Involvement of Phenoxyl Radical Intermediates in Lipid Antioxidant Action of Myricetin in Iron-Treated Rat Hepatocyte Culture

Stability of baicalin in biological fluids in vitro

“…5) showed that the degradation product was a radical with an apparent EPR signal. The single broad line EPR (g = 2.0045) signal corresponded to phenoxyl radical intermediates as previously reported for other compounds [10]. Because the g value was higher than 2.0023 (the value of carbon-centered radical), we assumed a radical structure where the unpaired electron resides mostly on the oxygen and to a less extent on the carbon atoms.…”

“…Phenoxyl radicals resulted from a one-electron oxidation pathway of compounds. A high number of hydroxyl substituents facilitated unpaired-electron delocalization, leading to a dipolar configuration where multiple mesomeric structures could exist [10]. This was probably the reason why baicalin gave a strong unresolved EPR spectrum corresponding to the envelope of several different spectra.…”

Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plantHedeoma multiflorum

“…Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum liposoluble and water soluble vitamins (E and C), and other compounds such as the amphipatic polyphenols can act as reducing agents, hydrogen donators, singlet oxygen quenchers, and metal chelators (Morel et al,1998;Ulrich-Merzenich et al, 2009).…”

Oxidative Stress Induces Phosphoenolpyruvate Carboxykinase Expression in H4IIE Cells

“…Furthermore, FeNTA decreases the viability of a variety of cells because it strongly induces radicals. 28) Hence we analyzed the direct role of oxidative stress in the expression of PEPCK in rat H4IIE hepatoma cells treated with BSO, which markedly decreases cellular glutathione levels and increases reactive oxygen species without any effect on cell viability. 29,30) H4IIE cells were incubated with 100 or 500 mM of BSO for 24 h and then analyzed by flow cytometry using DCFH-DA.…”