Involvement of satellite <scp>I</scp> noncoding <scp>RNA</scp> in regulation of chromosome segregation

Ideue, Takashi; Cho, Yukiko; Nishimura, Koshi; Tani, Tohru

doi:10.1111/gtc.12149

Cited by 72 publications

(114 citation statements)

References 31 publications

(62 reference statements)

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“…My results suggest that a high local concentration of centromeric lncRNAs on mitotic centromeres and chromosomes is an additional pathway for CPC localization and activation that acts in parallel to known pathways of CPC recruitment. Additionally, my results suggest that the CPC is one of the major mitotic targets for regulation by cen-RNAs and confirms the observation that noncoding RNAs play an active role during mitosis (Blower et al, 2005; Du et al, 2010; Ferri et al, 2009; Ideue et al, 2014; Jambhekar et al, 2014; Quenet and Dalal, 2014; Rosic et al, 2014). …”

Section: Discussionsupporting

confidence: 83%

“…Recent work in Drosophila demonstrated that the act of transcription, rather than the resulting RNA, is required for normal Cenp-A deposition (Chen et al, 2015). While several other studies have depleted centromeric RNAs and concluded that cen-RNA is required for various mitotic processes (Ideue et al, 2014; Liu et al, 2015; Quenet and Dalal, 2014; Rosic et al, 2014). Taken together, these studies support the hypothesis that transcription of centromeric DNA plays a role in centromere/kinetochore function through both the process of transcription and through the production of a functional cen-RNA.…”

Section: Discussionmentioning

confidence: 98%

“…Recent work in Drosophila, and human and mouse tissue culture cells have indicated that centromeric repeats are transcribed into long noncoding RNAs (lncRNA) and that actively elongating RNA polymerase II is localized to the centromere/kinetochore (Chan et al, 2012; Ideue et al, 2014; Liu et al, 2015; Quenet and Dalal, 2014; Rosic et al, 2014; Wong et al, 2007). Centromeric lncRNAs (cen-RNA) associate with Cenp-A, -C, HJURP, SgoI, and Aurora-B and are required for normal kinetochore assembly.…”

Section: Introductionmentioning

confidence: 99%

“…One of the most important functions of the CPC requires localization to the inner centromere region where it serves as a sensor of normal bipolar attachment to the spindle (Lampson and Cheeseman, 2011). Work in several organisms has demonstrated that the CPC associates with spindle-enriched RNAs (Jambhekar et al, 2014) and with cen-RNAs (Ferri et al, 2009; Ideue et al, 2014). In addition, the CPC binds directly to RNA in vitro and RNA binding is required for normal CPC localization to the inner centromere (Jambhekar et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Centromeric Transcription Regulates Aurora-B Localization and Activation

Blower

2016

Cell Reports

105

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Summary Centromeric transcription is widely conserved, however it is not clear what role centromere transcription plays during mitosis. Here I find that centromeres are transcribed in Xenopus egg extracts into a long noncoding RNA (lncRNA; cen-RNA) that localizes to mitotic centromeres, chromatin, and spindles. Cen-RNAs bind to the Chromosomal Passenger Complex (CPC) in vitro and in vivo. Blocking transcription or antisense inhibition of cen-RNA leads to a reduction of CPC localization to the inner centromere and misregulation of CPC component Aurora-B activation, independently of known centromere recruitment pathways. Additionally, transcription is required for normal bipolar attachment of kinetochores to the mitotic spindle, consistent with a role for cen-RNA in CPC regulation. This work demonstrates that cen-RNAs promote normal kinetochore function through regulation of the localization and activation of the CPC and confirm that lncRNAs are components of the centromere.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Centromeric Transcription Regulates Aurora-B Localization and Activation

Blower

2016

Cell Reports

105

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“…Centromeric transcription during mitosis is necessary for kinetochore assembly and function36 and centromeric transcripts are essential for maintaining a functional kinetochore263844454647. Here, we have shown that transcriptional activation coupled with histone H4 acetylation is not sufficient to maintain a functional kinetochore in the absence of H3K4me2.…”

Section: Resultsmentioning

confidence: 85%

Epigenetic engineering reveals a balance between histone modifications and transcription in kinetochore maintenance

et al. 2016

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Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology ‘in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modules with competing activities are simultaneously targeted to a synthetic alphoidtetO HAC centromere. This allows us to uncouple transcription from histone modifications at the centromere. Here, we report that H3K4me2 loss decreases centromeric transcription, CENP-A assembly and stability and causes spreading of H3K9me3 across the HAC, ultimately inactivating the centromere. Surprisingly, CENP-28/Eaf6-induced transcription of the alphoidtetO array associated with H4K12 acetylation does not rescue the phenotype, whereas p65-induced transcription associated with H3K9 acetylation does rescue. Thus mitotic transcription plus histone modifications including H3K9ac constitute the ‘epigenetic landscape' allowing CENP-A assembly and centrochromatin maintenance. H3K4me2 is required for the transcription and H3K9ac may form a barrier to prevent heterochromatin spreading and kinetochore inactivation at human centromeres.

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An emerging role of transcription in chromosome segregation: Ongoing centromeric transcription maintains centromeric cohesion

Chen

Liu

2021

BioEssays

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Non-coding centromeres, which dictate kinetochore formation for proper chromosome segregation, are extremely divergent in DNA sequences across species but are under active transcription carried out by RNA polymerase (RNAP) II. The RNAP IImediated centromeric transcription has been shown to facilitate the deposition of the centromere protein A (CENP-A) to centromeres, establishing a conserved and critical role of centromeric transcription in centromere maintenance. Our recent work revealed another role of centromeric transcription in chromosome segregation: maintaining centromeric cohesion during mitosis. Interestingly, this role appears to be fulfilled through ongoing centromeric transcription rather than centromeric transcripts.In addition, we found that centromeric transcription may not require some of the traditional transcription initiation factors, suggestive of "uniqueness" in its regulation. In this review, we discuss the novel role and regulation of centromeric transcription as well as the potential underlying mechanisms.

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Involvement of satellite I noncoding RNA in regulation of chromosome segregation

Cited by 72 publications

References 31 publications

Centromeric Transcription Regulates Aurora-B Localization and Activation

Centromeric Transcription Regulates Aurora-B Localization and Activation

Epigenetic engineering reveals a balance between histone modifications and transcription in kinetochore maintenance

An emerging role of transcription in chromosome segregation: Ongoing centromeric transcription maintains centromeric cohesion

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