Involvement of Pheophytinase in Ethylene-Mediated Chlorophyll Degradation in the Peel of Harvested ‘Yali’ Pear

Cheng, Yudou; Guan, Junfeng

doi:10.1007/s00344-013-9383-z

Cited by 32 publications

(39 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shi et al reported that exogenous SNP treatment delayed the yellowing of broccoli florets, suppressing CLH activity and CLH1, CLH2, CLH3 gene expression. In Asian and European pears treated with 1‐MCP, downregulation of NYC and NOL expression could play a major role in retarding chlorophyll b degradation . Thus, MeSA had a modulatory effect on degreening.…”

Section: Discussionmentioning

confidence: 99%

“…Chlorophyll degradation is an important physiological catabolism of pigment during plant senescence and fruit ripening. However, in some cultivars, such as ‘Yali’ pear, Thai lime, broccoli, Chinese kale, and spinach, it is a negative characteristic because yellowing resulting from chlorophyll breakdown often leads to a shorter shelf life and diminished commercial value associated with dramatic changes in color, texture, flavor, and aroma of the cultivars, which has a direct effect on food supply and health. It is therefore important to develop techniques that delay or suppress chlorophyll degradation.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro , NOL, rather than NYC1, shows CBR activity . In some plants, like citrus fruit and green lime, CLH‐catalyzed removal of phytol is important for natural chlorophyll degradation, whereas CLH activity is dispensable in other cases, such as in ‘Yali’ pears and Arabidopsis leaves . Recently, PPH was identified as the key enzyme that dephytylates pheophytin to yield pheophorbide, and pph‐1 mutants cause a stay‐green (SGR) phenotype in senescent Arabidopsis leaves .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Methyl salicylate delays peel yellowing of ‘Zaosu’ pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure

et al. 2019

View full text Add to dashboard Cite

BACKGROUND In some cultivars, yellowing resulting from chlorophyll breakdown has a direct and negative effect on food supply and health. The ‘Zaosu’ pear (Pyrus bretschneideri Rehd.), a commercial Asian pear cultivar in China, rapidly turns yellow when stored at room temperature after harvest. To develop techniques that delay or suppress chlorophyll degradation, the effects of methyl salicylate (MeSA) on yellowing in ‘Zaosu’ pear fruit during storage were evaluated. RESULTS Compared with the untreated fruit, the application of 0.05 mmol L−1 MeSA delayed the decline of the total chlorophyll, chlorophyll a and chlorophyll b content, and maintained more intact chloroplasts with fewer and smaller plastoglobuli. Methyl salicylate suppressed enzyme activities, including chlorophyllase, chlorophyll‐degrading peroxidase, Mg dechelatase, and pheophytinase, and the expression levels of NYC, NOL, CLH, SGR, PPH, PAO and RCCR in treated fruit. CONCLUSION Methyl salicylate could delay chlorophyll breakdown in the fruit. The results also suggested that the conversion from chlorophyll a to pheophorbide a could proceed via two pathways, and that alternative pathways for the breakdown of chlorophyll a exist in ‘Zaosu’ pears. © 2019 Society of Chemical Industry

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Methyl salicylate delays peel yellowing of ‘Zaosu’ pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Degreening is a multistep enzymatic process that converts fluorescent Chl into nonfluorescent catabolites (Cheng and Guan 2013). This process is accompanied by degradation of macromolecules and mobilization of nutrients from the senescing leaf to the growing parts of the plant (Martınez and Guiamet 2013;Masclaux-Daubresse and others 2008;Swartzberg and others 2011).…”

Section: Introductionmentioning

confidence: 97%

“…By contrast, salicylic acid, jasmonic acid, abscisic acid, and ethylene accelerate leaf senescence. Shading, temperature, and pathogen attack can also trigger leaf senescence (D'Agostino and Kieber 1999;He and others 2005;Liu and others 2008). However, the timing of leaf senescence is determined by genetic background rather than a passive degenerative process (Buchanan-Wollaston 1997;Swartzberg and others 2011).…”

Section: Introductionmentioning

confidence: 98%

Genetic Dissection for Chlorophyll Content of the Top Three Leaves During Grain Filling in Rice (Oryza sativa L.)

