Involvement of substance P in scratching behaviour in an atopic dermatitis model

Ohmura, Tsuyoshi; Hayashi, Toshihiro; Satoh, Yoshinobu; Konomi, Ayako; Jung, Birgit; Satoh, Hisashi

doi:10.1016/j.ejphar.2004.03.047

Cited by 82 publications

(61 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that substance P plays crucial roles in both scratching behavior and skin inflammation in NC/Nga mice [16, 17]. We therefore analyzed cutaneous substance P levels in 13-week-old PKGM-fed mice.…”

Section: Resultsmentioning

confidence: 99%

“…Also in NC/Nga mice, frequency of scratching behavior correlates well with severity of dermatitis [13, 14], and prevention of scratching is indeed effective in AD suppression [15]. It is also known that substance P plays an important role in both scratching behavior and skin inflammation in NC/Nga mice [16, 17]. Another hallmark of AD-like phenotype is cutaneous Th2 inflammation, which is often accompanied by simultaneous induction of the Th1 response at the chronic phase [6,7,8,9, 18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Pulverized Konjac Glucomannan Suppresses Scratching Behavior and Skin Inflammatory Immune Responses in NC/Nga Mice

Onishi

Kawamoto²,

Suzuki

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Feeding with pulverized konjac glucomannan (PKGM) suppresses the development of eczema and hyper-IgE production in NC/Nga mice, a model of atopic dermatitis. This study aimed to examine the effects of PKGM on scratching behavior and skin inflammatory immune responses in NC/Nga mice. Methods: Four-week-old NC/Nga mice were maintained for 8 or 9 weeks on diet containing PKGM. Scratching behavior and clinical symptoms were evaluated every 2 weeks. Effects of PKGM on cutaneous inflammation were evaluated by histopathological analysis. Local expression levels of substance P and proinflammatory cytokines were measured by ELISA. Results: An increase in scratching behavior was evident from 6 weeks of age in control mice, but this symptom was dose-dependently inhibited in PKGM-fed mice. Continuous PKGM feeding then significantly inhibited eczematous skin lesions including hyperkeratosis, dermal mastocytosis and eosinophilia. Concomitantly, cutaneous overproductions of substance P, IL-10, IL-4, and TNF-α were all suppressed in PKGM-fed mice. Conclusions: PKGM feeding markedly suppressed development of scratching behavior, substance P expression with mastocytosis, and skin inflammatory immune responses in NC/Nga mice.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Pulverized Konjac Glucomannan Suppresses Scratching Behavior and Skin Inflammatory Immune Responses in NC/Nga Mice

Onishi

Kawamoto²,

Suzuki

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…SP can induce itching, The clinical severity score of the dermatitis was evaluated by the previously described criteria for human AD (Leung et al, 1990 and spantide (a substance P antagonist) inhibits scratching behavior (Andoh et al, 2001;Ohmura et al, 2004). To examine whether or not spantide inhibits stress-induced AD-like skin lesions in NC/Nga mice, we injected 50 nM of spantide intraperitoneally 30 min prior to WAS.…”

Section: The Effect Of Anti-substance P (Sp) On the Onset Of Admentioning

confidence: 99%

Psychological Stress can Trigger Atopic Dermatitis in NC/Nga Mice: An Inhibitory Effect of Corticotropin-Releasing Factor

Amano

Negishi

Akiyama

et al. 2007

Neuropsychopharmacol

View full text Add to dashboard Cite

Atopic dermatitis (AD) is one of the most common inflammatory diseases of the skin and is usually associated with a family history of atopic diathesis. It has been well established that many environmental or psychological factors aggravate AD. However, it is not clear whether psychological stress by itself can trigger AD. We examined the effect of psychological stress on the onset of AD, using an animal model, the NC/Nga mouse. The animals were exposed to the water avoidance stress (WAS) test to induce psychological stress. Additionally, we examined how corticotropin-releasing factor (CRF) affected the development of AD induced by psychological stress. Under specific pathogen-free (SPF) conditions, NC/Nga mice did not develop AD-like skin lesions. In contrast, NC/Nga mice exposed to psychological stress developed AD-like skin lesions along with elevated levels of serum immunoglobulin E even when kept under SPF conditions. The AD-like skin lesions induced by WAS were completely blocked by pretreating the animals with CRF. Our data indicate that a psychological factor is capable of eliciting AD-like skin lesions in NC/Nga mice. It is possible that the inhibitory effect of CRF may be mediated by the functional modification of various cells that have CRF receptors.

