Involvement of pro- and antinociceptive factors in minocycline analgesia in rat neuropathic pain model

Abstract

In neuropathic pain the repeated minocycline treatment inhibited the mRNA and protein expression of the microglial markers and metalloproteinase-9 (MMP-9). The minocycline diminished the pronociceptive (IL-6, IL-18), but not antinociceptive (IL-1alpha, IL-4, IL-10) cytokines at the spinal cord level. In vitro primary cell culture studies have shown that MMP-9, TIMP-1, IL-1beta, IL-1alpha, IL-6, IL-10, and IL-18 are of microglial origin. Minocycline reduces the production of pronociceptive factors, resulting in a more potent antinociceptive effect. This change in the ratio between pro- and antinociceptive factors, in favour of the latter may be the mechanism of minocycline analgesia in neuropathy.

IL-1 receptor antagonist improves morphine and buprenorphine efficacy in a rat neuropathic pain model

“…Microglia and macrophages have been identified as the major sources of IL-1beta (Giulian et al, 1986 andTouzani et al, 1999). Our results and those of others show that microglia are predominantly and profoundly activated in the ipsilateral lumbar spinal cord after peripheral nerve injury (Coyle, 1998, Mika et al, 2007, Piao et al, 2006, Rojewska et al, 2014. It has been hypothesized that activated spinal microglia release pronociceptive cytokines, such as IL-1beta, TNF-alpha, and IL-6 (Hanisch, 2002, Ledeboer et al, 2005, Zanjani et al, 2006and Mika et al, 2008.…”

Treatment with a carbon monoxide-releasing molecule (CORM-2) inhibits neuropathic pain and enhances opioid effectiveness in rats

“…[20] under pentobarbital (60 mg/kg; ip) anesthesia as it 71 has been reported previously [21]. After catheter implantation, 72 the rats were monitored for physical impairments and allowed 73 to recover for a minimum of 1 week before the actual 74 experiment.…”

“…processing, as described previously [21]. Blots were incubated 125 overnight at 4 8C with primary antibodies: anti-IBA-1 (rabbit anti-126 rat, 1:1000, Proteintech), anti-GFAP (rabbit anti-rat, 1:10,000, 127…”

Dataset of botulinum toxin A influence on interleukins under neuropathy

“…Minocycline hydrochloride (30 mg/kg intraperitoneal, Sigma, USA); scheme administration as described before in Rojewska et al [4]Botulinum neurotoxin serotype A (300 pg of toxin in 0.9% NaCl per paw; a gift from Prof. C. Montecucco and from Prof. O. Rossetto, Padova, Italy); administration as described in Mika et al [3]…”