Involvement of Pacific oyster CgPGRP-S1S in bacterial recognition, agglutination and granulocyte degranulation

Iizuka, Masaaki; Nagasaki, Toshihiro; Takahashi, Keisuke; Osada, Makoto; Itoh, Naoki

doi:10.1016/j.dci.2013.10.011

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…In molluscs, rCfPGRPS1 from Chlamys farreri displays affinity to L-PGN from S. aureus ( Yang et al, 2010 ). CgPGRP-S1S from pacific oyster displays specific binding activity to D-PGN, but not to L-PGN ( Iizuka et al, 2014 ). In this study, PGN binding assays revealed that ApPGRPs have PGN-binding activity toward D, L-PGN which was also found in HcPGRPS1 from H. cumingi ( Yang et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Two Novel Short Peptidoglycan Recognition Proteins (PGRPs) From the Deep Sea Vesicomyidae Clam Archivesica packardana: Identification, Recombinant Expression and Bioactivity

Kong

Liu

et al. 2018

Front. Physiol.

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Vesicomyidae clams are common species living in cold seeps, which incorporates symbiotic bacteria into their body maintaining endosymbiosis relationship. As members of pattern recognition receptor (PRR) family, peptidoglycan recognition proteins (PGRPs) recognize pathogen associated molecular patterns and play an important role in innate immunity. In present study, two short PGRPs (ApPGRP-1 and -2) were first identified from Vesicomyidae clam Archivesica packardana. Sequences analysis showed that they have both conserved Zn2+ binding sites (H-H-C) and amidase catalytic sites (H-Y-H-T-C), and phylogenetic tree indicated that they clustered with short PGRPs of other molluscs. PGN assay showed that ApPGRPs could bind Lys-type PGN from Staphylococcus aureus and Dap-type PGN from Bacillus subtilis, and revealed amidase activity with selective zinc ion dependence. rApPGRP-1 and -2 (recombinant ApPGRP-1 and -2) could bind six bacteria with a broad spectrum and had both zinc-dependent and -independent bactericidal activity. ApPGRPs had the complete functions of effectors and partial functions of receptors from PGRPs. Further analyses showed that ApPGRPs from A. packardana might be involved in the endosymbiosis relationship between the host clam and endosymbiotic bacteria as a regulator. The results of these experiments suggested that ApPGRPs were involved in cold seep clams’ immune response. This study provides basic information for further research on the immune mechanisms of deep sea organisms.

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Section: Discussionmentioning

confidence: 99%

Two Novel Short Peptidoglycan Recognition Proteins (PGRPs) From the Deep Sea Vesicomyidae Clam Archivesica packardana: Identification, Recombinant Expression and Bioactivity

Kong

Liu

et al. 2018

Front. Physiol.

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“…PGRPs is considered to be a crucial immune molecule in Molluscs by detecting and eliminating invading bacteria [48][49][50][51][52]. The expression pattern of PGRP has been investigated in Chlamys farreri [49], Solen grandis [51], and Hyriopsis cumingi [52].…”

Section: Discussionmentioning

confidence: 99%

New insights into the Manila clam and PAMPs interaction based on RNA-seq analysis of clam through in vitro challenges with LPS, PGN, and poly(I:C)

Jiang

Nie

et al. 2020

BMC Genomics

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Background: Manila clam (Ruditapes philippinarum) is a worldwide commercially important marine bivalve species. In recent years, however, microbial diseases caused high economic losses and have received increasing attention. To understand the molecular basis of the immune response to pathogen-associated molecular patterns (PAMPs) in R. philippinarum, transcriptome libraries of clam hepatopancreas were constructed at 24 h post-injection with Lipopolysaccharide (LPS), peptidoglycan (PGN), and polyinosinic-polycytidylic acid (poly(I:C)) and phosphatebuffered saline (PBS) control by using RNA sequencing technology (RNA-seq). Results: A total of 832, 839, and 188 differentially expressed genes (DEGs) were found in LPS, PGN, and poly(I:C) challenge group compared with PBS control, respectively. Several immune-related genes and pathways were activated in response to the different PAMPs, suggesting these genes and pathways might specifically participate in the immune response to pathogens. Besides, the analyses provided useful complementary data to compare different PAMPs challenges in vivo. Functional enrichment analysis of DEGs demonstrated that PAMPs responsive signal pathways were related to apoptosis, signal transduction, immune system, and signaling molecules and interaction. Several shared or specific DEGs response to different PAMPs were revealed in R. philippinarum, including pattern recognition receptors (PRRs), antimicrobial peptides (AMPs), interferon-induced proteins (IFI), and some other immune-related genes were found in the present work. Conclusions: This is the first study employing high throughput transcriptomic sequencing to provide valuable genomic resources and investigate Manila clam response to different PAMPs through in vivo challenges with LPS, PGN, and poly(I:C). The results obtained here provide new insights to understanding the immune characteristics of R. philippinarum response to different PAMPs. This information is critical to elucidate the molecular basis of R. philippinarum response to different pathogens invasion, which potentially can be used to develop effective control strategies for different pathogens.

