Involvement of putative glutamate receptors in plant defence signaling and NO production

Vatsa, Parul; Chiltz, Annick; Bourque, Stéphane; Wendehenne, David; Garcia-Brugger, Angela; Pugin, Alain

doi:10.1016/j.biochi.2011.04.006

Cited by 67 publications

(73 citation statements)

References 32 publications

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“…The NO-induced Pro accumulation was a result of enhanced Pro synthesis and decreased degradation in maize (Yang and Gong, 2009). Although a relationship between the NO and the Pro precursor Glu was found in tobacco cells, where the involvement of a Glu-receptor in NO production and plant defence signalling was observed (Vatsa et al, 2011), the pattern of saltand NO-dependent changes for Pro and Glu was not similar in maize in the present experiment. This difference can be explained by the possible simultaneous use of Glu for Pro, GSH and/or polyamine synthesis.…”

Section: Gene Expression Studiescontrasting

confidence: 77%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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Abstract:It was assumed that salt-induced redox changes affect amino acid metabolism in maize (Zea mays L.), and this influence may be modified by NO. The applied NaCl treatment reduced the fresh weight of shoots and roots. This decrease was smaller after the combined application of NaCl and an NO-donorETA/NO) in the shoots, while it was greater after simultaneous treatment with NaCl and nitro-l-arginine (l-NNA, inhibitor of NO synthesis) in the roots. The quantum yield efficiency of photosystem II was not influenced by the treatments. NaCl had a significant effect on the redox environment in the leaves as it was shown by the increase in the amount of glutathione disulphide and in the redox potential of the glutathione/glutathione disulphide redox pair. This influence of NaCl was modified by DETA/NO and l-NNA. Pharmacological modification of NO levels affected salt-induced changes in both the total free amino acid content and in the free amino acid composition. NaCl alone increased the concentration of almost all amino acids which effect was strengthened by DETA/NO in the case of Pro. l-NNA treatment resulted in a significant increase in the Ala, Val, Gly and Tyr contents. The Ile, Lys and Val concentrations rose considerably after the combined application of NaCl and DETA/NO compared to NaCl treatment alone in the recovery phase. NaCl also increased the expression of several genes related to the amino acid and antioxidant metabolism, and this effect was modified by DETA/NO. In conclusion, modification of NO levels affected salt-induced, glutathione-dependent redox changes and simultaneously the free amino acid composition and the level of several free amino acids. The observed much higher Pro content in plants treated with both NaCl and DETA/NO during recovery may contribute to the protective effect of NO against salt stress.

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Section: Gene Expression Studiescontrasting

confidence: 77%

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Boldizsár¹,

Simon‐Sarkadi²,

Szirtes³

et al. 2013

Journal of Plant Physiology

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“…Interestingly, it is possible that glutamate itself is a GLR-activating ligand (Chiu et al, 2002;Qi et al, 2006;Forde and Lea, 2007;Stephens et al, 2008). The fungal PAMP cryptogein can elicit an extracellular rise in glutamate and [Ca 2+ ] cyt that is driven by exocytosis (Vatsa et al, 2011), suggesting that glutamate release from the cell is downstream of PAMP perception (Weiland et al, 2016). This might provide a mechanism by which BAK1-mediated glutamate release could stimulate GLR activation.…”

Section: Discussionmentioning

confidence: 99%

Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding

et al. 2017

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A transient rise in cytosolic calcium ion concentration is one of the main signals used by plants in perception of their environment. The role of calcium in the detection of abiotic stress is well documented; however, its role during biotic interactions remains unclear. Here, we use a fluorescent calcium biosensor (GCaMP3) in combination with the green peach aphid (Myzus persicae) as a tool to study Arabidopsis thaliana calcium dynamics in vivo and in real time during a live biotic interaction. We demonstrate rapid and highly localized plant calcium elevations around the feeding sites of M. persicae, and by monitoring aphid feeding behavior electrophysiologically, we demonstrate that these elevations correlate with aphid probing of epidermal and mesophyll cells. Furthermore, we dissect the molecular mechanisms involved, showing that interplay between the plant defense coreceptor BRASSINOSTEROID INSENSITIVE-ASSOCIATED KINASE1 (BAK1), the plasma membrane ion channels GLUTAMATE RECEPTOR-LIKE 3.3 and 3.6 (GLR3.3 and GLR3.6), and the vacuolar ion channel TWO-PORE CHANNEL1 (TPC1) mediate these calcium elevations. Consequently, we identify a link between plant perception of biotic threats by BAK1, cellular calcium entry mediated by GLRs, and intracellular calcium release by TPC1 during a biologically relevant interaction.

