Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels

Mellouk, Namya; Ramé, Christelle; Touzé, Jean-Luc; Briant, Eric; Ma, Lu; Guillaume, Daniel; Lomet, Didier; Caraty, Alain; Ntallaris, Theodoros; Humblot, P.; Dupont, Joëlle

doi:10.3168/jds.2017-12657

Cited by 21 publications

(39 citation statements)

References 64 publications

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“…During the bovine oestrus cycle, serum adiponectin levels range between 27 and 32 μg/ml and were 1. follicular fluid, but the concentrations in the serum and follicular fluid were not correlated [33]. The circulating levels of adiponectin were decreased when providing low energy diet to the cows, and were related to a delayed and abnormal luteal activity in lactating cows [34]. In vitro, adiponectin (3 μg/ml) decreases the production of androstenedione (A4) by TC in cows by reducing the expression of LH receptors and CYP11A1 (cytochrome P450, family 11, subfamily a, polypeptide 1) and CYP17α1 (cytochrome P450, family 17, subfamily a, polypeptide 1) enzymes mediated by both receptors AdipoR1 and AdipoR2.…”

Section: Adiponectinmentioning

confidence: 88%

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

Kurowska¹,

Mlyczyńska²,

Barbe³

et al. 2018

New Insights Into Theriogenology

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Currently, it is clear that female reproduction is regulated by the hypothalamic-pituitaryovary axis, which produces many hormones that control reproductive stages. It is therefore important to have knowledge of new regulators/hormones controlling reproduction in domestic animals. In female animals, energy metabolism and fertility are tightly connected, and reciprocally regulated. The adipose tissue is well known to be implicated in the secretion of several hormones, such as the adiponectin, resistin, chemerin, visfatin, vaspin and apelin, the so-called adipokines or "adipose tissue-derived hormones". Many reports indicate that adipokines regulate the ovarian follicles' development, the onset of puberty and/or ovulation. This chapter summarizes that several adipokines are expressed in the ovary and that they can regulate ovarian physiology such as the steroid hormone production, cell proliferation, apoptosis and oocyte maturation in different domestic animals like pigs, cows, goats, ewes, chickens and turkeys.

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Section: Adiponectinmentioning

confidence: 88%

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

Kurowska¹,

Mlyczyńska²,

Barbe³

et al. 2018

New Insights Into Theriogenology

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“…These findings are in agreement with Dubuc et al (2011) and reinforce the subclinical nature of this condition. In dairy cows, plasma concentrations of adipokines are affected by negative energy balance status ( Mellouk et al 2017 ), variations in white adipose tissue quantities ( Reverchon et al 2014 ) and milk yield ( Kafi et al 2015 ). However, no differences were observed here between uterine health groups regarding these parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Plasma and uterine fluid total ADIPOQ, RARRES2 and NAMPT concentrations were measured by using ELISA kits (E11A0125 and E11C0104 from BlueGene, Shanghai, China and EK-003-80 from Phoenix France, SAS, Strasbourg, France, respectively), as previously described ( Fadden & Bobe 2016 , Mellouk et al 2017 , 2019 ). The intra-assay coefficients of variation for total ADIPOQ, RARRES2 and NAMPT were 6%, 4.5% and 6.5%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows

et al. 2020

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Adipokines emerged as regulators of metabolism and inflammation in several scenarios. This study evaluated the relationship between adipokines (adiponectin, chemerin and visfatin) and cytological (subclinical) endometritis, by comparing healthy (without), transient (recovered by 45 days postpartum (DPP)) and persistent (until 45 DPP) endometritis cows (n = 49). Cows with persistent endometritis had higher adiponectin concentrations in plasma (at 21 DPP, P < 0.05 and at 45 DPP, P < 0.01) and in uterine fluid (at 45 DPP, P < 0.001), and higher chemerin concentrations in plasma (P < 0.05) and uterine fluid (P < 0.01) at 45 DPP, than healthy cows. Cows with persistent endometritis had higher gene transcription in the cellular pellet of uterine fluid and protein expression in the endometrium of these adipokines and their receptors than in healthy cows. Adiponectin plasma concentrations allowed to discriminate healthy from persistent endometritis cows, in 87% (21 DPP) and 98% (45 DPP) of cases, and adiponectin and chemerin uterine fluid concentrations at 45 DPP allowed for this discrimination in 100% of cases. Cows with concentrations above the cutoff were a minimum of 3.5 (plasma 21 DPP), 20.4 (plasma 45 DPP), and 33.3 (uterine fluid 45 DPP) times more at risk of evidencing persistent endometritis at 45 DPP than cows with concentrations below the cutoff. Overall, results indicate a relationship between adipokine signaling and the inflammatory status of the postpartum uterus of dairy cows, evidencing that adipokines represent suitable biomarkers of subclinical endometritis, able to predict the risk of persistence of inflammation.