show abstract

“…This article describes the investigation of tissue sections of rats after intravenous administration of 5.0 mg/kg [ 14 C]figopitant, a tachykinin neurokinin-1 (NK 1 ) antagonist (Ohmura et al, 2004). The approach applied here involved no additional sample preparation step.…”

Section: Introductionmentioning

confidence: 99%

“…To maintain the high dynamic range and mass accuracy across the whole mass range, an optimized strategy can be used by collecting multiple adjacent single ion monitoring (SIM) windows (Southam et al, 2007), if a continuous long and stable analyte signal is obtained. For LESA-MS, the typical volume used for the microextraction of 1 to 2 l enables the long analyte signal (ϳ20 min) needed for the SIM strategy, which cannot be accomplished by the other surface sampling methods.This article describes the investigation of tissue sections of rats after intravenous administration of 5.0 mg/kg [ 14 C]figopitant, a tachykinin neurokinin-1 (NK 1 ) antagonist (Ohmura et al, 2004). The approach applied here involved no additional sample preparation step.…”

mentioning

confidence: 99%

Investigation of Figopitant and Its Metabolites in Rat Tissue by Combining Whole-Body Autoradiography with Liquid Extraction Surface Analysis Mass Spectrometry

Schadt

Kallbach²,

Almeida

et al. 2011

Drug Metab Dispos

View full text Add to dashboard Cite

ABSTRACT:This article describes the combination of whole-body autoradiography with liquid extraction surface analysis (LESA) and mass spectrometry (MS) to study the distribution of the tachykinin neurokinin-1 antagonist figopitant and its metabolites in tissue sections of rats after intravenous administration of 5.0 mg/kg figopitant. An overview of autoradiography results is presented together with mass spectrometry identification and semiquantification of parent drug and its metabolites based on LESA-MS. The quality and accuracy of data generated by LESA-MS were assessed in comparison with classic tissue extraction, sample cleanup, and high-performance liquid chromatography analysis. The parent drug and the N-dealkylated metabolite M474(1) (BIIF 1148) in varying ratios were the predominant compounds in all tissues investigated. In addition, several metabolites formed by oxygenation, dealkylation, and a combination of oxygenation and dealkylation were identified. In summary, the LESA-MS technique was shown to be a powerful tool for identification and semiquantification of figopitant and its metabolites in different tissues and was complementary to quantitative whole-body autoradiography for studying the distribution. IntroductionQuantitative whole-body autoradiography (QWBA) is the imaging method of choice to investigate the distribution of drug-related radioactivity in all organs and tissues of an intact organism, such as an animal carcass. With this technique, information on the concentration of the entire drug-related radioactivity is gained. However, the actual molecular entity-parent compound or metabolites-and their respective proportions of the radioactivity remains unknown. The conventional approach to identify and quantify parent drug and metabolites in tissues is the preparation of organ homogenates and sample analysis by liquid chromatography combined with radiodetection and tandem mass spectrometry (MS/MS). However, this approach is labor-intensive, and some tissues (e.g., salivary glands) and tissue substructures (e.g., renal inner and outer medulla) can be difficult or even impossible to sample and extract. Surface sampling methods such as desorption electrospray ionization, laser desorption ionization, direct analyses in real time, or matrix-assisted laser desorption ionization can speed up analysis time and cut overall costs compared with classic tissue extraction and enable spatial resolution of different anatomical substructures within a given organ (Reyzer et al., 2003).Recently, a fully automated liquid extraction-based surface sampling method for mass spectrometry (MS) analyses of drugs and metabolites in thin tissue sections has been described (Van Berkel and Kertesz, 2009;Kertesz and Van Berkel, 2010). This liquid extraction surface analysis (LESA) method uses a liquid microjunction probe to extract the analytes directly from the surface followed by automated nano-electrospray analysis. Direct surface sampling methods deal with high sample complexity, because no chromatographic separation ...

show abstract

Involvement of substance P in scratching behaviour in an atopic dermatitis model

Cited by 82 publications

References 16 publications

Dietary Pulverized Konjac Glucomannan Suppresses Scratching Behavior and Skin Inflammatory Immune Responses in NC/Nga Mice

Dietary Pulverized Konjac Glucomannan Suppresses Scratching Behavior and Skin Inflammatory Immune Responses in NC/Nga Mice

Psychological Stress can Trigger Atopic Dermatitis in NC/Nga Mice: An Inhibitory Effect of Corticotropin-Releasing Factor

Investigation of Figopitant and Its Metabolites in Rat Tissue by Combining Whole-Body Autoradiography with Liquid Extraction Surface Analysis Mass Spectrometry

Contact Info

Product

Resources

About