Involvement of specific macrophage-lineage cells surrounding arterioles in barrier and scavenger function in brain cortex.

Mato, Masao; Ookawara, Shigeo; Sakamoto, Atsushi; Aikawa, Eizo; Ogawa, Tsuyoshi; Mitsuhashi, Umehachi; Masuzawa, Toshio; Suzuki, Hiroshi; Honda, Makoto; Yazaki, Yoshio; Watanabe, Eiju; Luoma, Jukka; Ylä‐Herttuala, Seppo; Fraser, Iain P.; Gordon, Siamon; Kodama, Tatsuhiko

doi:10.1073/pnas.93.8.3269

Cited by 167 publications

(152 citation statements)

References 30 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, Matsumoto et al (1990) reported the presence of SR-A in the human brain in 1990. Since then, SR-A has been detected in the microglia (Grewal et al, 1997;El Koury et al, 1998), in senile plaques of brain tissue from patients with Alzheimer's disease (Christie et al, 1996), and on Mato's fluorescent granular perithelial perivascular macrophages in the normal brain tissue (Mato et al, 1996). We observed in this study that SR-A deficiency significantly reduced infarction after cerebral I/R, suggesting that SR-A, that is expressed on the microglia and Mato cells, could mediate pathophysiological response to cerebral I/R injury.…”

Section: Discussionmentioning

confidence: 99%

Scavenger Receptor Class-A Has a Central Role in Cerebral Ischemia–Reperfusion Injury

Chen

Fang

Liu

et al. 2010

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

The innate immune response is involved in the pathophysiology of cerebral ischemia-reperfusion (I/R) injury. Recent evidence suggests that scavenger receptors have a role in the induction of innate immunity. In this study, we examined the role of scavenger receptor A (SR-A) in focal cerebral I/R injury. Both SR-A À/À mice (n = 10) and age-matched wild-type (WT) mice (n = 9) were subjected to focal cerebral ischemia (60 minutes), followed by reperfusion (for 24 hours). Infarct size was determined by TTC (triphenyltetrazolium chloride) staining. The morphology of neurons in the brain sections was examined by Nissl's staining. Activation of intracellular signaling was analyzed by western blot. Cerebral infarct size in SR-A À/À mice was significantly reduced by 63.9% compared with WT mice after cerebral I/R. In SR-A À/À mice, there was less neuronal damage in the hippocampus compared with WT mice. Levels of FasL, Fas, FADD, caspase-3 activity, and terminal deoynucleotidyl transferase-mediated 2 0 -deoxyuridine 5 0 -triphosphate-biotin nick end labelingpositive apoptotic cells were significantly increased in WT mice after cerebral I/R, but not in SR-A À/À mice. Cerebral I/R increased nuclear factor-jB activation in WT mice, but not in SR-A À/À mice. These data suggest that SR-A has a central role in cerebral I/R injury and that suppression of SR-A may be a useful approach for ameliorating brain injury in stroke patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Scavenger Receptor Class-A Has a Central Role in Cerebral Ischemia–Reperfusion Injury

Chen

Fang

Liu

et al. 2010

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…10,21,[63][64][65] Parenchymal microglia are derived from fetal precursors early in development and originate in the yolk sac, although it is not clear whether bone marrow elements or circulating monocytes are differentiated into parenchymal microglia (Glut-1, blue). A large number of CD163 ϩ perivascular macrophages were positive for CD14 and CD16.…”

Section: Cd163 a Monocyte Lineagementioning

confidence: 99%

CD163 Identifies Perivascular Macrophages in Normal and Viral Encephalitic Brains and Potential Precursors to Perivascular Macrophages in Blood

Kim

Álvarez

Fisher

et al. 2006

The American Journal of Pathology

187

204

View full text Add to dashboard Cite

“…The negative controls included incubations with glass-and species-matched unrelated immunoglobulins (Igs) and incubations in which primary Abs were omitted. 23,24 Tumor sizes were determined with a computerized image analysis system (MCID Software, Imaging Research, St. Catharines, Ontario, Canada) from histological sections by measuring the tumor area (pixels) at the level of the injection site. The tumor area was standardized against the total brain area measured from the same section.…”

Section: Immunocytochemistry and Image Analysismentioning

confidence: 99%

Herpes simplex virus thymidine kinase gene therapy in experimental rat BT4C glioma model: Effect of the percentage of thymidine kinase-positive glioma cells on treatment effect, survival time, and tissue reactions

et al. 2000

View full text Add to dashboard Cite

Herpes simplex virus thymidine kinase (HSV-tk) gene transfer and ganciclovir (GCV) administration have been suggested for the treatment of malignant gliomas. To understand tissue responses and possible ways to improve the treatment effect, we studied tumor growth, tissue reactions, and survival time after HSV-tk/GCV treatment in a syngeneic BT4C rat glioma model by mixing various ratios of stably transfected HSV-tk-expressing BT4C-tk glioma cells with wild-type BT4C glioma cells (percentage of BT4C-tk cells: 0%, 1%, 10%, 30%, 50%, and 100%), followed by injection into BDIX rat brains (n ϭ 79). With the exception of some animals with end-stage tumors, very little astroglia or microglia reactivity was detected in the wild-type tumors as analyzed by immunocytochemistry using glial fibrillary acid protein (GFAP)-, vimentin-, human histocompatibility leukocyte antigen-DR-, OX-42-, and CD68-specific monoclonal antibodies. After 14 days of GCV treatment, tumors induced with Ն10% BT4C-tk cells showed a significant reduction in tumor size (P Ͻ .05) and prolonged survival time (P Ͻ .01). Astrogliosis, as indicated by a strong GFAP and vimentin immunoreactivity, was seen in the tumor scar area. GFAP and vimentin reactivity was already present after the GCV treatment in tumors induced with 1% BT4C-tk cells. Much less human histocompatibility leukocyte antigen-DR-positive microglia was seen in the treated animals, indicating low microglia reactivity and immunoactivation against the tumor. However, GCV-treated tumors were positive for apoptosis, indicating that apoptosis is an important mechanism for cell death in the BT4C-tk glioma model. Our results suggest that Ն10% transfection efficiency is required for a successful reduction in BT4C glioma tumor size with HSV-tk/GCV treatment in vivo. Tissue reactions after 14 days of GCV treatment are characterized by astrogliosis and apoptosis, whereas microglia response and immunoactivation of the brain cells appear to play a minor role. Stimulation of the microglia response by gene transfer or other means might improve the efficacy of the HSV-tk/GCV treatment in vivo. Cancer Gene Therapy (2000) 7, 413-421

show abstract

Involvement of specific macrophage-lineage cells surrounding arterioles in barrier and scavenger function in brain cortex.

Abstract: ABSTRACT

Cited by 167 publications

References 30 publications

Scavenger Receptor Class-A Has a Central Role in Cerebral Ischemia–Reperfusion Injury

Scavenger Receptor Class-A Has a Central Role in Cerebral Ischemia–Reperfusion Injury

CD163 Identifies Perivascular Macrophages in Normal and Viral Encephalitic Brains and Potential Precursors to Perivascular Macrophages in Blood

Herpes simplex virus thymidine kinase gene therapy in experimental rat BT4C glioma model: Effect of the percentage of thymidine kinase-positive glioma cells on treatment effect, survival time, and tissue reactions

Contact Info

Product

Resources

About