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“…pp. The composition of the HE and LE diets was previously described [25] (Additional file 1: Table S1). The energy content of the diet was about 1.16-fold higher in the HE than in the LE diet (84.23 vs. 72.35 Mcal/kg of dry matter).…”

Section: Methodsmentioning

confidence: 99%

“…The extent and duration of NEB may lead to some reproductive dysfunctions such as a reduced pulsatile LH release [23], and ovulatory failures [24]. These events are associated with modifications in adipokines (resistin, adiponectin and leptin) blood/serum or plasma concentrations, which are regulated by the energy level of the diet in dairy cows [25].…”

Section: Introductionmentioning

confidence: 99%

Possible involvement of the RARRES2/CMKLR1-system in metabolic and reproductive parameters in Holstein dairy cows

Mellouk

Ramé

Diot

et al. 2019

Reprod Biol Endocrinol

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Background In dairy cows, the energy cost of milk yield results in a negative energy balance (EB) and body fat mobilization that impairs reproductive efficiency. Emerging evidence suggests that the novel adipokines, Retinoic acid receptor responder protein 2 (RARRES2), and its main receptor, Chemokine-like receptor 1 (CMKLR1) are involved in the regulation of metabolic and ovarian functions. So, we investigated in a first experiment the plasma RARRES2, and RARRES2 and CMKLR1 mRNA expression levels in subcutaneous adipose tissue (SAT) and granulosa cells (GC) at different times of body fat mobilization in dairy cows (4, 8, 20 and 44 weeks postpartum, wk. pp. for SAT and 8, 20 and 44 wk. pp. for GC). Then, in a second experiment we examined the effect of high (HE) and low energy (LE) diets on the RARRES2 system and its links with metabolic and reproductive parameters. Methods The first experiment included 9 animals fed with HE diet from 4 to 44 wk. pp. and the second one included animals fed either a HE diet ( n = 8) or a LE diet ( n = 8) from − 4 to 16 wk. peripartum. In both experiments, various metabolic and reproductive parameters were determined and associated with plasma RARRES2 as measured by bovine ELISA. RARRES2 and CMKLR1 mRNA expression levels were analyzed by RT-qPCR in SAT after biopsy and GC after aspiration of follicles. Results Plasma RARRES2 levels were higher at 4 wk. pp. as compared to 20 and 44 wk. pp. and they were positively correlated with body fat mobilization and milk yield. RARRES2 and CMKLR1 mRNA expression levels increased from 4 to 8 wk. pp. (fat mobilization, EB < 0) and remained unchanged at 20 and 44 wk. pp. (fat reconstitution, EB > 0) as compared to 4 wk. pp. in SAT. RARRES2 and CMKLR1 mRNA levels decreased from 8 to 44 wk. pp. in GC from small follicles. In the second experiment, plasma RARRES2 increased from − 4 to 8 wk. peripartum similarly in both LE and HE cows. In addition, the area under of plasma RARRES2 curve was highly negatively associated with the number of small follicles obtained in HE animals during the cycle before the first artificial insemination. In SAT of HE cows, RARRES2 mRNA expression decreased at 1 wk. pp. compared to − 4 and 16 wk. peripartum whereas opposite expression patterns were obtained for CMKLR1. Similar results were observed for CMKLR1 mRNA expression in LE cows while there was no variation in RARRES2 mRNA expression. Moreover, RARRES2 mRNA was higher expressed in LE than in HE cows at 1 wk. pp. Conclusions The lactation-induced fat and energy mobilization influenced plasma RARRES2 profile and mRNA expression pattern of RARRES2 and CMKLR1 similarly in both SAT and GC. In addition, the energy content of the diet did not affect plasma RARRES2 but it altered RARRES2 mRNA expression in SAT and the area under the curve of plasma RARRES2 that was negatively associated to the number of small follicl...

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Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels

Cited by 21 publications

References 64 publications

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

The Adipokines in Domestic Animal Reproduction: Expression and Role in the Regulation of Ovarian Function

Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows

Possible involvement of the RARRES2/CMKLR1-system in metabolic and reproductive parameters in Holstein dairy cows

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