Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells

Yan, Luchun; Huang, Hao; Zhang, Ying; Yuan, Xinrong; Zhou, Yan; Cao, Chunyan; Luo, Xiao-Jun

doi:10.1042/bsr20194065

Cited by 13 publications

(8 citation statements)

References 37 publications

(40 reference statements)

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“…The tumor suppressor gene TP53 is involved in tumor necrosis factor‐α (TNF‐α)‐mediated apoptosis by inducing cell cycle arrest, inhibiting cell proliferation, and ultimately leading to apoptosis. 39 In our experiments, an increase in p53 was also detected at both the transcriptional and translational levels. The above results once again confirmed the inhibitory effect of Nr‐CWS on cervical carcinoma cells.…”

Section: Discussionsupporting

confidence: 69%

“…In vivo and in vitro experiments have demonstrated that Nr‐CWS can reduce the expression levels of E6 and E7 oncoproteins in cervical carcinoma cells and tumor tissues. The tumor suppressor gene TP53 is involved in tumor necrosis factor‐α (TNF‐α)‐mediated apoptosis by inducing cell cycle arrest, inhibiting cell proliferation, and ultimately leading to apoptosis 39 . In our experiments, an increase in p53 was also detected at both the transcriptional and translational levels.…”

Section: Discussionmentioning

confidence: 49%

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Nocardia rubra cell‐wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells

et al. 2022

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Background As an immune enhancer, Nocardia rubra cell‐wall skeleton (Nr‐CWS) has been used to treat persistent human papillomavirus infection and cervical precancerous lesions. However, it is still unclear whether it can be used to treat cervical carcinoma. Methods In our study, the aim was to determine whether Nr‐CWS affects the apoptosis of cervical carcinoma cells by enhancing the antitumor effect of dendritic cells and macrophages in vivo and in vitro. Results The experimental results showed that Nr‐CWS can promote the activity of dendritic cells and macrophages and reduce their apoptosis. It also increased the cytokines IL‐6, IL‐12, TNF‐ɑ, and IL‐1β secreted by dendritic cells and macrophages and reduced their PD‐L1 expression. In vitro, Nr‐CWS inhibited the proliferation, colony forming ability of HeLa and SiHa cervical carcinoma cell lines cultured with macrophages, and more cells were blocked in G2/M phase. Nr‐CWS promoted TNF‐ɑ/TNFR1/caspase‐8‐mediated apoptosis by increasing macrophages secretion of TNF‐ɑ and inhibited cell migration and invasion regulated by the WNT/β‐catenin‐EMT pathway. Nr‐CWS also reduced the expression of the cervical carcinoma genes E6 and E7 thereby increasing expression of p53 gene and decreasing expression of PD‐L1 gene. In vivo, Nr‐CWS inhibited tumor growth and decreased the expression of E6, E7, PD‐L1, P16, Ki67, and PCNA in tumors. Conclusions Therefore, our results suggest that Nr‐CWS can promote apoptosis of cervical carcinoma cells by enhancing the antitumor effect of dendritic cells and macrophages.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 49%

Nocardia rubra cell‐wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells

et al. 2022

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“…Colchicine, a plant-derived alkaloid, could significantly reduce the expression of HPV 16 E6/E7 mRNA and protein in CaSki and HeLa cells. This effect results in up-regulation of tumor suppressor proteins p53 and Rb and down-regulation of phosphorylated Rb (pRb) proteins (Yan et al, 2020).…”

Section: Alkaloidsmentioning

confidence: 99%

Therapeutic Effects of Natural Products on Cervical Cancer: Based on Inflammatory Pathways

Zhou

Long

et al. 2022

Front. Pharmacol.

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Inflammation is a protective response of the body to an irritant. When an inflammatory response occurs, immune cells are recruited to the injury, eliminating the irritation. The excessive inflammatory response can cause harm to the organism. Inflammation has been found to contribute to cervical cancer if there is a problem with the regulation of inflammatory response. Cervical cancer is one of the most common malignant tumors globally, and the incidence tends to be younger. The harm of cervical cancer cannot be ignored. The standard treatments for cervical cancer include surgery, radiotherapy and chemotherapy. However, the prognosis for this treatment is poor, so it is urgent to find a safer and more effective treatment. Natural products are considered excellent candidates for the treatment of cervical cancer. In this review, we first describe the mechanisms by which inflammation induces cervical cancer. Subsequently, we highlight natural products that can treat cervical cancer through inflammatory pathways. We also introduce natural products for the treatment of cervical cancer in clinical trials. Finally, methods to improve the anticancer properties of natural products were added, and the development status of natural products was discussed.

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“…Furthermore, harmine induced apoptosis in breast cancer cells in a time-dependent manner and real-time PCR outcomes on breast cancer cells showed that apoptosis induction in MCF-7 cells by harmine requires the activation of p53 gene expression [ 185 ]. Colchicine exhibits in vitro antiproliferative activity against HPV 16/18-positive human cervical cancer cell lines, which was achieved by HPV E6/E7 inhibition and subsequently p53-dependent intrinsic apoptosis [ 186 ].…”

Section: P53 Signaling Pathway Modulated By Dietary Antioxidantsmentioning

confidence: 99%

Role of Dietary Antioxidants in p53‐Mediated Cancer Chemoprevention and Tumor Suppression

Merlin

Rupasinghe

Dellaire

et al. 2021

Oxidative Medicine and Cellular Longevity

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Cancer arises through a complex interplay between genetic, behavioral, metabolic, and environmental factors that combined trigger cellular changes that over time promote malignancy. In terms of cancer prevention, behavioral interventions such as diet can promote genetic programs that may facilitate tumor suppression; and one of the key tumor suppressors responsible for initiating such programs is p53. The p53 protein is activated by various cellular events such as DNA damage, hypoxia, heat shock, and overexpression of oncogenes. Due to its role in cell fate decisions after DNA damage, regulatory pathways controlled by p53 help to maintain genome stability and thus “guard the genome” against mutations that cause cancer. Dietary intake of flavonoids, a C15 group of polyphenols, is known to inhibit cancer progression and assist DNA repair through p53-mediated mechanisms in human cells via their antioxidant activities. For example, quercetin arrests human cervical cancer cell growth by blocking the G2/M phase cell cycle and inducing mitochondrial apoptosis through a p53-dependent mechanism. Other polyphenols such as resveratrol upregulate p53 expression in several cancer cell lines by promoting p53 stability, which in colon cancer cells results in the activation of p53-mediated apoptosis. Finally, among vitamins, folic acid seems to play an important role in the chemoprevention of gastric carcinogenesis by enhancing gastric epithelial apoptosis in patients with premalignant lesions by significantly increased expression of p53. In this review, we discuss the role of these and other dietary antioxidants in p53-mediated cell signaling in relation to cancer chemoprevention and tumor suppression in normal and cancer cells.

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Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells

Cited by 13 publications

References 37 publications

Nocardia rubra cell‐wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells

Nocardia rubra cell‐wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells

Therapeutic Effects of Natural Products on Cervical Cancer: Based on Inflammatory Pathways

Role of Dietary Antioxidants in p53‐Mediated Cancer Chemoprevention and Tumor Suppression

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