Involvement of the Actinobacillus pleuropneumoniae ompW Gene in Confrontation of Environmental Pressure

Chen, Xiabing; Shao, Zhiyong; Wu, Lijun; He, Bin; Yang, Wenhai; Chen, Jie; Jin, Erguang; Huang, Qi; Lei, Liancheng; Xu, Jiajia; Li, Haotian; Zhang, Hui; Wan, Yun; Liu, Wu; Zhou, Rui

doi:10.3389/fvets.2022.846322

Cited by 3 publications

(3 citation statements)

References 49 publications

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“…Among these subtypes, serovars 1 and 5 are generally considered highly virulent strains whereas serovars 2 and 7 are attenuated strains (Chen et al. 2022 ). However, the current study found that serovar 15 is predominant (44.7%), followed by highly virulent (serovar 1 and 5, 31.6%) and attenuated strains (serovar 2 and 7, 23.7%).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis

Ke,

Lai,

Hsu

et al. 2024

Veterinary Quarterly

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Actinobacillus pleuropneumoniae infection causes a high mortality rate in porcine animals. Antimicrobial resistance poses global threats to public health. The current study aimed to determine the antimicrobial susceptibilities and probe the resistome of A. pleuropneumoniae in Taiwan. Herein, 133 isolates were retrospectively collected; upon initial screening, 38 samples were subjected to next-generation sequencing (NGS). Over the period 2017–2022, the lowest frequencies of resistant isolates were found for ceftiofur, cephalexin, cephalothin, and enrofloxacin, while the highest frequencies of resistant isolates were found for oxytetracycline, streptomycin, doxycycline, ampicillin, amoxicillin, kanamycin, and florfenicol. Furthermore, most isolates (71.4%) showed multiple drug resistance. NGS-based resistome analysis revealed aminoglycoside- and tetracycline-related genes at the highest prevalence, followed by genes related to beta-lactam, sulfamethoxazole, florphenicol, and macrolide. A plasmid replicon (repUS47) and insertion sequences (IS10R and ISVAp11) were identified in resistant isolates. Notably, the multiple resistance roles of the insertion sequence IS10R were widely proposed in human medicine; however, this is the first time IS10R has been reported in veterinary medicine. Concordance analysis revealed a high consistency of phenotypic and genotypic susceptibility to florphenicol, tilmicosin, doxycycline, and oxytetracycline. The current study reports the antimicrobial characterization of A. pleuropneumoniae for the first time in Taiwan using NGS.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis

Ke,

Lai,

Hsu

et al. 2024

Veterinary Quarterly

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“…Eight-stranded β-barrel with a hydrophobic channel [86] Nalidixic acid [81]; kanamycin, colistin/carbapenem, and ceftriaxone [86,88]; ampicillin, tetracycline, and ceftriaxone [90]; enrofloxacin [91]; tobramycin [92]; ciprofloxacin and imipenem [93]; colicin S4, chlortetracycline, neomycin, and ampicillin [94][95][96]; methyl viologen [97]; and penicillin, kanamycin, and polymyxin B [98].…”

Section: Ompwmentioning

confidence: 99%

“…The methyl viologen sensitivity of ΔompW of S. typhimurium is 2.5 times greater than the WT [ 97 ]. The loss of OmpW in Actinobacillus pleuropneumoniae affects bacterial susceptibility to penicillin, kanamycin, and polymyxin B [ 98 ]. Meanwhile, the ompW of E. coli is also involved in the ethidium multidrug resistance gene E ( emrE )-mediated substrate efflux process and is mechanistically connected to EmrE [ 99 ].…”

Section: Ompwmentioning

confidence: 99%

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Zhou

Wang

et al. 2023

Microorganisms

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Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), which is home to several different outer membrane proteins (Omps). One class of important Omps is porins, which mediate the inflow of nutrients and several antimicrobial drugs. The microorganism’s sensitivity to antibiotics, which are predominantly targeted at internal sites, is greatly influenced by the permeability characteristics of porins. In this review, the properties and interactions of five common porins, OmpA, OmpC, OmpF, OmpW, and OmpX, in connection to porin-mediated permeability are outlined. Meanwhile, this review also highlighted the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through porins. Taken together, uncovering porins’ functional properties will pave the way to investigate effective agents or approaches that use porins as targets to get rid of resistant gram-negative bacteria.

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Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review

Tajer,

Paillart,

Dib

et al. 2024

Microorganisms

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Antimicrobial resistance (AMR) poses a serious global health concern, resulting in a significant number of deaths annually due to infections that are resistant to treatment. Amidst this crisis, antimicrobial peptides (AMPs) have emerged as promising alternatives to conventional antibiotics (ATBs). These cationic peptides, naturally produced by all kingdoms of life, play a crucial role in the innate immune system of multicellular organisms and in bacterial interspecies competition by exhibiting broad-spectrum activity against bacteria, fungi, viruses, and parasites. AMPs target bacterial pathogens through multiple mechanisms, most importantly by disrupting their membranes, leading to cell lysis. However, bacterial resistance to host AMPs has emerged due to a slow co-evolutionary process between microorganisms and their hosts. Alarmingly, the development of resistance to last-resort AMPs in the treatment of MDR infections, such as colistin, is attributed to the misuse of this peptide and the high rate of horizontal genetic transfer of the corresponding resistance genes. AMP-resistant bacteria employ diverse mechanisms, including but not limited to proteolytic degradation, extracellular trapping and inactivation, active efflux, as well as complex modifications in bacterial cell wall and membrane structures. This review comprehensively examines all constitutive and inducible molecular resistance mechanisms to AMPs supported by experimental evidence described to date in bacterial pathogens. We also explore the specificity of these mechanisms toward structurally diverse AMPs to broaden and enhance their potential in developing and applying them as therapeutics for MDR bacteria. Additionally, we provide insights into the significance of AMP resistance within the context of host–pathogen interactions.

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Involvement of the Actinobacillus pleuropneumoniae ompW Gene in Confrontation of Environmental Pressure

Cited by 3 publications

References 49 publications

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review

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