Involvement of position-147 for HLA-E expression

Matsunami, Katsuyoshi; Kusama, Tadashi; Okura, Eiji; Shirakura, R; Fukuzawa, Masahiro; Miyagawa, Shuji

doi:10.1016/j.bbrc.2006.06.146

Cited by 17 publications

(6 citation statements)

References 22 publications

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“…The constructed plasmid was then cloned into the pCXN2L (β-actin promoter + neomycin resistance) expression vector [10]. The plasmid was separately transformed into Escherichia coli DH5α and amplified using standard techniques.…”

Section: Construction Of the Plasmidsmentioning

confidence: 99%

Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G

Esquivel

Maeda

Eguchi

et al. 2015

Transplant Immunology

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Section: Construction Of the Plasmidsmentioning

confidence: 99%

Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G

Esquivel

Maeda

Eguchi

et al. 2015

Transplant Immunology

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“…islets was not different from that in wild-type islets in a dual islet transplantation model, suggesting that antibody-independent mechanisms are important in CXR (17). Thus, it is important to develop strategies to suppress the action of innate immune cells, such as macrophages and neutrophils, for successful clinical application of xenotransplantation (18)(19)(20)(21)(22)(23)(24). Surfactant protein-A (SP-A) and SP-D are epithelial cell-derived immune modulators that are C-type collagen-like lectins.…”

Section: Suppression Of Xenogeneic Innate Immune Response By a Membra...mentioning

confidence: 99%

Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A

et al. 2022

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Macrophage-mediated xenogeneic rejection is a major immunological obstacle. We recently reported that membrane-type surfactant protein-D (SP-D) on swine endothelial cells (SECs) suppressed macrophage-mediated rejection. Similar to SP-D, the carbohydrate recognition domain of surfactant protein-A (SP-A) can induce inhibitory signals in effector cells. The present study aimed to examine the suppressive effect of SP-A on macrophage-mediated xenogeneic rejection. Naive SECs and SPA-transfected SECs (SEC/SP-A) were co-cultured with THP-1 cells and cytotoxicity was evaluated. To investigate the effect of SP-A on phagocytosis, human macrophages were co-cultured with SEC or SEC/SP-A, and the extent of phagocytosis and production of reactive oxygen species were assessed via flow cytometry. The mRNA expression levels of inflammatory cytokines in macrophages were determined using reverse transcription-PCR. Additionally, the effects of THP-1-Lucia NF-κB cells on transcription factors were evaluated. The cytotoxicity and phagocytosis of SEC/SP-A were significantly decreased compared with those of naive SEC. Furthermore, the co-culture of human macrophages with SEC/SP-A decreased reactive oxygen species production, and the mRNA expression levels of TNFα were decreased in macrophages, whereas those of IL-10 were increased. In addition, NF-κB transcription was decreased in SEC/SP-A compared with that in SEC. In conclusion, the ectopic expression of human SP-A in porcine cells represents an attractive method for suppressing macrophage-mediated cytotoxicity.

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“…After overcoming HAR and DXR, the xenograft might become susceptible to cellular rejection, which is mediated by monocytes, macrophages, NK cells and T cells. It is well known that human NK cells induce extensive cell lysis of porcine endothelial cells (PEC) and play an important role in xenogeneic rejection [22][23][24] . Therefore, we studied the suppressive function of human MDSCs against NK-mediated xenogeneic cell lysis [13] .…”

Section: Monocytic Mdscs From Peripheral Blood Suppress Both Nk and Cmentioning

confidence: 99%

Therapeutic potential of monocytic MDSCs from peripheral blood monocytes in transplant rejection

Maeda

Sakai²,

Eguchi³

et al. 2016

Macrophage

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Myeloid-derived suppressor cells (MDSCs) were initially found to contribute to immunosuppression in tumor patients and have recently been recognized as a subset of innate immune cells. They are a heterogeneous population composed of monocytic MDSCs (M-MDSCs) and granulocytic MDSCs (G-MDSCs). In addition, MDSCs have been reported to induce immunological tolerance and prolong the graft survival in transplantation. In mouse CD11b + Gr-1 + cells, MDSCs have been reported to exhibit immunosuppressive functions under various inflammatory conditions. On the other hand, human MDSCs do not have a universal marker and have not been well defined. Furthermore, while mouse CD11b + Gr-1 + MDSCs account for 20-30% of bone marrow cells, human peripheral blood includes only a few percent of MDSCs. Hence, further investigations of human MDSCs and the development of strategies for isolating human MDSCs are mandatory for their use in the clinic for transplantation. We previously established a new strategy to generate monocytic MDSCs in vitro and to isolate them from human peripheral blood mononuclear cells (PBMC). Based on these studies, we have concluded that they have great potential for suppressing cytotoxic T lymphocyte (CTL)-mediated xenocytotoxicity and NK-mediated xenocytotoxicity. Furthermore, they also suppress macrophage-mediated xenocytotoxicity significantly. Here, we review the therapeutic potential of MDSCs in transplantation.

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Involvement of position-147 for HLA-E expression

Cited by 17 publications

References 22 publications

Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G

Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G

Suppression of xenogeneic innate immune response by a membrane‑type human surfactant protein‑A

Therapeutic potential of monocytic MDSCs from peripheral blood monocytes in transplant rejection

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