Involvement of superoxide dismutase in oxidative stress in the oriental fruit fly,<i>Bactrocera dorsalis</i>: molecular cloning and expression profiles

Gao, Xuemeng; Fu, Jia; Shen, Guangmao; Jiang, Hua-Qin; Dou, Wei; Wang, Jinjun

doi:10.1002/ps.3503

Cited by 33 publications

(16 citation statements)

References 67 publications

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“…On the other hand, Sod2 gene expression was attenuated suggesting lower mitochondrial metabolic activity. Our results confirm that different isoforms of SOD could respond to pollution derived stress in a different manner depending on both doses and combinations of heavy elements to which it is exposed (Tang et al, 2012;Gao et al, 2013;Won et al, 2014).…”

Section: Archives Of Insect Biochemistry and Physiologysupporting

confidence: 78%

“…Hydrogen peroxide is decomposed to water and oxygen by the action of catalase (CAT). It was observed that gene expression and activity of antioxidative enzymes changes as a response to various environmental stresses both in vertebrates (Hansen et al, 2006) and invertebrates (Roh et al, 2006;Vlahogianni et al, 2007), including insects (Tang et al, 2012;Gao et al, 2013;Won et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee

Nikolić

Purać

Orčić

et al. 2015

Arch Insect Biochem Physiol

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Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajača, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.

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Section: Archives Of Insect Biochemistry and Physiologysupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee

Nikolić

Purać

Orčić

et al. 2015

Arch Insect Biochem Physiol

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“…Notably, expression of SOD2 was sharply increased under cold stress but was reduced under heat stress. It may be that SOD2 (MnSOD), which is located in the mitochondria, has a specific response to various degrees of destruction of mitochondria induced by temperature stress (Dawson et al, 2015;Gao et al, 2013). Overall, the antioxidant enzyme system in Q. spinosa was activated and could protect the liver from the thermal stress-induced oxidative stress (Meng et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Liu

et al. 2018

Journal of Experimental Biology

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Ambient temperature-associated stress can affect normal physiological functions in ectotherms. To assess the effects of cold or heat stress on amphibians, giant spiny frogs () were acclimated at 22°C followed by exposure to 5°C or 30°C for 0, 3, 6, 12, 24 and 48 h, respectively. Histological alterations, apoptotic index, generation of mitochondrial reactive oxygen species (ROS), antioxidant activity indices and stress-response gene expression in frog livers were subsequently determined. Results showed that many fat droplets appeared after 12 h of heat stress and the percentage of melanomacrophage centres significantly changed after 48 h at both stress conditions. Furthermore, the mitochondrial ROS levels were elevated in a time-dependent manner up to 6 h and 12 h in the cold and heat stress groups, respectively. The activities of superoxide dismutase, glutathione peroxidase and catalase were successively increased with increasing periods of cold or heat exposure, and their gene expression levels showed similar changes in both stress conditions. Most tested heat shock protein (HSP) genes were sensitive to temperature exposure, and the expression profiles of most apoptosis-related genes was significantly upregulated at 3 and 48 h under cold and heat stress, respectively. Apoptotic index at 48 h under cold stress was significantly higher than that under heat stress. Notably, lipid droplets, , and might be suitable bioindicators of heat stress. The results of these alterations at physiological, biochemical and molecular levels might contribute to a better understanding of the stress response of, and perhaps amphibians more generally, under thermal stress.

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“…When insects suffer from environmental stresses such as extreme temperatures, reactive oxygen species (ROS) are spawned (Gao XM et al, 2013). Metalloenzyme SOD is the most effective intracellular enzymatic antioxidant which is ubiquitous in all aerobic organisms and in all subcellular compartments prone to ROS mediated oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Characterization of a Mn-SOD from the desert beetle Microdera punctipennis and its increased resistance to cold stress in E. coli cells (v0.2)"

Sharma¹

2020

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Insects have developed a complex network of enzymatic antioxidant systems for handling reactive oxygen species (ROS) generated during stress. Superoxide dismutases (SODs) play a determinant role in balancing ROS in insect. However, studies devoted to SODs functions in insects under cold stress are limited. In the present study, we attempted to identify and characterize a mitochondrial manganese SOD (mMn-SOD) from the desert beetle Micordera punctipennis (denoted as MpmMn-SOD) and explore its protective effects on bacteria cells under cold stress. MpmMn-SOD is composed of 202 amino acids with conserved domains required for metal ions binding and enzyme activity. RT-qPCR experiments revealed that the expression of MpmMn-SOD was ubiquitous but tissuespecific and was induced by cold stress. An E. coli (BL21) system was applied to study the function of MpmMn-SOD. The MpmMn-SOD gene was cloned into the prokaryotic expression vector pET-32a to generate a recombinant plasmid pET-32a(MpmMn-SOD). After transformation of the plasmid into E. coli BL21, the fusion protein Trx-His-MpmMn-SOD was overexpressed and identified by SDS-PAGE and Western blotting. Antioxidant activity assay showed that the death zones of the transformed bacteria BL21 (pET32a-mMn-SOD) were smaller in diameter than the control bacteria BL21 (pET32a). Survival curves under-4 ℃ showed that BL21 (pET32a-mMn-SOD) had significant enhanced cold resistance compared to BL21 (pET32a). Its SOD activity under-4 ℃ had a significant negative correlation (r =-0.995) with superoxide anion O 2 •-content. Accordingly, under cold stress BL21 (pET32a-mMn-SOD) had lower electric conductivity and malondialdehyde (MDA) content than BL21 (pET32a). Taken together, our results showed that cold stress stimulated the expression of MpmMn-SOD in M. punctipennis. The E. coli cells that overexpress MpmMn-SOD increase their resistance to cold stress by scavenging ROS, and mitigate potential cell damage caused by ROS under cold conditions.

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Involvement of superoxide dismutase in oxidative stress in the oriental fruit fly,Bactrocera dorsalis: molecular cloning and expression profiles

Abstract: These different expression profiles suggest that the four BdSODs play different roles and respond to different oxidative stresses in B. dorsalis. Some BdSODs undergo specific reaction in the response to specific oxidative stresses.

Cited by 33 publications

References 67 publications

Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee

Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Peer Review #1 of "Characterization of a Mn-SOD from the desert beetle Microdera punctipennis and its increased resistance to cold stress in E. coli cells (v0.2)"

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