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“…Although DND1 is important for cytosolic Ca 2+ elevation in response to bacterial lipopolysaccharides and endogenous danger peptides (Ma et al, 2009;Qi et al, 2010), it is not required for flg22 and elf18 activation of Ca 2+ (Jeworutzki et al, 2010). Similarly, the recently suggested Glu receptor-like-type Ca 2+ channels have been implicated in cryptogein-and flg22-triggered responses by pharmacological approaches; however, genetic evidence for their involvement in MAMP signaling is still lacking (Kwaaitaal et al, 2011;Michard et al, 2011;Vatsa et al, 2011). Ca 2+ homeostasis is also controlled through the function of Ca 2+ ATPases, and our data show that FLS2 forms a complex with ACA8.…”

Section: Discussionmentioning

confidence: 99%

“…A number of ion channels have been identified, some of which have roles in plant immunity, such as DEFENSE NO DEATH1 (DND1; Clough et al, 2000;Lamotte et al, 2004;Kudla et al, 2010). Recently, ionotropic Glu receptor-like proteins were shown to regulate Ca 2+ influx at the plasma membrane and were also implicated in MAMP-induced responses (Cho et al, 2009;Kwaaitaal et al, 2011;Michard et al, 2011;Vatsa et al, 2011), and an endoplasmic reticulum-localized P2A-type Ca 2+ ATPase was described to contribute to pathogen-induced cell death and to alter the MAMP-triggered Ca 2+ burst (Zhu et al, 2010). The relevance of the Ca 2+ influx in MAMPelicited responses is underlined by polysaccharides secreted from bacterial pathogens to chelate Ca 2+ in the apoplastic space (Aslam et al, 2008).…”

mentioning

confidence: 99%

Plasma Membrane Calcium ATPases Are Important Components of Receptor-Mediated Signaling in Plant Immune Responses and Development

Mbengue²,

et al. 2012

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Plasma membrane-resident receptor kinases (RKs) initiate signaling pathways important for plant immunity and development. In Arabidopsis (Arabidopsis thaliana), the receptor for the elicitor-active peptide epitope of bacterial flagellin, flg22, is encoded by FLAGELLIN SENSING2 (FLS2), which promotes plant immunity. Despite its relevance, the molecular components regulating FLS2-mediated signaling remain largely unknown. We show that plasma membrane ARABIDOPSIS-AUTOINHIBITED Ca 2+ -ATPase (ACA8) forms a complex with FLS2 in planta. ACA8 and its closest homolog ACA10 are required for limiting the growth of virulent bacteria. One of the earliest flg22 responses is the transient increase of cytosolic Ca 2+ ions, which is crucial for many of the well-described downstream responses (e.g. generation of reactive oxygen species and the transcriptional activation of defense-associated genes). Mutant aca8 aca10 plants show decreased flg22-induced Ca 2+ and reactive oxygen species bursts and exhibit altered transcriptional reprogramming. In particular, mitogen-activated protein kinase-dependent flg22-induced gene expression is elevated, whereas calcium-dependent protein kinase-dependent flg22-induced gene expression is reduced. These results demonstrate that the fine regulation of Ca 2+ fluxes across the plasma membrane is critical for the coordination of the downstream microbe-associated molecular pattern responses and suggest a mechanistic link between the FLS2 receptor complex and signaling kinases via the secondary messenger Ca 2+ . ACA8 also interacts with other RKs such as BRI1 and CLV1 known to regulate plant development, and both aca8 and aca10 mutants show morphological phenotypes, suggesting additional roles for ACA8 and ACA10 in developmental processes. Thus, Ca 2+ ATPases appear to represent general regulatory components of RK-mediated signaling pathways.

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Involvement of putative glutamate receptors in plant defence signaling and NO production

Cited by 67 publications

References 32 publications

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Nitric oxide affects salt-induced changes in free amino acid levels in maize

Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding

Plasma Membrane Calcium ATPases Are Important Components of Receptor-Mediated Signaling in Plant Immune Responses and Development

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