Huang

Dai

Wang

et al. 2015

J Plant Growth Regul

View full text Add to dashboard Cite

Leaf senescence and the consequent decline in chlorophyll (Chl) content is a normal event in the life cycle of plants. Although many studies on Chl content or leaf senescence in rice have been conducted, the understanding of this process is limited. By using a double haploid population derived from a cross between japonica Chunjiang06 (CJ06) and indica Taichong native (TN1), quantitative trait locus (QTL) analysis for Chl content of the top three leaves during the rice grain filling stage was performed in this study. A total of 61 QTLs for the Chl content at four development stages were identified. The QTLs had additive effects of 0.64-3.31 SPAD value with heritability ranging from 2.9 to 26.9 %. Twenty-seven pairs of epistatic interaction for Chl content were estimated and distributed on all 12 chromosomes. The detected QTLs and epistatic interactions were clustered in 15 intervals and expressed with spatial-temporal specificity. The QTLs for leaf senescence and their interactions were further estimated, indicating that the alleles delaying leaf senescence from japonica variety CJ06 exhibited more contribution than those from indica variety TN1. This study revealed that the QTLs for Chl content showed clear spatial-temporal specificity. QTL analysis also showed that more alleles that increased Chl content were contributed from japonica variety CJ06. Thus, the results in this study may help in the selection of the rice variety with delayed leaf Electronic supplementary material The online version of this article (senescence, and will improve understanding of the molecular basis of leaf senescence.

show abstract

Acibenzolar‐S‐methyl activates mitogen‐activated protein kinase cascade to mediate chlorophyll and carotenoid metabolisms in the exocarp of Docteur Jules Guyot pears

Zhu

Cheng

et al. 2022

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND Acibenzolar‐S‐methyl (ASM), a well‐known plant activator, has been used to protect fruit and vegetable from fungal invasion and maintain quality. However, little is known about the molecular mechanism of ASM in regulating chlorophyll and carotenoid metabolisms. Therefore, Docteur Jules Guyot pears were used as the materials to study the changes of hydrogen peroxide (H2O2) production, mitogen‐activated protein kinase (MAPK) cascade, transcription factors, chlorophyll, and carotenoid metabolisms after ASM and PD98059 (a MAPK cascade blocker) treatments. RESULTS ASM increased NADPH oxidase (NOX) and superoxide dismutase (SOD) activities, and H2O2 content, promoted PcMAPKKK1, PcMAPKK3, and PcMAPK6 expressions, and down‐regulated PcMYC2, PcPIF1, PcPIF3, and PcPIF4 expressions in exocarp of pears. ASM also delayed the decrease of chlorophyll a and b contents, and inhibited the accumulation of β‐carotene, lycopene and lutein, PcNYC1, PcHCAR, PcPPH, PcSGR1/2, PcPAO, PcPSY, PcLCYB, PcCRTZ2, PcCCS1 expressions, and promoted PcLCYE expression. PD98059 + ASM treatments depressed SOD and NOX activities and H2O2 content, inhibited PcMAPKKK1, PcMAPKK3, PcMAPK6, PcPIF1, and PcPIF3 expressions, and promoted PcMYC2 and PcPIF4 expressions in exocarp of pears. Additionally, PD98059 + ASM accelerated PcNYC1, PcHCAR, PcPPH, PcSGR1/2, PcPAO, PcPSY, PcCYB, PcCRTZ2, and PcCCS1 expressions, thereby reducing chlorophyll a and b contents, and promoting β‐carotene, lycopene and lutein contents. CONCLUSIONS Postharvest ASM treatment promoted the production of H2O2 to activate the MAPK cascade, then phosphorylated/dephosphorylated transcription factors expression, and delayed chlorophyll decomposition and carotenoid synthesis in pears. © 2022 Society of Chemical Industry.

show abstract

Involvement of Pheophytinase in Ethylene-Mediated Chlorophyll Degradation in the Peel of Harvested ‘Yali’ Pear

Cited by 32 publications

References 55 publications

Methyl salicylate delays peel yellowing of ‘Zaosu’ pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure

Methyl salicylate delays peel yellowing of ‘Zaosu’ pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure

Genetic Dissection for Chlorophyll Content of the Top Three Leaves During Grain Filling in Rice (Oryza sativa L.)

Acibenzolar‐S‐methyl activates mitogen‐activated protein kinase cascade to mediate chlorophyll and carotenoid metabolisms in the exocarp of Docteur Jules Guyot pears

Contact Info

Product

Resources

About