Involvement of p38 Mitogen-Activated Protein Kinase Activation in Bromocriptine-Induced Apoptosis in Rat Pituitary GH3 Cells1

Kanasaki, Haruhiko; Fukunaga, Kohji; Takahashi, Kentaro; Miyazaki, Kohji; Miyamoto, Eishichi

doi:10.1095/biolreprod62.6.1486

Cited by 55 publications

(38 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another report, p38 MAP kinase is activated in GH3 cells during bromocryptine-induced apoptosis [26]. The apoptosis of GH3 cells was also observed following treatment with a dopaminergic neurotoxin, 1-methyl-4-phenylpyridinium ion [27], and with inhibitors of serine/threonine phosphatases 1 and 2A [28,29].…”

Section: Discussionmentioning

confidence: 90%

Effect of Therapeutic Doses of Ionising Radiation on the Somatomammotroph Pituitary Cell Line, GH3

Mareková

Vokurková

et al. 2003

Endocr J

View full text Add to dashboard Cite

Abstract. Ionising radiation is used for the treatment of pituitary tumours as fractionated radiotherapy, where the total dose reaching the tumour area is in the range of 40-50 Gy, or during stereotactic radiosurgery, where the total dose reaching the tumour area during one session is in the range of 20-90 Gy. In this study, we investigated the effect of ionising radiation of 60 Co (dose rate of 3 Gy/min, similar to that used during gamma knife procedure) on the mode of cell death of the somatomammotroph pituitary cell line, GH3, an immortalized cell line derived from a rat pituitary adenoma. We found that the basic mechanism of cell death induced by irradiation of this GH3 cell line by g-rays was programmed cell death -apoptosis. Doses of 20-50 Gy were shown to inhibit proliferation in these cells. 24 hours after irradiation with a dose of 20 and 50 Gy, cells were shown to accumulate in the G 2 /M phase of cell cycle. This cell cycle arrest lasted for at least ten days. Apoptosis was detected 72 hours towards until the end of the study (10 days). However, a significant number of cells were still alive ten days following irradiation. We conclude that ionising radiation doses of 20 and 50 Gy induce pituitary GH3 cell apoptosis following cell cycle arrest in the G 2 /M phase.

show abstract

Section: Discussionmentioning

confidence: 90%

Effect of Therapeutic Doses of Ionising Radiation on the Somatomammotroph Pituitary Cell Line, GH3

Mareková

Vokurková

et al. 2003

Endocr J

View full text Add to dashboard Cite

show abstract

“…PD98059 (10 M and50 M) and GF109203X (5 M), inhibitors of upstream of ERK1/ERK2-MAPK and PKC, 39 respectively, blocked PPF but not adhesion or size increase. While a low dose of SB203580 (10 M), an inhibitor of p38 MAPK 40 and JNK/SAPK 41 pathways, did not affect cell differentiation, a high dose (50 M) of SB blocked the differentiation. Wortmannin (100 nM and 500 nM), an inhibitor of PI3K/Akt pathway, 39 did not affect cell differentiation.…”

Section: Activation Of Erk1/erk2 Is Essential For Full Differentiatiomentioning

confidence: 96%

Fibronectin- and protein kinase C–mediated activation of ERK/MAPK are essential for proplateletlike formation

Jiang

Jia

Cohen

2002

Blood

View full text Add to dashboard Cite

The megakaryoblastic CHRF-288 cell line was used to investigate signal transduction pathways responsible for proplateletlike formation (PPF). The role of fibronectin (FN) and protein kinase C (PKC) activation in PPF were examined. In the presence of serum and phorbol 12-myristate 13-acetate (PMA), a PKC activator, cells exhibited full megakaryocytic differentiation, manifested by adhesion, shape change, increased cell size, polyploidy, PPF, and expression of CD41(+), CD61(+), and CD62P(+). The same morphologic and phenotypic features were observed in serum-free cultures in the presence of FN/PMA. Only partial differentiation occurred when other integrin ligands were substituted for FN. FN alone induced minimal cell adhesion and spreading, while PMA alone induced only polyploidy without adhesion. Signal transduction changes involved the activation of the extracellular signal-regulated protein kinase 1 (ERK1)/ERK2 as well as c-Jun amino-terminal kinase 1 (JNK1)/stress-activated protein kinase (SAPK). Phosphoinositide-3 kinase and p38 were not stimulated under these conditions. Inhibitors were used to identify the causal relationship between signaling pathways and PPF. PD98059 and GF109203X, inhibitors of ERK1/ERK2 pathway and PKC, respectively, blocked PPF, while adhesion, spreading, and polyploidy were normal. These studies show that activation of ERK1/ERK2 mitogen-activated protein kinase pathway plays a critical role in PPF. The elucidation of the signal transduction pathway on megakaryocyte development and PPF is of crucial importance for understanding this unique biological process.

show abstract

“…Recently, both D 2S and D 2L receptor isoforms have been demonstrated to activate, through the involvement of G bg subunits and protein kinase C, the mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) pathway, generally involved in the regulation of cell growth, differentiation and apoptosis (10). In particular, in pituitary tumor cell lines, dopamine agonists have been found to exert a clear anti-proliferative effect, inducing cell death and apoptosis through the D 2 receptor-mediated activation of MAPK and/or ERK pathways (11,12). The main intracellular pathways connected with the activation of dopamine receptors are summarized in Fig.…”

Section: Functional Characteristics Of Dopamine Receptorsmentioning

confidence: 99%

Novel insights in dopamine receptor physiology

Pivonello¹,

Ferone

Lombardi³

et al. 2007

eur j endocrinol

126

103

View full text Add to dashboard Cite

The dopaminergic system has a pivotal role in the central nervous system but also plays important roles in the periphery, mainly in the endocrine system. Dopamine exerts its functions via five different receptors, named D 1 -D 5 , belonging to the category of G protein coupled membrane receptors. Dopamine receptors are heterogeneously expressed in different cells, tissues and organs, where they stimulate or inhibit different functions, including neurotransmission and hormone synthesis and secretion. In particular, the dopamineric system has a pivotal role in the physiological regulation of the hypothalamus-pituitary-adrenal axis. Recent data have demonstrated the expression and function of dopamine receptors not only in endocrine organs but also in endocrine tumors, mainly those belonging to the hypothalamus-pituitary-adrenal axis, and also in the so-called 'neuroendocrine' tumors. These data confirm the important role of the dopaminergic system in this endocrine axis, as well as in the neuroendocrine system. This review summarizes the main structural and functional characteristics of dopamine receptors, emphasizing the most recent novelties, and focused on the physiological and pathological regulation of the hypothalamus-pituitary-adrenal axis by the dopaminergic system. In addition, the recent findings on the relationship between dopamine receptors and neuroendocrine tumors are summarized.European Journal of Endocrinology 156 S13-S21

show abstract

Involvement of p38 Mitogen-Activated Protein Kinase Activation in Bromocriptine-Induced Apoptosis in Rat Pituitary GH3 Cells1

Cited by 55 publications

References 55 publications

Effect of Therapeutic Doses of Ionising Radiation on the Somatomammotroph Pituitary Cell Line, GH3

Effect of Therapeutic Doses of Ionising Radiation on the Somatomammotroph Pituitary Cell Line, GH3

Fibronectin- and protein kinase C–mediated activation of ERK/MAPK are essential for proplateletlike formation

Novel insights in dopamine receptor physiology

Contact Info

Product

Resources

About