Involvement of PI3K/Akt signaling in PTTH-stimulated ecdysteroidogenesis by prothoracic glands of the silkworm, Bombyx mori

Gu, Shi‐Hong; Young, Shun-Chieh; Lin, Ju-Ling; Lin, Pei‐Ling

doi:10.1016/j.ibmb.2010.12.004

Cited by 60 publications

(56 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, we demonstrated that PTTH moderately stimulated the phosphorylation of Akt, and that PTTH-stimulated Akt phosphorylation was inhibited by LY294002 (Gu et al, 2011a). In the present study, we further showed that PTTH-stimulated phosphorylation of 4E-BP and S6K is downstream signaling of PI3K.…”

Section: Discussionsupporting

confidence: 76%

“…These results indicate that PTTH-stimulated TOR signaling is ERKindependent. The ERK-independency of PI3K/Akt signaling in PTTH-stimulated prothoracic glands was recently reported (Gu et al, 2011a). Thus PI3K/Akt/TOR and ERK are two distinct signaling pathways involved in PTTH-stimulated ecdysteroidogenesis in B. mori prothoracic glands.…”

Section: Discussionmentioning

confidence: 81%

“…Using a quantitative phosphoproteomics approach to characterize PTTH's action, it was recently demonstrated that in addition to phosphorylation of several known signaling pathways, such as mitogen-activated protein kinase (MAPK), protein kinase A, and protein kinase C, PTTH also regulates several other signal pathways, such as proteins involved in endosomal trafficking, constituents of the cytoskeleton, and the phosphorylation and translation of Halloween protein spook (Rewitz et al, 2009a). Our recent study further showed the involvement of phosphoinositide 3-kinase (PI3K)/Akt signaling in PTTH-stimulated ecdysteroidogenesis by B. mori prothoracic glands (Gu et al, 2011a). However, the downstream signaling of PI3K/Akt is not very clear.…”

Section: Introductionmentioning

confidence: 74%

“…In addition, because we were unable to obtain meaningful blots from Bombyx prothoracic gland lysates with commercially available antitotal 4E-BP and anti-total S6K antibodies, signals obtained with the anti-a-tubulin antibody were used as loading controls. The Western blot analysis followed protocols as described in previous studies (Lin and Gu, 2007;Gu et al, 2009Gu et al, , 2010Gu et al, , 2011aGu et al, , 2011b.…”

Section: Western Blot Analysismentioning

confidence: 99%

See 3 more Smart Citations

TOR signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori

Yeh

Young

et al. 2012

Insect Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 74%

Section: Western Blot Analysismentioning

confidence: 99%

See 2 more Smart Citations

TOR signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori

Yeh

Young

et al. 2012

Insect Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

“…Disruption of the components in PTTH and EcR signaling pathways can cause abnormal development in insects (Gu et al, 2011;Kugler et al, 2011), but it is still unknown how these are correlated with each other at the molecular level. In the present study, we performed a comprehensive screening of genes induced by 20E using DNA microarray …”

Section: Discussionmentioning

confidence: 99%

Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis

et al. 2012

View full text Add to dashboard Cite

Apoptosis of posterior silk gland of Bombyx mori during spinning period and the role of PI3K/Akt pathway

Cheng

et al. 2018

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Bombyx mori is an economic insect of the Lepidoptera. Its posterior silk gland (PSG) is an important organ for fibroin synthesis. In order to study the occurrence of apoptosis in PSG and the role of PI3K/Akt signaling pathway during spinning period, changes in morphology of silk gland, expressions of fibroin components Fib-H, Fib-L and P25 and Akt, TOR2, P70S6K and S6 in PI3K/Akt pathway, expressions of apoptosis related genes caspase-3, caspase-9 and activity of caspase-3 were explored. The results showed that the morphology of silk gland dramatically degenerated; transcription of Fib-H, Fib-L, and P25 gradually declined with time; and Fib-L protein level reduced by 0.6-fold at 72 h. Moreover, the transcription levels of Akt, TOR2, P70S6K, and S6 also decreased by 0.3-, 0.8-, 0.7-, and 0.1-fold, respectively, indicating that the downregulation of PI3K/Akt signaling pathway could lead to reduction in fibroin synthesis. In addition, the transcription levels of caspase-3 and caspase-9 increased by 1.3- and 3.6-fold, respectively, and the enzyme activity of caspase-3 grew at a maximum of 1.6-fold. The results showed the occurrence of apoptosis in PSG during spinning period. In conclusion, the present study indicated that both the decline in fibroin components and the increase in apoptosis-related genes were regulated by PI3K/Akt signaling pathway during spinning period, which shed new light on the functions of PI3K/Akt signaling pathway.

show abstract

Involvement of PI3K/Akt signaling in PTTH-stimulated ecdysteroidogenesis by prothoracic glands of the silkworm, Bombyx mori

Cited by 60 publications

References 43 publications

TOR signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori

TOR signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori

Identification of 20-Hydroxyecdysone-Inducible Genes from Larval Brain of the Silkworm, Bombyx mori, and Their Expression Analysis

Apoptosis of posterior silk gland of Bombyx mori during spinning period and the role of PI3K/Akt pathway

Contact Info

Product

Resources

About