more reliable antifungal strategies to control rice blast fungus. The cell wall is the outer barrier of M. oryzae to resist external stress, including inappropriate pH, osmotic stress, temperature changes, oxidative stress and antifungal drugs, moreover, it enables to maintain cellular integrity and viability ( Lu K. et al, 2023 ). Thus, overcoming the fungal outer barrier is crucial in the development of biocontrol strategies to prevent pathogenic infection.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of a novel endo-β-1,6-glucanase MoGlu16 and its application in detecting cell wall β-1,6-glucan of Magnaporthe oryzae

Wang,

Li,

et al. 2024

Front. Microbiol.

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As an essential component of the fungal cell wall, β-1,6-glucan has an important role in the growth and development of fungi, but its distribution has not been investigated in Magnaporthe oryzae. Here, a novel β-1,6-glucanase from M. oryzae, MoGlu16, was cloned and expressed in Pichia pastoris. The enzyme was highly active on pustulan, with a specific activity of 219.0 U/mg at pH 5.0 and 50°C, and showed great selectivity for continuous β-1,6-glycosidic bonding polysaccharides. Based on this, β-1,6-glucan was selectively visualized in the vegetative hyphae, conidia and bud tubes of M. oryzae using a hydrolytically inactive GFP-tagged MoGlu16 with point mutations at the catalytic position (His-MoGlu16E236A-Gfp). The spore germination and appressorium formation were significantly inhibited after incubation of 105/ml conidia with 0.03 μg/μl MoGlu16. Mycelia treated with MoGlu16 produced reactive oxygen species and triggered the cell wall integrity pathway, increasing the expression levels of genes involved in cell wall polysaccharide synthesis. These results revealed that MoGlu16 participated in the remodeling of cell wall in M. oryzae, laying a foundation for the analysis of cell wall structure.

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Involvement of the Cell Wall–Integrity Pathway in Signal Recognition, Cell-Wall Biosynthesis, and Virulence in Magnaporthe oryzae

Cited by 6 publications

References 147 publications

The biological functions of sphingolipids in plant pathogenic fungi

The biological functions of sphingolipids in plant pathogenic fungi

StRAB4 gene is required for filamentous growth, conidial development, and pathogenicity in Setosphaeria turcica

Functional analysis of a novel endo-β-1,6-glucanase MoGlu16 and its application in detecting cell wall β-1,6-glucan of Magnaporthe oryzae

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