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“…PRRs are an important part of the innate immune response. This general classi cation of immune receptors is de ned by their ability to directly recognize different PAMPs such as lipopolyssacharide (LPS) and peptidoglycan (PGN) from bacteria, β-1,3-glucan from fungi, or double-stranded RNA from viruses [18]. To date, several PPRs (e.g TLRs, brinogen-related proteins, LPS-binding proteins, peptidoglycan recognition proteins and scavenger receptors) have been reported from invertebrates, including C. gigas [19][20][21].…”

Section: Pattern Recognition Receptorsmentioning

confidence: 99%

“…C. gigas TLRs, peptidoglycan recognition proteins (PGRPs), scavenger receptors (SRs), brinogen-like proteins (FREPs), calciumdependent (C-type) mannose receptor, macrophage mannose receptor (MMR), lectins, C-type receptor, protein toll, collectin-12 and hemolymph lipopolysaccharide-binding protein (LPS-BP) were found in higher abundance in challenged groups compared to controls (see Additional le 5: Table S1). Besides TLRs, which are discussed in detail below, other PRRs found in this transcriptome study have also been identi ed in C. gigas [18], Eriocheir sinensis [22], the Sydney Rock Oyster Saccostrea glomerata [23], Limulus polyphemus [24] from PGRPs, SRs, FREPs, C-type mannose receptor, MMR, lectins, and protein toll to collectin-12 and LPS-BP. PGRPs which can recognize peptidoglycan and peptidoglycan-containing bacteria play an indispensable role in innate immunity for invertebrates and vertebrates, due to its outstanding ability in detecting and eliminating invasive bacteria.…”

Section: Pattern Recognition Receptorsmentioning

confidence: 99%

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Fang¹,

Cai²,

Mao³

et al. 2020

Preprint

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Background: Marine molluscs living in intertidal and estuarine areas, such as oysters, face numerous pathogen challenges during their development. Infection from bacteria such as Vibrio alginolyticus, represents a major factor affecting larval development and frequently leads to high mortality of the pacific oyster, Crassostrea gigas. The oyster immune response is known to play an important role in protecting the animal during development by mitigating the consequences of infection. Results: In this study, we undertook a comprehensive analysis of the immune response of C. gigas to V. alginolyticus challenge. We sequenced the transcriptome of C. gigas at 0, 6, 12, 24, 48 and 72 hours post infection. After RNA-seq, the raw reads are available through the NCBI Sequence Read Archive under accession number PRJNA623063. After filtering, a total of 58.24 Gb clean reads were produced and assembled using the reference genome of C. gigas. The distribution of quality Q30 was higher than 90.88% for each sample and the GC content ranged from 41.27% to 42.91%. When compared with sequences in the COG, GO, KEGG, Swiss-Prot, and NR databases, there were 1267, 1112, 2187, 682, 1133 differentially expressed genes annotated at 6, 12, 24, 48, 72 hours post infection respectively. Numerous immune-related genes displayed differential expression that varied over time: toll-like receptors, tripartite motif proteins, Lectin-like factors, scavenger receptors, signaling pathway components such as Myeloid differentiation factor 88, and stress proteins such as Heat shock 70 kDa protein were all found to be higher in abundance following V. alginolyticus challenge compared to control. For analysis, these genes were divided into several categories such as pattern recognition receptors, fibrinogen-like proteins, damage associated molecular patterns, complement factors, etc. These general categories allowed us to generate an immune response profile for C. gigas over the first 72 hours of infection. These results indicate that bacterial infection induce a complex pattern of immune gene expression in C. gigas larvae. Conclusion: Our study will facilitate targeted investigation into the function of specific immune factors that may explain the diversity and evolution of invertebrate immune molecules and lead to the development of effective measures to improve the performance of oyster culture.

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Involvement of Pacific oyster CgPGRP-S1S in bacterial recognition, agglutination and granulocyte degranulation

Cited by 16 publications

References 19 publications

Two Novel Short Peptidoglycan Recognition Proteins (PGRPs) From the Deep Sea Vesicomyidae Clam Archivesica packardana: Identification, Recombinant Expression and Bioactivity

Two Novel Short Peptidoglycan Recognition Proteins (PGRPs) From the Deep Sea Vesicomyidae Clam Archivesica packardana: Identification, Recombinant Expression and Bioactivity

New insights into the Manila clam and PAMPs interaction based on RNA-seq analysis of clam through in vitro challenges with LPS, PGN, and poly(I:C)

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

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