Involvement of p38 and c-Jun N-Terminal Protein Kinase in Cardiotoxin III-Induced Apoptosis of K562 Cells

Chen, Xingyong; Hua-xin, Yang; Qu, Su; Liu, Jing; Lv, Ping; Xu, Jianwen; Xu, Kailai

doi:10.1248/bpb.32.583

Cited by 10 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The major anticancer mechanism activated by snake venom toxins is by mitochondria-dependent cell death pathway. Taiwan cobra cardiotoxin-III induced apoptosis by loss of mitochondrial membrane potential, release of cytochrome c, activation of caspase 9 and caspase 3, and altered expression of Bcl-2 family proteins [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of NN-32 toxin from Indian spectacled cobra venom on human breast cancer cell lines

Attarde

Pandit

2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundBreast cancer is the most common cancer which causes significant morbidity and mortality among women worldwide. Lack of medical facilities for early detection, therapeutic strategies for treatment and side effects due to pharmacological compounds have encompassed the need for new therapies mostly from natural sources. A lot of components have been identified from different snake venoms as therapeutic agents. A group of polypeptides (60–70 amino acid residues) called cytotoxins or cardiotoxins present in an elapid family of snakes have a wide variety of pharmaceutical actions and have the tendency to damage a wide variety of cells including cancerous cells. The aim of the present study was to evaluate the cytotoxic effect of NN-32 protein toxin purified from Indian Spectacled Cobra venom against human breast cancer cell lines (MCF-7 and MDA-MB-231).MethodsThe NN-32 toxin was purified by ion exchange chromatography and further by RP-HPLC. The potential anticancer effects of the NN-32 toxin on MCF-7 and MDA-MB-231 cells were evaluated using MTT, anti-proliferation, neutral red (NR) uptake and Lactate Dehydrogenase (LDH) release assay.ResultsThe ion exchange chromatography showed various peaks among fraction no. 35 showing cytotoxic activity and this fraction showed a single peak with retention time 3.6 mins by HPLC using C18 column. The NN-32 toxin induced cytotoxicity in MCF-7 and MDA-MB-231 cells with the IC50 value of 2.5 and 6.7 μg/ml respectively. The NN-32 showed significant cytotoxicity to both the cell lines along with low cytotoxicity to MCF-10A (normal breast epithelial) cells. The cytotoxic effect was further confirmed by the anti-proliferative, NR uptake and LDH release assays.ConclusionThe purified toxin NN-32 from Naja naja venom showed cytotoxic activity against MCF-7 (ER+) and MDA-MB-231(ER-) cells in both dose dependent and time dependent manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of NN-32 toxin from Indian spectacled cobra venom on human breast cancer cell lines

Attarde

Pandit

2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Another important mediator that accompanied apoptosis progression could be JNK1 activity. JNK is activated under various stress stimuli such as toxic drugs, oxidative stress, heat shock, UV radiation, X-rays, and so on [23][24][25]. Previous reports suggested that the function of JNK as a checkpoint for apoptosis induction is negatively regulated by p21 Waf1/CIP1 , and JNK1 activation is attributed to caspase-dependent dissociation of p21 Waf1/CIP1 [26].…”

Section: Discussionmentioning

confidence: 99%

Caspase-independent apoptosis induction of quorum-sensing autoinducer analogs against chronic myeloid leukemia K562

et al. 2011

View full text Add to dashboard Cite

Quorum sensing is defined as the ability of microorganisms to sense their population density via the release of signaling molecules called autoinducers (AIs). Various types of AI analogs were prepared and their antitumor properties against chronic myeloid leukemia (CML) K562 cells were investigated. Two AI analogs induced progressive apoptosis with JNK activation and p21 induction. In addition, this induction of apoptosis is not related to bcr-abl kinase, which sustains CML proliferation. However, the progression of apoptosis was not inhibited by a caspase family inhibitor. These results suggested that AI analogs could induce caspase-independent apoptosis in CML K562.

show abstract

“…It is not only used for biological materials, but is also used to study the morphology of other materials like the structures of semi crystalline polymers or the domain structure of block polymers. 1,2 At present, agarose and polyacrylamide gel electrophoresis are the most commonly used techniques for the separation and characterization of DNA. There are several different stains that can be used to visualize and photograph DNA aer electrophoresis, including uorescent dyes, visible organic dyes and silver.…”

Section: Introductionmentioning

confidence: 99%

A shortcut organic dye-based staining method for the detection of DNA both in agarose and polyacrylamide gel electrophoresis

Cong

Chen

Zhu

et al. 2013

Analyst

View full text Add to dashboard Cite

In this study, we describe a brief, sensitive and safe organic dye-based staining method for the visualization of DNA both in agarose and polyacrylamide gels by using Victoria Pure Blue BO (VPBBO). Down to 0.8-1.6 ng of λ DNA/HindIII markers in agarose gels and 0.4-0.8 ng of pUC18 DNA/Mspl markers in polyacrylamide gels can be successfully detected within 15 and 10 min by the new developed technique, respectively. Moreover, the mechanism of the VPBBO staining was investigated and further confirmed by electrospray ionization mass spectrometry (ESI-MS) and molecular docking. The results indicated that the interaction between VPBBO and DNA is mainly due to groove binding.

show abstract

Involvement of p38 and c-Jun N-Terminal Protein Kinase in Cardiotoxin III-Induced Apoptosis of K562 Cells

Cited by 10 publications

References 30 publications

Cytotoxic activity of NN-32 toxin from Indian spectacled cobra venom on human breast cancer cell lines

Cytotoxic activity of NN-32 toxin from Indian spectacled cobra venom on human breast cancer cell lines

Caspase-independent apoptosis induction of quorum-sensing autoinducer analogs against chronic myeloid leukemia K562

A shortcut organic dye-based staining method for the detection of DNA both in agarose and polyacrylamide gel electrophoresis

Contact Info

Product

Resources

About